Q9I7F7 (PR2_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase PR2
EC=2.7.10.2

Gene names

Name:	PR2
ORF Names:	CG3969

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	1337 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. Contains 1 CRIB domain. Contains 1 protein kinase domain.
Sequence caution	The sequence BAA04489.1 differs from that shown. Reason: Frameshift at positions 752, 806, 816 and 827.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	salivary gland cell autophagic cell death Inferred from expression pattern PubMed 12593804. Source: FlyBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein tyrosine kinase activity Non-traceable author statement Ref.5. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: Q9I7F7-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: Q9I7F7-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-82: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1337	1337	Tyrosine-protein kinase PR2		PRO_0000088132
Regions
	Domain	133 – 399	267	Protein kinase
	Domain	488 – 502	15	CRIB
	Nucleotide binding	139 – 147	9	ATP By similarity
Sites
	Active site	260	1	Proton acceptor By similarity
	Binding site	164	1	ATP By similarity
Amino acid modifications
	Modified residue	71	1	Phosphoserine Ref.7
	Modified residue	291	1	Phosphotyrosine Ref.7
	Modified residue	292	1	Phosphotyrosine Ref.7
	Modified residue	764	1	Phosphoserine Ref.7
	Modified residue	778	1	Phosphoserine Ref.7
	Modified residue	831	1	Phosphoserine Ref.6
	Modified residue	918	1	Phosphoserine Ref.7
	Modified residue	924	1	Phosphoserine Ref.7
Natural variations
	Alternative sequence	1 – 82	82	Missing in isoform Short.		VSP_005006
Experimental info
	Sequence conflict	450	1	F → FSTPFWKGVLSTGKTGYF Ref.4
	Sequence conflict	484	1	R → A in BAA04489. Ref.4
	Sequence conflict	906	1	A → P Ref.4
	Sequence conflict	913	1	D → V Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified January 27, 2003. Version 3. Checksum: 12650E9DF5A82724	FASTA	1,337	147,477
	10 20 30 40 50 60 MEYPQIDLYE FLTESELQQY YNAVKNELKI TNAAQFKYAA DEDLRFIGLS RPEIRRLRKF 70 80 90 100 110 120 YEKHFPHSYL SKIKRLLQAP GTMVKREEAP GGGSQVALDG SSASACSSLA AKNGASSPSK 130 140 150 160 170 180 VPNNKHIIPA DSISVNKQLG TGEFGIVQQG VWSNGNERIQ VAIKCLCRER MQSNPMEFLK 190 200 210 220 230 240 EAAIMHSIEH ENIVRLYGVV LATDSLMLVT ELAHLRSLLE CLKDSGLRVS FLTIPTLCEF 250 260 270 280 290 300 ALQICNGMRY LEQKRLIHRD LAARNILVFS KDKVKISDFG LSRALGVGKD YYKTNFNVNL 310 320 330 340 350 360 KLPIAWCAPE CINYLRFTNA SDVWAFGVCL WEMFSYGFQP WAALTGLQIL EAIDAPNYQR 370 380 390 400 410 420 LEQPDCCPSE YYTLMMKCWQ DDAAKRPRFG EIYDQLPDMK PEQLKAVVNC TEPKKDHLLY 430 440 450 460 470 480 RQGDIISVLD RNTGTPFWKG VLSTGKTGYF NPSNTVAFLE GLPSSTRDSF SRVSDHRISK 490 500 510 520 530 540 RKLRTEMISK PQNDFKHTGH VGIDGATFGD IAFLGSSQNY NHVPKQIVTP YKPSEDIEQT 550 560 570 580 590 600 PLLLPPTPTS PDSLQTASGY FPEGANSGGA MGTSMNPTFI PSAEHTPKLI ATNGQSSFDF 610 620 630 640 650 660 ASGSTNPFFP