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Q9I7A8

- DEF_PSEAE

UniProt

Q9I7A8 - DEF_PSEAE

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Protein

Peptide deformylase

Gene

def

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.UniRule annotation

Catalytic activityi

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe(2+) ion.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi92 – 921IronUniRule annotation
Metal bindingi134 – 1341IronUniRule annotation
Active sitei135 – 1351UniRule annotation
Metal bindingi138 – 1381IronUniRule annotation

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. peptide deformylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide deformylaseUniRule annotation (EC:3.5.1.88UniRule annotation)
Short name:
PDFUniRule annotation
Alternative name(s):
Polypeptide deformylaseUniRule annotation
Gene namesi
Name:defUniRule annotation
Ordered Locus Names:PA0019
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA0019.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 168168Peptide deformylasePRO_0000082819Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi208964.PA0019.

Structurei

Secondary structure

1
168
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 154Combined sources
Helixi26 – 4116Combined sources
Beta strandi45 – 484Combined sources
Helixi49 – 524Combined sources
Beta strandi56 – 627Combined sources
Beta strandi64 – 674Combined sources
Beta strandi70 – 8112Combined sources
Beta strandi85 – 906Combined sources
Beta strandi100 – 1067Combined sources
Beta strandi108 – 1136Combined sources
Beta strandi119 – 1246Combined sources
Helixi126 – 13914Combined sources
Helixi144 – 1474Combined sources
Helixi150 – 16617Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IX1X-ray1.85A/B1-168[»]
1LRYX-ray2.60A2-168[»]
1N5NX-ray1.80A/B1-168[»]
1S17X-ray1.95A/B1-168[»]
ProteinModelPortaliQ9I7A8.
SMRiQ9I7A8. Positions 2-168.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9I7A8.

Family & Domainsi

Sequence similaritiesi

Belongs to the polypeptide deformylase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0242.
InParanoidiQ9I7A8.
KOiK01462.
OMAiDMYDTMD.
OrthoDBiEOG664CMF.
PhylomeDBiQ9I7A8.

Family and domain databases

Gene3Di3.90.45.10. 1 hit.
HAMAPiMF_00163. Pep_deformylase.
InterProiIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
PANTHERiPTHR10458. PTHR10458. 1 hit.
PfamiPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004749. Pep_def. 1 hit.
PRINTSiPR01576. PDEFORMYLASE.
SUPFAMiSSF56420. SSF56420. 1 hit.
TIGRFAMsiTIGR00079. pept_deformyl. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9I7A8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAILNILEFP DPRLRTIAKP VEVVDDAVRQ LIDDMFETMY EAPGIGLAAT
60 70 80 90 100
QVNVHKRIVV MDLSEDKSEP RVFINPEFEP LTEDMDQYQE GCLSVPGFYE
110 120 130 140 150
NVDRPQKVRI KALDRDGNPF EEVAEGLLAV CIQHECDHLN GKLFVDYLST
160
LKRDRIRKKL EKQHRQQA
Length:168
Mass (Da):19,365
Last modified:March 1, 2001 - v1
Checksum:iA95F6B921E5F3189
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03409.1.
PIRiH83643.
RefSeqiNP_248709.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG03409; AAG03409; PA0019.
GeneIDi879306.
KEGGipae:PA0019.
PATRICi19834232. VBIPseAer58763_0018.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03409.1 .
PIRi H83643.
RefSeqi NP_248709.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1IX1 X-ray 1.85 A/B 1-168 [» ]
1LRY X-ray 2.60 A 2-168 [» ]
1N5N X-ray 1.80 A/B 1-168 [» ]
1S17 X-ray 1.95 A/B 1-168 [» ]
ProteinModelPortali Q9I7A8.
SMRi Q9I7A8. Positions 2-168.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 208964.PA0019.

Chemistry

BindingDBi Q9I7A8.
ChEMBLi CHEMBL1649054.

Protocols and materials databases

DNASUi 879306.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG03409 ; AAG03409 ; PA0019 .
GeneIDi 879306.
KEGGi pae:PA0019.
PATRICi 19834232. VBIPseAer58763_0018.

Organism-specific databases

PseudoCAPi PA0019.

Phylogenomic databases

eggNOGi COG0242.
InParanoidi Q9I7A8.
KOi K01462.
OMAi DMYDTMD.
OrthoDBi EOG664CMF.
PhylomeDBi Q9I7A8.

Miscellaneous databases

EvolutionaryTracei Q9I7A8.
PROi Q9I7A8.

Family and domain databases

Gene3Di 3.90.45.10. 1 hit.
HAMAPi MF_00163. Pep_deformylase.
InterProi IPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view ]
PANTHERi PTHR10458. PTHR10458. 1 hit.
Pfami PF01327. Pep_deformylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF004749. Pep_def. 1 hit.
PRINTSi PR01576. PDEFORMYLASE.
SUPFAMi SSF56420. SSF56420. 1 hit.
TIGRFAMsi TIGR00079. pept_deformyl. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiDEF_PSEAE
AccessioniPrimary (citable) accession number: Q9I7A8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 5, 2001
Last sequence update: March 1, 2001
Last modified: November 26, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3