Reviewed, UniProtKB/Swiss-Prot Q9I6Z2 (AHPF_PSEAE)

Names and origin

Protein names

Recommended name:
    Alkyl hydroperoxide reductase subunit F
    EC=1.6.4.-

Gene names

Name:	ahpF
Ordered Locus Names:	PA0140

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	521 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the ahpC protein By similarity.
Cofactor	Binds 1 FAD per subunit By similarity.
Subunit structure	Homodimer By similarity.
Miscellaneous	The active site is a redox-active disulfide bond By similarity.
Sequence similarities	Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family.

Ontologies

Keywords
Domain	Redox-active center
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro NAD or NADH binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on NADH or NADPH Inferred from electronic annotation. Source: InterPro protein disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 521	521	Alkyl hydroperoxide reductase subunit F		PRO_0000287807
Regions
	Nucleotide binding	213 – 228	16	FAD By similarity
	Nucleotide binding	356 – 370	15	NAD or NADP By similarity
	Nucleotide binding	477 – 487	11	FAD By similarity
Amino acid modifications
	Disulfide bond	344 ↔ 347		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I6Z2-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 58BD97E3AE1E32BA	FASTA	521	55,836
	10 20 30 40 50 60 MLDANLKTQL KAYLEKVSQP FEIVASLDDS DKSRELLGLL QDIVGLTDKI TLKTDGSDAR 70 80 90 100 110 120 KPSFSLNRPG ADIGLRFAGI PMGHEFTSLV LALLQVGGHP SKLDADVIEQ VKGIEGTFEF 130 140 150 160 170 180 ETYFSLSCQN CPDVVQALNL MAVLNPNIRH VAIDGALFQD EVEARQIMSV PSIYLNGEVF 190 200 210 220 230 240 GQGRMGVEEI LAKIDTGAAA RDAEKLTARD AFDVLVVGGG PAGAAAAIYA ARKGIRTGVA 250 260 270 280 290 300 AERFGGQVLD TMAIENFISV QETEGPKLAR ALEEHVRHYE VDIMNLQRAS KLVPAKNAGE 310 320 330 340 350 360 LHEVRFESGG SLKAKTLILA TGARWREMGV PGEQEYKAKG VCFCPHCDGP LFKGKRVAVI 370 380 390 400 410 420 GGGNSGVEAA IDLAGIVAHV TLLEFDSKLR ADAVLQRKLY SLPNVEVITS ALTSEVKGDG 430 440 450 460 470 480 QKVTGLVYKD RNSEEFKSIE LEGIFVQIGL LPNTEWLKGS VELSPRGEII VDARGETSLP 490 500 510 520 GIFAAGDVTT VPYKQIVIAV GEGAKASLSA FDHLIRTSAP E

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004091 Genomic DNA. Translation: AAG03530.1.
PIR	D83627.
RefSeq	NP_248830.1.
3D structure databases
HSSP	HSSP built from PDB template 1FL2 based on UniProtKB P35340.
ModBase	Search...
Genome annotation databases
GeneID	879326.
GenomeReviews	Gene locus PA0140 in contig AE004091_GR.
KEGG	pae:PA0140.
NMPDR	fig|208964.1.peg.140.
Organism-specific databases
PseudoCAP	PA0140.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9I6Z2.
OMA	Q9I6Z2. ELEGVFV.
Enzyme and pathway databases
BioCyc	PAER208964:PA0140-MON.
Family and domain databases
InterPro	IPR012081. Alkyl_hydroperoxide_Rdtase_suF. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR002109. Glutaredoxin. IPR008255. Pyr_nucl-diS_OxRdtase_2_AS. IPR001327. Pyr_OxRdtase_NAD_bd. IPR000103. Pyridine_nuc-diS_OxRdtase_2. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 2 hits.
Pfam	PF00070. Pyr_redox. 2 hits. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PIRSF	PIRSF000238. AhpF. 1 hit.
PRINTS	PR00368. FADPNR. PR00469. PNDRDTASEII.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03140. AhpF. 1 hit.
PROSITE	PS51354. GLUTAREDOXIN_2. 1 hit. PS00573. PYRIDINE_REDOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AHPF_PSEAE

Accession

Primary (citable) accession number: Q9I6Z2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)