Q9I7B7 (SYGA_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase alpha subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase alpha subunit
Short name=GlyRS

Gene names

Name:	glyQ
Ordered Locus Names:	PA0009

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP MF_00254
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm HAMAP MF_00254.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Glycine--tRNA ligase alpha subunit HAMAP MF_00254		PRO_0000072854

Sequences

	Sequence		Length	Mass (Da)
	Q9I7B7 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: C13AB05877BAD116	FASTA	315	36,141
	10 20 30 40 50 60 MSQTTPAVRT FQDLILALQN YWAEQGCVVL QPYDMEVGAG TFHTATFLRA IGPETWNAAY 70 80 90 100 110 120 VQPSRRPTDG RYGENPNRLQ HYYQFQVVLK PNPENFQELY LGSLKAIGID PLVHDIRFVE 130 140 150 160 170 180 DNWESPTLGA WGLGWEIWLN GMEVTQFTYF QQVGGIECYP VTGEITYGLE RLAMYLQGVD 190 200 210 220 230 240 SVYDLVWTDG PFGKVTYGDV FHQNEVEQST FNFEHANVPK LFELFDFYES EANRLIALEL 250 260 270 280 290 300 PLPTYEMVLK ASHTFNLLDA RRAISVTERQ RYILRVRTLA RAVAQSYLQA RARLGFPMAT 310 PELRDEVLAK LKEAE

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG03399.1.
PIR	E83645.
RefSeq	NP_064729.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9I7B7.
SMR	Q9I7B7. Positions 11-292.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	878095.
GenomeReviews	Gene locus PA0009 in contig AE004091_GR.
KEGG	pae:PA0009.
PATRIC	19834214. VBIPseAer58763_0009.
Organism-specific databases
PseudoCAP	PA0009.
Phylogenomic databases
HOGENOM	HBG285316.
OMA	CRRPTDG.
ProtClustDB	PRK09348.
Enzyme and pathway databases
BioCyc	PAER208964:PA0009-MONOMER.
Family and domain databases
HAMAP	MF_00254. Gly_tRNA_synth_alpha. [Tree]
InterPro	IPR006194. Gly-tRNA-synth_II_heterodimer. IPR002310. Gly-tRNA_synth_IIc_asu. [Graphical view]
KO	K01878.
Pfam	PF02091. tRNA-synt_2e. 1 hit. [Graphical view]
PRINTS	PR01044. TRNASYNTHGA.
TIGRFAMs	TIGR00388. GlyQ. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGA_PSEAE

Accession

Primary (citable) accession number: Q9I7B7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program