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Protein

mRNA cap guanine-N7 methyltransferase

Gene

rnmt

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic subunit of the mRNA-capping methyltransferase RNMT:RAM/FAM103A1 complex that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.By similarity

Catalytic activityi

S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.PROSITE-ProRule annotationBy similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei107S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei131S-adenosyl-L-methionine; via carbonyl oxygenPROSITE-ProRule annotation1
Sitei134mRNA cap bindingPROSITE-ProRule annotation1
Sitei140mRNA cap bindingPROSITE-ProRule annotation1
Binding sitei153S-adenosyl-L-methioninePROSITE-ProRule annotation1
Sitei165mRNA cap bindingPROSITE-ProRule annotation1
Binding sitei187S-adenosyl-L-methionine; via amide nitrogenBy similarity1
Binding sitei210S-adenosyl-L-methionine; via carbonyl oxygenBy similarity1
Binding sitei214mRNA capPROSITE-ProRule annotation1
Binding sitei215S-adenosyl-L-methionineBy similarity1
Binding sitei296mRNA capPROSITE-ProRule annotation1
Binding sitei393mRNA capPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processmRNA capping, mRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi2.1.1.56. 6725.

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA cap guanine-N7 methyltransferase (EC:2.1.1.56By similarity)
Alternative name(s):
RG7MT1
mRNA (guanine-N(7)-)-methyltransferase
mRNA cap methyltransferase
Short name:
xCMT1
Gene namesi
Name:rnmt
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-944374. rnmt.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002483261 – 402mRNA cap guanine-N7 methyltransferaseAdd BLAST402

Proteomic databases

MaxQBiQ9I8S2.

Expressioni

Developmental stagei

Expressed both maternally and zygotically. Expression decreases after gastrulation.1 Publication

Interactioni

Protein-protein interaction databases

IntActiQ9I8S2. 2 interactors.

Structurei

3D structure databases

ProteinModelPortaliQ9I8S2.
SMRiQ9I8S2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini55 – 402mRNA cap 0 methyltransferasePROSITE-ProRule annotationAdd BLAST348

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni103 – 104mRNA cap bindingPROSITE-ProRule annotation2

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG081963.
KOiK00565.

Family and domain databases

InterProiView protein in InterPro
IPR016899. mRNA_G-N7_MeTrfase.
IPR004971. mRNA_G-N7_MeTrfase_dom.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF03291. Pox_MCEL. 1 hit.
PIRSFiPIRSF028762. ABD1. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiView protein in PROSITE
PS51562. RNA_CAP0_MT. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9I8S2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDHVLNPEEK VSQTNSESGG ADGAFQHVKG EHSSPKLSAS EKSLPGNTKS
60 70 80 90 100
PLKRKAAEPD SPPKRPRLEE GHGSLVVTHY NELPETGLEI RSQSRIFHLR
110 120 130 140 150
NFNNWMKSAL IGEFVEKVQQ RTRNITVLDL GCGKGGDLLK WRKGGISKLV
160 170 180 190 200
CTDIADVSVK QCEQRYKDMK RKSRNERIFE AEFLTSDSTK ELLSEKYIDP
210 220 230 240 250
EIKFDICSCQ FVYHYSFETY EQADTMLRNA CERLCPGGFF IGTTPDGFEL
260 270 280 290 300
VKRLEASDTN SFGNDVYTVT FEKKGKYPLF GCKYDFSLEE VVNVPEFLVY
310 320 330 340 350
FPVLVEMAKK YQMKLIYKKT FREFFEEKVK NDEQKMLLKR MKALESYPAA
360 370 380 390 400
PNTKLVSGRT EDYEHAQKMV ENGQIKLPLG TLSKSEWDAT SIYLLFAFEK

QA
Length:402
Mass (Da):46,003
Last modified:October 1, 2000 - v1
Checksum:i5370C6C956FD0E07
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti106M → I in AAH94147 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF218795 mRNA. Translation: AAF43145.1.
BC094147 mRNA. Translation: AAH94147.1.
PIRiJC7199.
RefSeqiNP_001082004.1. NM_001088535.1.
XP_018121821.1. XM_018266332.1.
XP_018121822.1. XM_018266333.1.
XP_018121823.1. XM_018266334.1.
UniGeneiXl.649.

Genome annotation databases

GeneIDi398173.
KEGGixla:398173.

Similar proteinsi

Entry informationi

Entry nameiMCES_XENLA
AccessioniPrimary (citable) accession number: Q9I8S2
Secondary accession number(s): Q52KX9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: October 1, 2000
Last modified: August 30, 2017
This is version 67 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families