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Protein

Snake venom serine protease Dav-PA

Gene
N/A
Organism
Deinagkistrodon acutus (Hundred-pace snake) (Agkistrodon acutus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Snake venom serine protease that has fibrinogenolytic activities. Also possess esterolysis and amidolytic activities.2 Publications

Catalytic activityi

Selective cleavage of Arg-|-Xaa bond.

Enzyme regulationi

Inhibited by NPGB, leupeptin, aprotinin and PMSF, but not by EDTA, SBTI, heparin and hirudin.

Kineticsi

AaV-SP-II.

  1. KM=0.152 µM for TAME2 Publications
  2. KM=265 µM for D-Phe-L-Pip-Arg-p-NA (S-2238)2 Publications
  3. KM=113 µM for D-Pro-Phe-Arg-p-NA (S-2302)2 Publications
  4. KM=28 µM for N-p-Tosyl-Gly-Pro-Arg-p-NA2 Publications
  5. KM=202 µM for N-p-Tosyl-Gly-Pro-Lys-p-NA2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei65 – 651Charge relay system1 Publication
    Active sitei110 – 1101Charge relay system1 Publication
    Active sitei204 – 2041Charge relay system1 Publication

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Fibrinogenolytic toxin, Hemostasis impairing toxin, Hydrolase, Protease, Serine protease, Toxin

    Protein family/group databases

    MEROPSiS01.023.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Snake venom serine protease Dav-PA
    Short name:
    SVSP
    Alternative name(s):
    AaV-SP-I
    AaV-SP-II (EC:3.4.21.-)
    OrganismiDeinagkistrodon acutus (Hundred-pace snake) (Agkistrodon acutus)
    Taxonomic identifieri36307 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeCrotalinaeDeinagkistrodon

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818By similarityAdd
    BLAST
    Propeptidei19 – 246By similarityPRO_0000028363
    Chaini25 – 258234Snake venom serine protease Dav-PAPRO_0000028364Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi31 ↔ 163PROSITE-ProRule annotation1 Publication
    Glycosylationi44 – 441N-linked (GlcNAc...)1 Publication
    Disulfide bondi50 ↔ 66PROSITE-ProRule annotation1 Publication
    Disulfide bondi98 ↔ 256PROSITE-ProRule annotation1 Publication
    Disulfide bondi142 ↔ 210PROSITE-ProRule annotation1 Publication
    Disulfide bondi174 ↔ 189PROSITE-ProRule annotation1 Publication
    Disulfide bondi200 ↔ 225PROSITE-ProRule annotation1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    Secondary structure

    1
    258
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi39 – 446Combined sources
    Beta strandi47 – 5610Combined sources
    Beta strandi59 – 624Combined sources
    Helixi64 – 663Combined sources
    Beta strandi72 – 765Combined sources
    Beta strandi80 – 823Combined sources
    Beta strandi88 – 903Combined sources
    Beta strandi92 – 976Combined sources
    Beta strandi112 – 1187Combined sources
    Beta strandi141 – 1488Combined sources
    Beta strandi150 – 1545Combined sources
    Beta strandi162 – 1698Combined sources
    Helixi171 – 1777Combined sources
    Beta strandi187 – 1915Combined sources
    Beta strandi207 – 2104Combined sources
    Beta strandi213 – 2208Combined sources
    Beta strandi232 – 2376Combined sources
    Helixi238 – 2403Combined sources
    Helixi241 – 2499Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OP0X-ray2.00A25-258[»]
    1OP2X-ray2.10A25-258[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9I8X1.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 249225Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Snake venom subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOVERGENiHBG013304.

    Family and domain databases

    CDDicd00190. Tryp_SPc. 1 hit.
    InterProiIPR009003. Peptidase_S1_PA.
    IPR001314. Peptidase_S1A.
    IPR001254. Trypsin_dom.
    IPR018114. TRYPSIN_HIS.
    IPR033116. TRYPSIN_SER.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9I8X1-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVLIRVLANL LILQLSYAQK SSELVIGGNE CDINEHRFLV AFFNTTGFFC
    60 70 80 90 100
    GGTLINPEWV VTAAHCDSTD FQMQLGVHSK KVLNEDEQTR NPKEKFICPN
    110 120 130 140 150
    KNNNEVLDKD IMLIKLDKPI SNSKHIAPLS LPSSPPSVGS VCRIMGWGSI
    160 170 180 190 200
    TPVKETFPDV PYCANINLLD HAVCQAGYPE LLAEYRTLCA GIVQGGKDTC
    210 220 230 240 250
    GGDSGGPLIC NGQFQGIVSY GAHPCGQGPK PGIYTNVFDY TDWIQRNIAG

    NTDATCPP
    Length:258
    Mass (Da):28,033
    Last modified:April 18, 2012 - v2
    Checksum:iE8E1369C8919CCCB
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti70 – 701D → N in AaV-SP-II.

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF159058 mRNA. Translation: AAF76378.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF159058 mRNA. Translation: AAF76378.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OP0X-ray2.00A25-258[»]
    1OP2X-ray2.10A25-258[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Protein family/group databases

    MEROPSiS01.023.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Phylogenomic databases

    HOVERGENiHBG013304.

    Miscellaneous databases

    EvolutionaryTraceiQ9I8X1.

    Family and domain databases

    CDDicd00190. Tryp_SPc. 1 hit.
    InterProiIPR009003. Peptidase_S1_PA.
    IPR001314. Peptidase_S1A.
    IPR001254. Trypsin_dom.
    IPR018114. TRYPSIN_HIS.
    IPR033116. TRYPSIN_SER.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiVSPP_DEIAC
    AccessioniPrimary (citable) accession number: Q9I8X1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 4, 2001
    Last sequence update: April 18, 2012
    Last modified: September 7, 2016
    This is version 80 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    AaV-SP-I is sequenced from 25-48 and its carbohydrate contents is 9%, whereas AaV-SP-II is sequenced from 25-64 and its carbohydrate contents is 4%.Curated

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.