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Protein

Beta-alanine--pyruvate aminotransferase

Gene

bauA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the degradation of beta-alanine. Catalyzes the transfer of the amino group from beta-alanine to pyruvate to yield L-alanine and 3-oxopropanoate. It can also accept both 4-aminobutyrate and (S)-alpha-methylbenzylamine (MBA) as amino-group donors in the presence of pyruvate as an amine acceptor.2 Publications

Catalytic activityi

L-alanine + 3-oxopropanoate = pyruvate + beta-alanine.

Cofactori

pyridoxal 5'-phosphate2 Publications

Enzyme regulationi

Inhibited by gabaculine (5-amino-1,3-cyclohexadienylcarboxylic acid).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61Substrate1 Publication1
Binding sitei327Pyridoxal phosphate2 Publications1
Binding sitei414Substrate1 Publication1
Binding sitei421Substrate1 Publication1

GO - Molecular functioni

GO - Biological processi

  • beta-alanine biosynthetic process Source: PseudoCAP
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate, Pyruvate

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-alanine--pyruvate aminotransferase (EC:2.6.1.18)
Short name:
Beta-A--Py AT
Alternative name(s):
Beta-alanine--pyruvate transaminase
Omega-amino acid aminotransferase
Short name:
Omega-amino acid AT
Omega-amino acid--pyruvate aminotransferase
Short name:
Omega-APT
Gene namesi
Name:bauA
Ordered Locus Names:PA0132
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0132.

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene grow normally on putrescine, cadaverine, and GABA, but growth on beta-alanine is completely abolished.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004289711 – 448Beta-alanine--pyruvate aminotransferaseAdd BLAST448

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei288N6-(pyridoxal phosphate)lysine1

Proteomic databases

PaxDbiQ9I700.

Expressioni

Inductioni

Activated by BauR.1 Publication

Interactioni

Subunit structurei

Homotetramer.2 Publications

GO - Molecular functioni

Protein-protein interaction databases

STRINGi208964.PA0132.

Structurei

Secondary structure

1448
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 14Combined sources3
Helixi17 – 19Combined sources3
Helixi27 – 32Combined sources6
Beta strandi36 – 42Combined sources7
Beta strandi44 – 47Combined sources4
Beta strandi52 – 55Combined sources4
Helixi58 – 61Combined sources4
Helixi70 – 82Combined sources13
Beta strandi88 – 90Combined sources3
Helixi94 – 105Combined sources12
Beta strandi111 – 119Combined sources9
Helixi120 – 137Combined sources18
Beta strandi145 – 149Combined sources5
Helixi158 – 163Combined sources6
Helixi167 – 170Combined sources4
Beta strandi180 – 182Combined sources3
Beta strandi198 – 200Combined sources3
Helixi201 – 205Combined sources5
Helixi207 – 215Combined sources9
Helixi217 – 219Combined sources3
Beta strandi220 – 225Combined sources6
Beta strandi227 – 229Combined sources3
Turni230 – 233Combined sources4
Helixi241 – 252Combined sources12
Beta strandi255 – 259Combined sources5
Turni261 – 268Combined sources8
Beta strandi269 – 271Combined sources3
Helixi273 – 277Combined sources5
Beta strandi282 – 286Combined sources5
Helixi288 – 291Combined sources4
Beta strandi298 – 303Combined sources6
Helixi304 – 311Combined sources8
Turni327 – 330Combined sources4
Helixi332 – 347Combined sources16
Helixi350 – 366Combined sources17
Turni367 – 370Combined sources4
Beta strandi374 – 380Combined sources7
Beta strandi383 – 388Combined sources6
Helixi397 – 408Combined sources12
Beta strandi414 – 416Combined sources3
Beta strandi419 – 422Combined sources4
Helixi430 – 445Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4B98X-ray1.65A/B/C/D1-448[»]
4B9BX-ray1.64A/B/C/D/E/F/G/H1-448[»]
4BQ0X-ray1.77A/B/C/D1-448[»]
ProteinModelPortaliQ9I700.
SMRiQ9I700.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni120 – 121Pyridoxal phosphate binding2

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108JPX. Bacteria.
COG0161. LUCA.
InParanoidiQ9I700.
KOiK00822.
OMAiKLDAHWM.
PhylomeDBiQ9I700.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
InterProiIPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9I700-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQPLNVAPP VSSELNLRAH WMPFSANRNF QKDPRIIVAA EGSWLTDDKG
60 70 80 90 100
RKVYDSLSGL WTCGAGHSRK EIQEAVARQL GTLDYSPGFQ YGHPLSFQLA
110 120 130 140 150
EKIAGLLPGE LNHVFFTGSG SECADTSIKM ARAYWRLKGQ PQKTKLIGRA
160 170 180 190 200
RGYHGVNVAG TSLGGIGGNR KMFGQLMDVD HLPHTLQPGM AFTRGMAQTG
210 220 230 240 250
GVELANELLK LIELHDASNI AAVIVEPMSG SAGVLVPPVG YLQRLREICD
260 270 280 290 300
QHNILLIFDE VITAFGRLGT YSGAEYFGVT PDLMNVAKQV TNGAVPMGAV
310 320 330 340 350
IASSEIYDTF MNQALPEHAV EFSHGYTYSA HPVACAAGLA ALDILARDNL
360 370 380 390 400
VQQSAELAPH FEKGLHGLQG AKNVIDIRNC GLAGAIQIAP RDGDPTVRPF
410 420 430 440
EAGMKLWQQG FYVRFGGDTL QFGPTFNARP EELDRLFDAV GEALNGIA
Length:448
Mass (Da):48,382
Last modified:March 1, 2001 - v1
Checksum:iBEC87B105289BD1E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03522.1.
PIRiF83628.
RefSeqiNP_248822.1. NC_002516.2.
WP_003083801.1. NZ_ASJY01000030.1.

Genome annotation databases

EnsemblBacteriaiAAG03522; AAG03522; PA0132.
GeneIDi879350.
KEGGipae:PA0132.
PATRICi19834470. VBIPseAer58763_0137.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03522.1.
PIRiF83628.
RefSeqiNP_248822.1. NC_002516.2.
WP_003083801.1. NZ_ASJY01000030.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4B98X-ray1.65A/B/C/D1-448[»]
4B9BX-ray1.64A/B/C/D/E/F/G/H1-448[»]
4BQ0X-ray1.77A/B/C/D1-448[»]
ProteinModelPortaliQ9I700.
SMRiQ9I700.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0132.

Proteomic databases

PaxDbiQ9I700.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG03522; AAG03522; PA0132.
GeneIDi879350.
KEGGipae:PA0132.
PATRICi19834470. VBIPseAer58763_0137.

Organism-specific databases

PseudoCAPiPA0132.

Phylogenomic databases

eggNOGiENOG4108JPX. Bacteria.
COG0161. LUCA.
InParanoidiQ9I700.
KOiK00822.
OMAiKLDAHWM.
PhylomeDBiQ9I700.

Family and domain databases

CDDicd00610. OAT_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 2 hits.
InterProiIPR005814. Aminotrans_3.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11986. PTHR11986. 1 hit.
PfamiPF00202. Aminotran_3. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00600. AA_TRANSFER_CLASS_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBAUA_PSEAE
AccessioniPrimary (citable) accession number: Q9I700
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 14, 2014
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.