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Protein

Sphingosine 1-phosphate receptor 2

Gene

s1pr2

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P receptor is critical for cell migration and epithlial integrity during vertebrate embryogenesis.

GO - Molecular functioni

GO - Biological processi

  • anterior lateral line neuromast development Source: ZFIN
  • anterior lateral line neuromast hair cell development Source: ZFIN
  • cardioblast migration to the midline involved in heart rudiment formation Source: ZFIN
  • cell migration involved in gastrulation Source: ZFIN
  • cell migration involved in heart development Source: ZFIN
  • cell migration to the midline involved in heart development Source: ZFIN
  • convergent extension Source: ZFIN
  • convergent extension involved in gastrulation Source: ZFIN
  • embryonic heart tube development Source: ZFIN
  • embryonic heart tube morphogenesis Source: ZFIN
  • embryonic viscerocranium morphogenesis Source: ZFIN
  • endoderm development Source: ZFIN
  • heart development Source: ZFIN
  • heart morphogenesis Source: ZFIN
  • heart rudiment formation Source: ZFIN
  • mesodermal cell migration Source: ZFIN
  • negative regulation of cell migration Source: ZFIN
  • otolith development Source: ZFIN
  • posterior lateral line neuromast hair cell development Source: ZFIN
  • regulation of cartilage development Source: ZFIN
  • semicircular canal development Source: ZFIN
  • sphingosine-1-phosphate signaling pathway Source: ZFIN
  • vasculature development Source: ZFIN
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-DRE-418594. G alpha (i) signalling events.
R-DRE-419408. Lysosphingolipid and LPA receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingosine 1-phosphate receptor 2
Short name:
S1P receptor 2
Short name:
S1P2
Alternative name(s):
Sphingosine 1-phosphate receptor Edg-5
Short name:
S1P receptor Edg-5
Gene namesi
Name:s1pr2
Synonyms:edg5
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 3

Organism-specific databases

ZFINiZDB-GENE-020123-2. s1pr2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5757ExtracellularBy similarityAdd
BLAST
Transmembranei58 – 7821Helical; Name=1By similarityAdd
BLAST
Topological domaini79 – 879CytoplasmicBy similarity
Transmembranei88 – 10821Helical; Name=2By similarityAdd
BLAST
Topological domaini109 – 12820ExtracellularBy similarityAdd
BLAST
Transmembranei129 – 14921Helical; Name=3By similarityAdd
BLAST
Topological domaini150 – 16718CytoplasmicBy similarityAdd
BLAST
Transmembranei168 – 19326Helical; Name=4By similarityAdd
BLAST
Topological domaini194 – 21926ExtracellularBy similarityAdd
BLAST
Transmembranei220 – 23011Helical; Name=5By similarityAdd
BLAST
Topological domaini231 – 25424CytoplasmicBy similarityAdd
BLAST
Transmembranei255 – 27521Helical; Name=6By similarityAdd
BLAST
Topological domaini276 – 28914ExtracellularBy similarityAdd
BLAST
Transmembranei290 – 31021Helical; Name=7By similarityAdd
BLAST
Topological domaini311 – 37060CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Involvement in diseasei

Defects in s1pr2 are a cause of heart development abnormality named miles apart (mil). In all vertebrates, the myocardial progenitors involute early during gastrulation and come to occupy bilateral positions in the anterior lateral plate mesoderm (LPM). During somitogenesis, these cells undergo a second phase of migration toward the midline and fuse to form the definitive heart tube. Defects in S1PR2 disrupt this process, leading to the formation of two laterally positioned hearts (cardia bifida). The mil phenotype for which two recessive alleles exist, mil(m93) and mil(te273) are fully penetrant. Mil(m93)/mil(te273) transheterozygous embryos display the same phenotype as homozygotes for either single mutant allele. In addition to cardia bifida, mil mutants display epithelial tail blisters, indicative of a defect in epithelial integrity.

Keywords - Diseasei

Disease mutation

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 370370Sphingosine 1-phosphate receptor 2PRO_0000069430Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi24 – 241N-linked (GlcNAc...)Sequence analysis
Lipidationi325 – 3251S-palmitoyl cysteineBy similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiQ9I8K8.

Expressioni

Developmental stagei

The expression pattern is complex and dynamic. Maternal expression is found in a diffuse pattern throughout the blastoderm, and this pattern persists through the onset of gastrulation. More pronounced expression can be seen at tailbud stage in the anterior portion of the embryo and along the embryonic axis, and at the 16-somite stage in the midbrain/hindbrain boundary and the tip of the tail where blisters later develop in receptor mutants. At the 18-somite stage, expression appears just lateral to the midline, and as the myocardial precursors migrate to the midline, their location overlaps with this domain of receptor expression.

