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Reviewed, UniProtKB/Swiss-Prot Q9I7A8 (DEF_PSEAE)

Last modified June 16, 2009. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Peptide deformylase
      Short name=PDF
    EC=3.5.1.88
Alternative name(s):
    Polypeptide deformylase
Gene names
Name: def
Ordered Locus Names: PA0019
OrganismPseudomonas aeruginosa [Complete proteome] [HAMAP]
Taxonomic identifier287 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

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Protein attributes

Sequence length168 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity.

Catalytic activity

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163

Cofactor

Binds 1 Fe2+ ion By similarity.

Sequence similarities

Belongs to the polypeptide deformylase family.

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Ontologies

Keywords
   Biological processProtein biosynthesis
   LigandIron
Metal-binding
   Molecular functionHydrolase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processtranslation

Inferred from electronic annotation. Source: HAMAP

   Molecular functioniron ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

peptide deformylase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 168168Peptide deformylase HAMAP MF_00163
PRO_0000082819

Sites

Active site1351 By similarity
Metal binding921Iron By similarity
Metal binding1341Iron By similarity
Metal binding1381Iron By similarity

Secondary structure

1 ........................ 168
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Q9I7A8-1 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: A95F6B921E5F3189

FASTA16819,365
        10         20         30         40         50         60 
MAILNILEFP DPRLRTIAKP VEVVDDAVRQ LIDDMFETMY EAPGIGLAAT QVNVHKRIVV 

        70         80         90        100        110        120 
MDLSEDKSEP RVFINPEFEP LTEDMDQYQE GCLSVPGFYE NVDRPQKVRI KALDRDGNPF 

       130        140        150        160 
EEVAEGLLAV CIQHECDHLN GKLFVDYLST LKRDRIRKKL EKQHRQQA

« Hide

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Cross-references

Sequence databases

AE004091 Genomic DNA. Translation: AAG03409.1.
PIRH83643.
RefSeqNP_248709.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1IX1X-ray1.85A/B1-168[»]
1LRYX-ray2.60A2-168[»]
1N5NX-ray1.80A/B1-168[»]
1S17X-ray1.95A/B1-168[»]
ModBaseSearch...

Genome annotation databases

GeneID879306.
GenomeReviewsGene locus PA0019 in contig AE004091_GR.
KEGGpae:PA0019.

Organism-specific databases

PseudoCAPPA0019.
CMRSearch...

Phylogenomic databases

HOGENOMQ9I7A8.
OMAQ9I7A8. CIQHECD.

Enzyme and pathway databases

BioCycPAER208964:PA0019-MON.
BRENDA3.5.1.88. 354.

Family and domain databases

HAMAPMF_00163.
[Tree]
InterProIPR000181. Fmet_deformylase.
[Graphical view]
Gene3DG3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
PANTHERPTHR10458. Fmet_deformylase. 1 hit.
PfamPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFPIRSF004749. Pep_def. 1 hit.
PRINTSPR01576. PDEFORMYLASE.
ProDomPD003844. Fmet_deformylase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR00079. pept_deformyl. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameDEF_PSEAE
AccessionPrimary (citable) accession number: Q9I7A8
Entry history
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: March 1, 2001
Last modified: June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents