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Q9I7A8

- DEF_PSEAE

UniProt

Q9I7A8 - DEF_PSEAE

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Protein
Peptide deformylase
Gene
def, PA0019
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity.UniRule annotation

Catalytic activityi

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide.UniRule annotation

Cofactori

Binds 1 Fe2+ ion By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi92 – 921Iron By similarity
Metal bindingi134 – 1341Iron By similarity
Active sitei135 – 1351 By similarity
Metal bindingi138 – 1381Iron By similarity

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. peptide deformylase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide deformylase (EC:3.5.1.88)
Short name:
PDF
Alternative name(s):
Polypeptide deformylase
Gene namesi
Name:def
Ordered Locus Names:PA0019
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA0019.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 168168Peptide deformylaseUniRule annotation
PRO_0000082819Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi208964.PA0019.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 154
Helixi26 – 4116
Beta strandi45 – 484
Helixi49 – 524
Beta strandi56 – 627
Beta strandi64 – 674
Beta strandi70 – 8112
Beta strandi85 – 906
Beta strandi100 – 1067
Beta strandi108 – 1136
Beta strandi119 – 1246
Helixi126 – 13914
Helixi144 – 1474
Helixi150 – 16617

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IX1X-ray1.85A/B1-168[»]
1LRYX-ray2.60A2-168[»]
1N5NX-ray1.80A/B1-168[»]
1S17X-ray1.95A/B1-168[»]
ProteinModelPortaliQ9I7A8.
SMRiQ9I7A8. Positions 2-168.

Miscellaneous databases

EvolutionaryTraceiQ9I7A8.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0242.
KOiK01462.
OMAiDMYDTMD.
OrthoDBiEOG664CMF.
PhylomeDBiQ9I7A8.

Family and domain databases

Gene3Di3.90.45.10. 1 hit.
HAMAPiMF_00163. Pep_deformylase.
InterProiIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
PANTHERiPTHR10458. PTHR10458. 1 hit.
PfamiPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004749. Pep_def. 1 hit.
PRINTSiPR01576. PDEFORMYLASE.
SUPFAMiSSF56420. SSF56420. 1 hit.
TIGRFAMsiTIGR00079. pept_deformyl. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9I7A8-1 [UniParc]FASTAAdd to Basket

« Hide

MAILNILEFP DPRLRTIAKP VEVVDDAVRQ LIDDMFETMY EAPGIGLAAT    50
QVNVHKRIVV MDLSEDKSEP RVFINPEFEP LTEDMDQYQE GCLSVPGFYE 100
NVDRPQKVRI KALDRDGNPF EEVAEGLLAV CIQHECDHLN GKLFVDYLST 150
LKRDRIRKKL EKQHRQQA 168
Length:168
Mass (Da):19,365
Last modified:March 1, 2001 - v1
Checksum:iA95F6B921E5F3189
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004091 Genomic DNA. Translation: AAG03409.1.
PIRiH83643.
RefSeqiNP_248709.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG03409; AAG03409; PA0019.
GeneIDi879306.
KEGGipae:PA0019.
PATRICi19834232. VBIPseAer58763_0018.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004091 Genomic DNA. Translation: AAG03409.1 .
PIRi H83643.
RefSeqi NP_248709.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1IX1 X-ray 1.85 A/B 1-168 [» ]
1LRY X-ray 2.60 A 2-168 [» ]
1N5N X-ray 1.80 A/B 1-168 [» ]
1S17 X-ray 1.95 A/B 1-168 [» ]
ProteinModelPortali Q9I7A8.
SMRi Q9I7A8. Positions 2-168.
ModBasei Search...

Protein-protein interaction databases

STRINGi 208964.PA0019.

Chemistry

BindingDBi Q9I7A8.
ChEMBLi CHEMBL1649054.

Protocols and materials databases

DNASUi 879306.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG03409 ; AAG03409 ; PA0019 .
GeneIDi 879306.
KEGGi pae:PA0019.
PATRICi 19834232. VBIPseAer58763_0018.

Organism-specific databases

PseudoCAPi PA0019.

Phylogenomic databases

eggNOGi COG0242.
KOi K01462.
OMAi DMYDTMD.
OrthoDBi EOG664CMF.
PhylomeDBi Q9I7A8.

Miscellaneous databases

EvolutionaryTracei Q9I7A8.
PROi Q9I7A8.

Family and domain databases

Gene3Di 3.90.45.10. 1 hit.
HAMAPi MF_00163. Pep_deformylase.
InterProi IPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view ]
PANTHERi PTHR10458. PTHR10458. 1 hit.
Pfami PF01327. Pep_deformylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF004749. Pep_def. 1 hit.
PRINTSi PR01576. PDEFORMYLASE.
SUPFAMi SSF56420. SSF56420. 1 hit.
TIGRFAMsi TIGR00079. pept_deformyl. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiDEF_PSEAE
AccessioniPrimary (citable) accession number: Q9I7A8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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