Gene expression databases

BgeeiENSDARG00000036548.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000053105.

Structurei

3D structure databases

ProteinModelPortaliQ9I8K8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHTX. Eukaryota.
ENOG41100F8. LUCA.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000233501.
HOVERGENiHBG103071.
InParanoidiQ9I8K8.
KOiK04292.
OMAiCVCETAT.
OrthoDBiEOG091G0DD4.
PhylomeDBiQ9I8K8.
TreeFamiTF330052.

Family and domain databases

InterProiIPR000987. EDG1_rcpt.
IPR004063. EDG5_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR004061. S1P_rcpt.
[Graphical view]
PANTHERiPTHR22750:SF17. PTHR22750:SF17. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00642. EDG1RECEPTOR.
PR00237. GPCRRHODOPSN.
PR01523. S1PRECEPTOR.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9I8K8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTCRLFAGF CQAVTMSKYS QYFNKTLIQV HYLTAKEMTA EELRDRIESK
60 70 80 90 100
QSLSSLNILF VVICSIIILE NLLVLIAVFR NKKFHSAMFF FIGNLAFSDL
110 120 130 140 150
LAGSAYIANI FLSGPRTFHL TPVQWFIREG TAFIALSASV FSLLAIAIER
160 170 180 190 200
YIAITKVKVY GSNKTCRMFL LIGACWVMSI LLGGLPIIGW NCINNLDDCS
210 220 230 240 250
AVLPLNTRYY IRFVVTIFSI ILLSIVILYV RIYLIVRTSH QEATNSPAYA
260 270 280 290 300
LLKTVTIVLG VFIICWLPAF TILLLDTSCK MKQCPILNNA GIFFSFATLN
310 320 330 340 350
SALNPLIYTL RSKDMRKEFL RVLCCWGLLN CGRPPHRCMV PLKSSSSMEH
360 370
CTNKHEHQSI PIMQDCTTCV
Length:370
Mass (Da):41,777
Last modified:October 1, 2000 - v1
Checksum:i8C6B3A06DEEA6757
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti150 – 1501R → H in mil; allele m93; lack of S1P-mediated signaling. 1 Publication
Natural varianti167 – 1671R → C in mil; allele te273; lack of S1P-mediated signaling. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF260256 mRNA. Translation: AAF88001.1.
RefSeqiNP_001153442.1. NM_001159970.1.
UniGeneiDr.110802.

Genome annotation databases

EnsembliENSDART00000053106; ENSDARP00000053105; ENSDARG00000036548.
GeneIDi170457.
KEGGidre:170457.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF260256 mRNA. Translation: AAF88001.1.
RefSeqiNP_001153442.1. NM_001159970.1.
UniGeneiDr.110802.

3D structure databases

ProteinModelPortaliQ9I8K8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000053105.

Protein family/group databases

GPCRDBiSearch...

Proteomic databases

PaxDbiQ9I8K8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000053106; ENSDARP00000053105; ENSDARG00000036548.
GeneIDi170457.
KEGGidre:170457.

Organism-specific databases

CTDi9294.
ZFINiZDB-GENE-020123-2. s1pr2.

Phylogenomic databases

eggNOGiENOG410IHTX. Eukaryota.
ENOG41100F8. LUCA.
GeneTreeiENSGT00760000118804.
HOGENOMiHOG000233501.
HOVERGENiHBG103071.
InParanoidiQ9I8K8.
KOiK04292.
OMAiCVCETAT.
OrthoDBiEOG091G0DD4.
PhylomeDBiQ9I8K8.
TreeFamiTF330052.

Enzyme and pathway databases

ReactomeiR-DRE-418594. G alpha (i) signalling events.
R-DRE-419408. Lysosphingolipid and LPA receptors.

Miscellaneous databases

PROiQ9I8K8.

Gene expression databases

BgeeiENSDARG00000036548.

Family and domain databases

InterProiIPR000987. EDG1_rcpt.
IPR004063. EDG5_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR004061. S1P_rcpt.
[Graphical view]
PANTHERiPTHR22750:SF17. PTHR22750:SF17. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00642. EDG1RECEPTOR.
PR00237. GPCRRHODOPSN.
PR01523. S1PRECEPTOR.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiS1PR2_DANRE
AccessioniPrimary (citable) accession number: Q9I8K8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 16, 2004
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.