Q9I767 (5NTD_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5'-nucleotidase
EC=3.1.3.5

Alternative name(s):
Nucleoside 5'-monophosphate phosphohydrolase

Gene names

Ordered Locus Names:

PA0065

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Specifically dephosphorylates nucleoside 5'-monophosphates to nucleosides and inorganic phosphate. Displays high activity toward 5'-UMP and 5'-IMP, significant activity against 5'-XMP and 5'-TMP, and low activity against 5'-CMP. Ref.2
Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.
Cofactor	Divalent metal cation. Prefers manganese over magnesium. Ref.2
Sequence similarities	Belongs to the HAD-like hydrolase superfamily.
Biophysicochemical properties	Kinetic parameters: KM=0.39 mM for 5'-UMP Ref.2 Vmax=3.14 µmol/min/mg enzyme with 5'-UMP as substrate pH dependence: Optimum pH is 7.4.

Ontologies

Keywords
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	5'-nucleotidase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	5'-nucleotidase		PRO_0000064390
Sites
	Active site	14	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I767 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 8885AF689CC828B1	FASTA	221	24,951
	10 20 30 40 50 60 MRDAALRYPN ILFDLDGTLT DPREGITRSV QFALARLGID EPDLARLEHF IGPPLLQCFM 70 80 90 100 110 120 QTYGFDEARA WEAVNHYRER FRVTGLYENR VFDGIPELLE ALVGRGHTLY VATSKPGVFA 130 140 150 160 170 180 REIARHFAFD RHFKAIYGSE LDGTRTHKEE LIRHLLDSEG LAAEHCLMIG DRMHDLLGAS 190 200 210 220 RNGVACIGVG YGFGSEDELR AHQPTHYCAD LAALRQVLES H

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Enzyme genomics: application of general enzymatic screens to discover new enzymes." Kuznetsova E., Proudfoot M., Sanders S.A., Reinking J., Savchenko A., Arrowsmith C.H., Edwards A.M., Yakunin A.F. FEMS Microbiol. Rev. 29:263-279(2005) [PubMed: 15808744] [Abstract] Cited for: FUNCTION, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG03455.1.
PIR	C83636.
RefSeq	NP_248755.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9I767.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	878495.
GenomeReviews	Gene locus PA0065 in contig AE004091_GR.
KEGG	pae:PA0065.
NMPDR	fig|208964.1.peg.65.
PATRIC	19834332. VBIPseAer58763_0068.
Organism-specific databases
PseudoCAP	PA0065.
Phylogenomic databases
HOGENOM	HBG742904.
OMA	TLYIATS.
ProtClustDB	CLSK865566.
Enzyme and pathway databases
BioCyc	PAER208964:PA0065-MONOMER.
BRENDA	3.1.3.5. 5087.
Family and domain databases
InterPro	IPR005834. Dehalogen-like_hydro. IPR023214. HAD-like_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.1000. HAD-like_dom. 1 hit.
KO	K01091.
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
SUPFAM	SSF56784. HAD-like_dom. 1 hit.
ProtoNet	Search...

Entry information

Entry name

5NTD_PSEAE

Accession

Primary (citable) accession number: Q9I767

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program