Q9I7B8 (SYGB_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase beta subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase beta subunit
Short name=GlyRS

Gene names

Name:	glyS
Ordered Locus Names:	PA0008

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	684 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP MF_00255
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm HAMAP MF_00255.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW arginine-tRNA ligase activity Inferred from electronic annotation. Source: InterPro glycine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 684	684	Glycine--tRNA ligase beta subunit HAMAP MF_00255		PRO_0000072918

Sequences

	Sequence		Length	Mass (Da)
	Q9I7B8 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 74144075010F0BE6	FASTA	684	73,974
	10 20 30 40 50 60 MSAKDFLVEL GTEELPPKAL NSLGEAFLSG IEKGLKAAGL SYAAARFYAA PRRLAVLVEQ 70 80 90 100 110 120 LAVQQPDRTV NLDGPPLQAA FDASGNPTQA ALGFAKKCGV DLQQIDKSGP KLRFIQTIAG 130 140 150 160 170 180 QPAAGLLPGI VEASLNELPI PKRMRWAARR EEFVRPTQWL VMLFGDDVVE CEILAQKAGR 190 200 210 220 230 240 ESRGHRFHNP DNVRISSPAA YLEDLRGAHV LADFAERREL IAKRVAELAA EQQGSAIVPP 250 260 270 280 290 300 SLLDEVTALV EWPVPLVCSF EERFLEVPQE ALITTMQDNQ KYFCLLDANG KLLPRFITVA 310 320 330 340 350 360 NVESKAPENI VSGNEKVVRP RLTDAEFFFK QDKKQPLESF NERLRNVVFQ AQLGTVFEKA 370 380 390 400 410 420 QRVSGLAAYI AERIGGNAQN ASRAGILSKC DLATEMVGEF PEMQGIAGYY YATHGGEAED 430 440 450 460 470 480 VALALNEQYM PRGAGAELPS TLTGAAVAVA DKLDTLVGIF GIGMLPTGSK DPYALRRAAL 490 500 510 520 530 540 GVLRILIEKQ LDLDLVAAVN AAVEQYGDKV KAAGLAEQVL DFVFDRLRAR YEDEGVDVAV 550 560 570 580 590 600 YQSVRALKPS SPLDFDQRVQ AVQAFRQLPE AEALAAANKR VSNILAKSED EVPPNVDASL 610 620 630 640 650 660 LVEAAEKALG SAVANAESEV APLAAARDYR AALARLAALR EPVDTFFADV MVNVDDAAVR 670 680 ANRYALLAKL RGSFLGVADI SLLG

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG03398.1.
PIR	D83645.
RefSeq	NP_064728.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9I7B8.
ModBase	Search...
Proteomic databases
PRIDE	Q9I7B8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	878214.
GenomeReviews	Gene locus PA0008 in contig AE004091_GR.
KEGG	pae:PA0008.
NMPDR	fig|208964.1.peg.8.
PATRIC	19834212. VBIPseAer58763_0008.
Organism-specific databases
PseudoCAP	PA0008.
Phylogenomic databases
HOGENOM	HBG586599.
OMA	FIRPVHT.
ProtClustDB	PRK01233.
Enzyme and pathway databases
BioCyc	PAER208964:PA0008-MONOMER.
Family and domain databases
HAMAP	MF_00255. Gly_tRNA_synth_beta. [Tree]
InterPro	IPR008909. DALR_anticod-bd. IPR006194. Gly-tRNA-synth_II_heterodimer. IPR015944. Gly-tRNA-synth_IIc_bsu. IPR023279. HD. [Graphical view]
Gene3D	G3DSA:1.10.3210.10. HD. 1 hit.
KO	K01879.
Pfam	PF05746. DALR_1. 1 hit. PF02092. tRNA_synt_2f. 1 hit. [Graphical view]
PRINTS	PR01045. TRNASYNTHGB.
SMART	SM00836. DALR_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00211. GlyS. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGB_PSEAE

Accession

Primary (citable) accession number: Q9I7B8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program