NRGDDELEFG LHNYGADGKS VHSETGWRPT SRSIVDDPHE YHEISDDEIA 670 680 690 700 710 720 ADKLDFGPSL LDEINSMFGS ISAATGSHPK SPGFDHVNNK NEITEMSAKL GQKSGDTNGN 730 740 750 760 770 780 KHGHGLLPTL SKKKSSGTVK PISVKDEKIL NHAIEIANEI SARSMIDLVS DQTPVIHSPK 790 800 810 820 830 840 RKFSFRFPHL SNNGSGDKSG GLGTSGSAHT PTHGNASPFP KKKNFTEELQ SIPDIQSLIG 850 860 870 880 890 900 KEGLEAYNSL IERKALLDIG PSPAATLLRH LDTDEFDLQS LHQSQRPMTL PTRGATQRVR 910 920 930 940 950 960 KAELAAGLSR HNDENSNSLE ACESPSYMTH GSYKFPEAQP TEQLPEPESP NPIPLPPREG 970 980 990 1000 1010 1020 KKQVKTSTKR HVRKYPLIIP ANGLQRTLSK LADFGDEAAK SPEISTSSQP QPGRAIEVVA 1030 1040 1050 1060 1070 1080 AVRPSGMRRP SRPSEREYEN MPTVGKESAH TYQNLDKLTP ADAAGLTDTA SLQFESIMEA 1090 1100 1110 1120 1130 1140 DTSKEGILQS PDVTDGFYNF SIQKEHYNKG KDAEFEATQI SGLYVNDDEL RNLDIESSRR 1150 1160 1170 1180 1190 1200 TATPCSSCSA LESEHSQPDA LPSTESVSEV SRFSSVDNEL AGNALFKKVR ASVNMAMNRK 1210 1220 1230 1240 1250 1260 SVAETSLTSN QPGGASAKPQ TEAEYFAATA ARLADSNSVS CEDLLEFSDK KPKGCERGVD 1270 1280 1290 1300 1310 1320 SDEVRIMVKV LGKDSTPNRC LGALEFINWD VHKSIKLIKL QNLVSEANLS LEASFEALQQ 1330 HEWDLHTTAH KLNGLKL
	Isoform Short [UniParc]. Checksum: AB0C0C1FF2E2816B	FASTA	1,255	137,621
	10 20 30 40 50 60 MVKREEAPGG GSQVALDGSS ASACSSLAAK NGASSPSKVP NNKHIIPADS ISVNKQLGTG 70 80 90 100 110 120 EFGIVQQGVW SNGNERIQVA IKCLCRERMQ SNPMEFLKEA AIMHSIEHEN IVRLYGVVLA 130 140 150 160 170 180 TDSLMLVTEL AHLRSLLECL KDSGLRVSFL TIPTLCEFAL QICNGMRYLE QKRLIHRDLA 190 200 210 220 230 240 ARNILVFSKD KVKISDFGLS RALGVGKDYY KTNFNVNLKL PIAWCAPECI NYLRFTNASD 250 260 270 280 290 300 VWAFGVCLWE MFSYGFQPWA ALTGLQILEA IDAPNYQRLE QPDCCPSEYY TLMMKCWQDD 310 320 330 340 350 360 AAKRPRFGEI YDQLPDMKPE QLKAVVNCTE PKKDHLLYRQ GDIISVLDRN TGTPFWKGVL 370 380 390 400 410 420 STGKTGYFNP SNTVAFLEGL PSSTRDSFSR VSDHRISKRK LRTEMISKPQ NDFKHTGHVG 430 440 450 460 470 480 IDGATFGDIA FLGSSQNYNH VPKQIVTPYK PSEDIEQTPL LLPPTPTSPD SLQTASGYFP 490 500 510 520 530 540 EGANSGGAMG TSMNPTFIPS AEHTPKLIAT NGQSSFDFAS GSTNPFFPNR GDDELEFGLH 550 560 570 580 590 600 NYGADGKSVH SETGWRPTSR SIVDDPHEYH EISDDEIAAD KLDFGPSLLD EINSMFGSIS 610 620 630 640 650 660 AATGSHPKSP GFDHVNNKNE ITEMSAKLGQ KSGDTNGNKH GHGLLPTLSK KKSSGTVKPI 670 680 690 700 710 720 SVKDEKILNH AIEIANEISA RSMIDLVSDQ TPVIHSPKRK FSFRFPHLSN NGSGDKSGGL 730 740 750 760 770 780 GTSGSAHTPT HGNASPFPKK KNFTEELQSI PDIQSLIGKE GLEAYNSLIE RKALLDIGPS 790 800 810 820 830 840 PAATLLRHLD TDEFDLQSLH QSQRPMTLPT RGATQRVRKA ELAAGLSRHN DENSNSLEAC 850 860 870 880 890 900 ESPSYMTHGS YKFPEAQPTE QLPEPESPNP IPLPPREGKK QVKTSTKRHV RKYPLIIPAN 910 920 930 940 950 960 GLQRTLSKLA DFGDEAAKSP EISTSSQPQP GRAIEVVAAV RPSGMRRPSR PSEREYENMP 970 980 990 1000 1010 1020 TVGKESAHTY QNLDKLTPAD AAGLTDTASL QFESIMEADT SKEGILQSPD VTDGFYNFSI 1030 1040 1050 1060 1070 1080 QKEHYNKGKD AEFEATQISG LYVNDDELRN LDIESSRRTA TPCSSCSALE SEHSQPDALP 1090 1100 1110 1120 1130 1140 STESVSEVSR FSSVDNELAG NALFKKVRAS VNMAMNRKSV AETSLTSNQP GGASAKPQTE 1150 1160 1170 1180 1190 1200 AEYFAATAAR LADSNSVSCE DLLEFSDKKP KGCERGVDSD EVRIMVKVLG KDSTPNRCLG 1210 1220 1230 1240 1250 ALEFINWDVH KSIKLIKLQN LVSEANLSLE ASFEALQQHE WDLHTTAHKL NGLKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG). Strain: Berkeley. Tissue: Embryo.
