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Q9I7F7 (PR2_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosine-protein kinase PR2

EC=2.7.10.2
Gene names
Name:PR2
ORF Names:CG3969
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length1337 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family.

Contains 1 CRIB domain.

Contains 1 protein kinase domain.

Sequence caution

The sequence BAA04489.1 differs from that shown. Reason: Frameshift at positions 752, 806, 816 and 827.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: Q9I7F7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: Q9I7F7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-82: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 13371337Tyrosine-protein kinase PR2
PRO_0000088132

Regions

Domain133 – 399267Protein kinase
Domain488 – 50215CRIB
Nucleotide binding139 – 1479ATP By similarity

Sites

Active site2601Proton acceptor By similarity
Binding site1641ATP By similarity

Amino acid modifications

Modified residue711Phosphoserine Ref.7
Modified residue2911Phosphotyrosine Ref.7
Modified residue2921Phosphotyrosine Ref.7
Modified residue7641Phosphoserine Ref.7
Modified residue7781Phosphoserine Ref.7
Modified residue8311Phosphoserine Ref.6
Modified residue9181Phosphoserine Ref.7
Modified residue9241Phosphoserine Ref.7

Natural variations

Alternative sequence1 – 8282Missing in isoform Short.
VSP_005006

Experimental info

Sequence conflict4501F → FSTPFWKGVLSTGKTGYF Ref.4
Sequence conflict4841R → A in BAA04489. Ref.4
Sequence conflict9061A → P Ref.4
Sequence conflict9131D → V Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified January 27, 2003. Version 3.
Checksum: 12650E9DF5A82724

FASTA1,337147,477
        10         20         30         40         50         60 
MEYPQIDLYE FLTESELQQY YNAVKNELKI TNAAQFKYAA DEDLRFIGLS RPEIRRLRKF 

        70         80         90        100        110        120 
YEKHFPHSYL SKIKRLLQAP GTMVKREEAP GGGSQVALDG SSASACSSLA AKNGASSPSK 

       130        140        150        160        170        180 
VPNNKHIIPA DSISVNKQLG TGEFGIVQQG VWSNGNERIQ VAIKCLCRER MQSNPMEFLK 

       190        200        210        220        230        240 
EAAIMHSIEH ENIVRLYGVV LATDSLMLVT ELAHLRSLLE CLKDSGLRVS FLTIPTLCEF 

       250        260        270        280        290        300 
ALQICNGMRY LEQKRLIHRD LAARNILVFS KDKVKISDFG LSRALGVGKD YYKTNFNVNL 

       310        320        330        340        350        360 
KLPIAWCAPE CINYLRFTNA SDVWAFGVCL WEMFSYGFQP WAALTGLQIL EAIDAPNYQR 

       370        380        390        400        410        420 
LEQPDCCPSE YYTLMMKCWQ DDAAKRPRFG EIYDQLPDMK PEQLKAVVNC TEPKKDHLLY 

       430        440        450        460        470        480 
RQGDIISVLD RNTGTPFWKG VLSTGKTGYF NPSNTVAFLE GLPSSTRDSF SRVSDHRISK 

       490        500        510        520        530        540 
RKLRTEMISK PQNDFKHTGH VGIDGATFGD IAFLGSSQNY NHVPKQIVTP YKPSEDIEQT 

       550        560        570        580        590        600 
PLLLPPTPTS PDSLQTASGY FPEGANSGGA MGTSMNPTFI PSAEHTPKLI ATNGQSSFDF 

       610        620        630        640        650        660 
ASGSTNPFFP NRGDDELEFG LHNYGADGKS VHSETGWRPT SRSIVDDPHE YHEISDDEIA 

       670        680        690        700        710        720 
ADKLDFGPSL LDEINSMFGS ISAATGSHPK SPGFDHVNNK NEITEMSAKL GQKSGDTNGN 

       730        740        750        760        770        780 
KHGHGLLPTL SKKKSSGTVK PISVKDEKIL NHAIEIANEI SARSMIDLVS DQTPVIHSPK 

       790        800        810        820        830        840 
RKFSFRFPHL SNNGSGDKSG GLGTSGSAHT PTHGNASPFP KKKNFTEELQ SIPDIQSLIG 

       850        860        870        880        890        900 
KEGLEAYNSL IERKALLDIG PSPAATLLRH LDTDEFDLQS LHQSQRPMTL PTRGATQRVR 

       910        920        930        940        950        960 
KAELAAGLSR HNDENSNSLE ACESPSYMTH GSYKFPEAQP TEQLPEPESP NPIPLPPREG 

       970        980        990       1000       1010       1020 
KKQVKTSTKR HVRKYPLIIP ANGLQRTLSK LADFGDEAAK SPEISTSSQP QPGRAIEVVA 

      1030       1040       1050       1060       1070       1080 
AVRPSGMRRP SRPSEREYEN MPTVGKESAH TYQNLDKLTP ADAAGLTDTA SLQFESIMEA 

      1090       1100       1110       1120       1130       1140 
DTSKEGILQS PDVTDGFYNF SIQKEHYNKG KDAEFEATQI SGLYVNDDEL RNLDIESSRR 

      1150       1160       1170       1180       1190       1200 
TATPCSSCSA LESEHSQPDA LPSTESVSEV SRFSSVDNEL AGNALFKKVR ASVNMAMNRK 

      1210       1220       1230       1240       1250       1260 
SVAETSLTSN QPGGASAKPQ TEAEYFAATA ARLADSNSVS CEDLLEFSDK KPKGCERGVD 

      1270       1280       1290       1300       1310       1320 
SDEVRIMVKV LGKDSTPNRC LGALEFINWD VHKSIKLIKL QNLVSEANLS LEASFEALQQ 

      1330 
HEWDLHTTAH KLNGLKL 

« Hide

Isoform Short [UniParc].

Checksum: AB0C0C1FF2E2816B
Show »

FASTA1,255137,621

References

« Hide 'large scale' references
[1]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[2]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[3]"A Drosophila full-length cDNA resource."
Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
Strain: Berkeley.
Tissue: Embryo.
[4]"Alternative splicing generates two distinct transcripts for the Drosophila melanogaster fibroblast growth factor receptor homolog."
Ito M., Matsui T., Taniguchi T., Chihara K.
Gene 139:215-218(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-917 (ISOFORM LONG).
Strain: Oregon-R.
Tissue: Embryo.
[5]"Sampling the genomic pool of protein tyrosine kinase genes using the polymerase chain reaction with genomic DNA."
Oates A.C., Wollberg P., Achen M.G., Wilks A.F.
Biochem. Biophys. Res. Commun. 249:660-667(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 266-321.
[6]"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells."
Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A.
Mol. Biosyst. 3:275-286(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-831, IDENTIFICATION BY MASS SPECTROMETRY.
[7]"Phosphoproteome analysis of Drosophila melanogaster embryos."
Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-71; TYR-291; TYR-292; SER-764; SER-778; SER-918 AND SER-924, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Embryo.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE013599 Genomic DNA. Translation: AAF58423.4.
AE013599 Genomic DNA. Translation: AAG22275.3.
AY051755 mRNA. Translation: AAK93179.1.
D17551 mRNA. Translation: BAA04489.1. Frameshift.
AJ002909 Genomic DNA. Translation: CAA05744.1.
RefSeqNP_477086.2. NM_057738.4.
NP_477087.3. NM_057739.4.
UniGeneDm.4488.

3D structure databases

ProteinModelPortalQ9I7F7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7227.FBpp0086889.

Proteomic databases

PaxDbQ9I7F7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0330329; FBpp0303361; FBgn0263998. [Q9I7F7-1]
GeneID36442.
KEGGdme:Dmel_CG43741.

Organism-specific databases

CTD36442.
FlyBaseFBgn0013955. PR2.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00740000115195.
InParanoidQ9I7F7.
PhylomeDBQ9I7F7.

Gene expression databases

BgeeQ9I7F7.

Family and domain databases

Gene3D3.90.810.10. 1 hit.
InterProIPR000095. CRIB_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR011511. SH3_2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamPF00786. PBD. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF07653. SH3_2. 1 hit.
[Graphical view]
SMARTSM00285. PBD. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS50108. CRIB. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPR2. drosophila.
GenomeRNAi36442.
NextBio798590.

Entry information

Entry namePR2_DROME
AccessionPrimary (citable) accession number: Q9I7F7
Secondary accession number(s): Q24316, Q960Z6, Q9V6K0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: January 27, 2003
Last modified: April 16, 2014
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase