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Q9I7F7

- PR2_DROME

UniProt

Q9I7F7 - PR2_DROME

Protein

Tyrosine-protein kinase PR2

Gene

PR2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 3 (27 Jan 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei164 – 1641ATPPROSITE-ProRule annotation
    Active sitei260 – 2601Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi139 – 1479ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
    3. protein tyrosine kinase activity Source: UniProtKB

    GO - Biological processi

    1. autophagic cell death Source: FlyBase
    2. peptidyl-tyrosine phosphorylation Source: GOC
    3. protein phosphorylation Source: UniProtKB
    4. salivary gland cell autophagic cell death Source: FlyBase

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein kinase PR2 (EC:2.7.10.2)
    Gene namesi
    Name:PR2
    ORF Names:CG3969
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2R

    Organism-specific databases

    FlyBaseiFBgn0013955. PR2.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13371337Tyrosine-protein kinase PR2PRO_0000088132Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei71 – 711Phosphoserine1 Publication
    Modified residuei291 – 2911Phosphotyrosine1 Publication
    Modified residuei292 – 2921Phosphotyrosine1 Publication
    Modified residuei764 – 7641Phosphoserine1 Publication
    Modified residuei778 – 7781Phosphoserine1 Publication
    Modified residuei831 – 8311Phosphoserine1 Publication
    Modified residuei918 – 9181Phosphoserine1 Publication
    Modified residuei924 – 9241Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9I7F7.

    Expressioni

    Gene expression databases

    BgeeiQ9I7F7.

    Interactioni

    Protein-protein interaction databases

    STRINGi7227.FBpp0086889.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9I7F7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini133 – 399267Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini488 – 50215CRIBPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family.PROSITE-ProRule annotation
    Contains 1 CRIB domain.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00740000115195.
    InParanoidiQ9I7F7.
    PhylomeDBiQ9I7F7.

    Family and domain databases

    Gene3Di3.90.810.10. 1 hit.
    InterProiIPR000095. CRIB_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR011511. SH3_2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF00786. PBD. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    PF07653. SH3_2. 1 hit.
    [Graphical view]
    SMARTiSM00285. PBD. 1 hit.
    SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS50108. CRIB. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: Q9I7F7-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEYPQIDLYE FLTESELQQY YNAVKNELKI TNAAQFKYAA DEDLRFIGLS     50
    RPEIRRLRKF YEKHFPHSYL SKIKRLLQAP GTMVKREEAP GGGSQVALDG 100
    SSASACSSLA AKNGASSPSK VPNNKHIIPA DSISVNKQLG TGEFGIVQQG 150
    VWSNGNERIQ VAIKCLCRER MQSNPMEFLK EAAIMHSIEH ENIVRLYGVV 200
    LATDSLMLVT ELAHLRSLLE CLKDSGLRVS FLTIPTLCEF ALQICNGMRY 250
    LEQKRLIHRD LAARNILVFS KDKVKISDFG LSRALGVGKD YYKTNFNVNL 300
    KLPIAWCAPE CINYLRFTNA SDVWAFGVCL WEMFSYGFQP WAALTGLQIL 350
    EAIDAPNYQR LEQPDCCPSE YYTLMMKCWQ DDAAKRPRFG EIYDQLPDMK 400
    PEQLKAVVNC TEPKKDHLLY RQGDIISVLD RNTGTPFWKG VLSTGKTGYF 450
    NPSNTVAFLE GLPSSTRDSF SRVSDHRISK RKLRTEMISK PQNDFKHTGH 500
    VGIDGATFGD IAFLGSSQNY NHVPKQIVTP YKPSEDIEQT PLLLPPTPTS 550
    PDSLQTASGY FPEGANSGGA MGTSMNPTFI PSAEHTPKLI ATNGQSSFDF 600
    ASGSTNPFFP NRGDDELEFG LHNYGADGKS VHSETGWRPT SRSIVDDPHE 650
    YHEISDDEIA ADKLDFGPSL LDEINSMFGS ISAATGSHPK SPGFDHVNNK 700
    NEITEMSAKL GQKSGDTNGN KHGHGLLPTL SKKKSSGTVK PISVKDEKIL 750
    NHAIEIANEI SARSMIDLVS DQTPVIHSPK RKFSFRFPHL SNNGSGDKSG 800
    GLGTSGSAHT PTHGNASPFP KKKNFTEELQ SIPDIQSLIG KEGLEAYNSL 850
    IERKALLDIG PSPAATLLRH LDTDEFDLQS LHQSQRPMTL PTRGATQRVR 900
    KAELAAGLSR HNDENSNSLE ACESPSYMTH GSYKFPEAQP TEQLPEPESP 950
    NPIPLPPREG KKQVKTSTKR HVRKYPLIIP ANGLQRTLSK LADFGDEAAK 1000
    SPEISTSSQP QPGRAIEVVA AVRPSGMRRP SRPSEREYEN MPTVGKESAH 1050
    TYQNLDKLTP ADAAGLTDTA SLQFESIMEA DTSKEGILQS PDVTDGFYNF 1100
    SIQKEHYNKG KDAEFEATQI SGLYVNDDEL RNLDIESSRR TATPCSSCSA 1150
    LESEHSQPDA LPSTESVSEV SRFSSVDNEL AGNALFKKVR ASVNMAMNRK 1200
    SVAETSLTSN QPGGASAKPQ TEAEYFAATA ARLADSNSVS CEDLLEFSDK 1250
    KPKGCERGVD SDEVRIMVKV LGKDSTPNRC LGALEFINWD VHKSIKLIKL 1300
    QNLVSEANLS LEASFEALQQ HEWDLHTTAH KLNGLKL 1337
    Length:1,337
    Mass (Da):147,477
    Last modified:January 27, 2003 - v3
    Checksum:i12650E9DF5A82724
    GO
    Isoform Short (identifier: Q9I7F7-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-82: Missing.

    Show »
    Length:1,255
    Mass (Da):137,621
    Checksum:iAB0C0C1FF2E2816B
    GO

    Sequence cautioni

    The sequence BAA04489.1 differs from that shown. Reason: Frameshift at positions 752, 806, 816 and 827.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti450 – 4501F → FSTPFWKGVLSTGKTGYF(PubMed:8112607)Curated
    Sequence conflicti484 – 4841R → A in BAA04489. (PubMed:8112607)Curated
    Sequence conflicti906 – 9061A → P(PubMed:8112607)Curated
    Sequence conflicti913 – 9131D → V(PubMed:8112607)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 8282Missing in isoform Short. CuratedVSP_005006Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE013599 Genomic DNA. Translation: AAF58423.4.
    AE013599 Genomic DNA. Translation: AAG22275.3.
    AY051755 mRNA. Translation: AAK93179.1.
    D17551 mRNA. Translation: BAA04489.1. Frameshift.
    AJ002909 Genomic DNA. Translation: CAA05744.1.
    RefSeqiNP_477086.2. NM_057738.4. [Q9I7F7-1]
    NP_477087.3. NM_057739.4.
    UniGeneiDm.4488.

    Genome annotation databases

    EnsemblMetazoaiFBtr0330329; FBpp0303361; FBgn0263998. [Q9I7F7-1]
    GeneIDi36442.
    KEGGidme:Dmel_CG43741.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE013599 Genomic DNA. Translation: AAF58423.4 .
    AE013599 Genomic DNA. Translation: AAG22275.3 .
    AY051755 mRNA. Translation: AAK93179.1 .
    D17551 mRNA. Translation: BAA04489.1 . Frameshift.
    AJ002909 Genomic DNA. Translation: CAA05744.1 .
    RefSeqi NP_477086.2. NM_057738.4. [Q9I7F7-1 ]
    NP_477087.3. NM_057739.4.
    UniGenei Dm.4488.

    3D structure databases

    ProteinModelPortali Q9I7F7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7227.FBpp0086889.

    Proteomic databases

    PaxDbi Q9I7F7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0330329 ; FBpp0303361 ; FBgn0263998 . [Q9I7F7-1 ]
    GeneIDi 36442.
    KEGGi dme:Dmel_CG43741.

    Organism-specific databases

    CTDi 36442.
    FlyBasei FBgn0013955. PR2.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00740000115195.
    InParanoidi Q9I7F7.
    PhylomeDBi Q9I7F7.

    Miscellaneous databases

    ChiTaRSi PR2. drosophila.
    GenomeRNAii 36442.
    NextBioi 798590.

    Gene expression databases

    Bgeei Q9I7F7.

    Family and domain databases

    Gene3Di 3.90.810.10. 1 hit.
    InterProi IPR000095. CRIB_dom.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR011511. SH3_2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF00786. PBD. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    PF07653. SH3_2. 1 hit.
    [Graphical view ]
    SMARTi SM00285. PBD. 1 hit.
    SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS50108. CRIB. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    2. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
      Strain: Berkeley.
      Tissue: Embryo.
    4. "Alternative splicing generates two distinct transcripts for the Drosophila melanogaster fibroblast growth factor receptor homolog."
      Ito M., Matsui T., Taniguchi T., Chihara K.
      Gene 139:215-218(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-917 (ISOFORM LONG).
      Strain: Oregon-R.
      Tissue: Embryo.
    5. "Sampling the genomic pool of protein tyrosine kinase genes using the polymerase chain reaction with genomic DNA."
      Oates A.C., Wollberg P., Achen M.G., Wilks A.F.
      Biochem. Biophys. Res. Commun. 249:660-667(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 266-321.
    6. "An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells."
      Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A.
      Mol. Biosyst. 3:275-286(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-831, IDENTIFICATION BY MASS SPECTROMETRY.
    7. "Phosphoproteome analysis of Drosophila melanogaster embryos."
      Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
      J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-71; TYR-291; TYR-292; SER-764; SER-778; SER-918 AND SER-924, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Embryo.

    Entry informationi

    Entry nameiPR2_DROME
    AccessioniPrimary (citable) accession number: Q9I7F7
    Secondary accession number(s): Q24316, Q960Z6, Q9V6K0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 27, 2003
    Last sequence update: January 27, 2003
    Last modified: October 1, 2014
    This is version 112 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3