[4]	"Alternative splicing generates two distinct transcripts for the Drosophila melanogaster fibroblast growth factor receptor homolog." Ito M., Matsui T., Taniguchi T., Chihara K. Gene 139:215-218(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-917 (ISOFORM LONG). Strain: Oregon-R. Tissue: Embryo.
[5]	"Sampling the genomic pool of protein tyrosine kinase genes using the polymerase chain reaction with genomic DNA." Oates A.C., Wollberg P., Achen M.G., Wilks A.F. Biochem. Biophys. Res. Commun. 249:660-667(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 266-321.
[6]	"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells." Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A. Mol. Biosyst. 3:275-286(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-831, MASS SPECTROMETRY.
[7]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-71; TYR-291; TYR-292; SER-764; SER-778; SER-918 AND SER-924, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013599 Genomic DNA. Translation: AAF58423.4. AE013599 Genomic DNA. Translation: AAG22275.3. AY051755 mRNA. Translation: AAK93179.1. D17551 mRNA. Translation: BAA04489.1. Frameshift. AJ002909 Genomic DNA. Translation: CAA05744.1.
RefSeq	NP_477086.2. NM_057738.4. NP_477087.3. NM_057739.4.
UniGene	Dm.4488.
3D structure databases
ProteinModelPortal	Q9I7F7.
SMR	Q9I7F7. Positions 92-437.
ModBase	Search...
Proteomic databases
PaxDb	Q9I7F7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0330329; FBpp0303361; FBgn0263998.
GeneID	36442.
KEGG	dme:Dmel_CG3969.
Organism-specific databases
CTD	36442.
FlyBase	FBgn0013955. PR2.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00630000089536.
InParanoid	Q9I7F7.
KO	K08252.
OMA	LDRNTGT.
OrthoDB	EOG41NS1Z.
PhylomeDB	Q9I7F7.
Gene expression databases
Bgee	Q9I7F7.
GermOnline	CG3969. Drosophila melanogaster.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000095. PAK_box_Rho-bd. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR001452. SH3_domain. IPR008266. Tyr_kinase_AS. IPR020635. Tyr_kinase_cat_dom. [Graphical view]
Pfam	PF00786. PBD. 1 hit. PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
SMART	SM00285. PBD. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS50108. CRIB. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PR2. drosophila.
GenomeRNAi	36442.
NextBio	798590.

Entry information

Entry name

PR2_DROME

Accession

Primary (citable) accession number: Q9I7F7
Secondary accession number(s): Q24316, Q960Z6, Q9V6K0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 27, 2003
Last sequence update:	January 27, 2003
Last modified:	May 1, 2013
This is version 101 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project