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Q9I7A8

- DEF_PSEAE

UniProt

Q9I7A8 - DEF_PSEAE

Protein

Peptide deformylase

Gene

def

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.UniRule annotation

    Catalytic activityi

    Formyl-L-methionyl peptide + H2O = formate + methionyl peptide.UniRule annotation

    Cofactori

    Binds 1 Fe2+ ion.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi92 – 921IronUniRule annotation
    Metal bindingi134 – 1341IronUniRule annotation
    Active sitei135 – 1351UniRule annotation
    Metal bindingi138 – 1381IronUniRule annotation

    GO - Molecular functioni

    1. iron ion binding Source: InterPro
    2. peptide deformylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. translation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peptide deformylaseUniRule annotation (EC:3.5.1.88UniRule annotation)
    Short name:
    PDFUniRule annotation
    Alternative name(s):
    Polypeptide deformylaseUniRule annotation
    Gene namesi
    Name:defUniRule annotation
    Ordered Locus Names:PA0019
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA0019.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 168168Peptide deformylasePRO_0000082819Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi208964.PA0019.

    Structurei

    Secondary structure

    1
    168
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi12 – 154
    Helixi26 – 4116
    Beta strandi45 – 484
    Helixi49 – 524
    Beta strandi56 – 627
    Beta strandi64 – 674
    Beta strandi70 – 8112
    Beta strandi85 – 906
    Beta strandi100 – 1067
    Beta strandi108 – 1136
    Beta strandi119 – 1246
    Helixi126 – 13914
    Helixi144 – 1474
    Helixi150 – 16617

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1IX1X-ray1.85A/B1-168[»]
    1LRYX-ray2.60A2-168[»]
    1N5NX-ray1.80A/B1-168[»]
    1S17X-ray1.95A/B1-168[»]
    ProteinModelPortaliQ9I7A8.
    SMRiQ9I7A8. Positions 2-168.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9I7A8.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the polypeptide deformylase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0242.
    KOiK01462.
    OMAiDMYDTMD.
    OrthoDBiEOG664CMF.
    PhylomeDBiQ9I7A8.

    Family and domain databases

    Gene3Di3.90.45.10. 1 hit.
    HAMAPiMF_00163. Pep_deformylase.
    InterProiIPR000181. Fmet_deformylase.
    IPR023635. Peptide_deformylase.
    [Graphical view]
    PANTHERiPTHR10458. PTHR10458. 1 hit.
    PfamiPF01327. Pep_deformylase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004749. Pep_def. 1 hit.
    PRINTSiPR01576. PDEFORMYLASE.
    SUPFAMiSSF56420. SSF56420. 1 hit.
    TIGRFAMsiTIGR00079. pept_deformyl. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9I7A8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAILNILEFP DPRLRTIAKP VEVVDDAVRQ LIDDMFETMY EAPGIGLAAT    50
    QVNVHKRIVV MDLSEDKSEP RVFINPEFEP LTEDMDQYQE GCLSVPGFYE 100
    NVDRPQKVRI KALDRDGNPF EEVAEGLLAV CIQHECDHLN GKLFVDYLST 150
    LKRDRIRKKL EKQHRQQA 168
    Length:168
    Mass (Da):19,365
    Last modified:March 1, 2001 - v1
    Checksum:iA95F6B921E5F3189
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG03409.1.
    PIRiH83643.
    RefSeqiNP_248709.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG03409; AAG03409; PA0019.
    GeneIDi879306.
    KEGGipae:PA0019.
    PATRICi19834232. VBIPseAer58763_0018.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG03409.1 .
    PIRi H83643.
    RefSeqi NP_248709.1. NC_002516.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1IX1 X-ray 1.85 A/B 1-168 [» ]
    1LRY X-ray 2.60 A 2-168 [» ]
    1N5N X-ray 1.80 A/B 1-168 [» ]
    1S17 X-ray 1.95 A/B 1-168 [» ]
    ProteinModelPortali Q9I7A8.
    SMRi Q9I7A8. Positions 2-168.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA0019.

    Chemistry

    BindingDBi Q9I7A8.
    ChEMBLi CHEMBL1649054.

    Protocols and materials databases

    DNASUi 879306.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG03409 ; AAG03409 ; PA0019 .
    GeneIDi 879306.
    KEGGi pae:PA0019.
    PATRICi 19834232. VBIPseAer58763_0018.

    Organism-specific databases

    PseudoCAPi PA0019.

    Phylogenomic databases

    eggNOGi COG0242.
    KOi K01462.
    OMAi DMYDTMD.
    OrthoDBi EOG664CMF.
    PhylomeDBi Q9I7A8.

    Miscellaneous databases

    EvolutionaryTracei Q9I7A8.
    PROi Q9I7A8.

    Family and domain databases

    Gene3Di 3.90.45.10. 1 hit.
    HAMAPi MF_00163. Pep_deformylase.
    InterProi IPR000181. Fmet_deformylase.
    IPR023635. Peptide_deformylase.
    [Graphical view ]
    PANTHERi PTHR10458. PTHR10458. 1 hit.
    Pfami PF01327. Pep_deformylase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004749. Pep_def. 1 hit.
    PRINTSi PR01576. PDEFORMYLASE.
    SUPFAMi SSF56420. SSF56420. 1 hit.
    TIGRFAMsi TIGR00079. pept_deformyl. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

    Entry informationi

    Entry nameiDEF_PSEAE
    AccessioniPrimary (citable) accession number: Q9I7A8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 5, 2001
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3