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Protein

Adenine deaminase

Gene

PA0148

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolytic deamination of adenine to hypoxanthine. Plays an important role in the purine salvage pathway and in nitrogen catabolism.UniRule annotation

Catalytic activityi

Adenine + H2O = hypoxanthine + NH3.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi14Zinc; catalytic1
Metal bindingi16Zinc; catalytic1
Metal bindingi194Zinc; catalytic1
Active sitei197Proton donor1
Sitei218Important for catalytic activityUniRule annotation1
Metal bindingi275Zinc; catalytic1
Binding sitei276Substrate1

GO - Molecular functioni

  • adenine deaminase activity Source: PseudoCAP
  • zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine deaminaseUniRule annotation (EC:3.5.4.2UniRule annotation)
Short name:
ADEUniRule annotation
Alternative name(s):
Adenine aminohydrolaseUniRule annotation
Short name:
AAHUniRule annotation
Gene namesi
Ordered Locus Names:PA0148
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0148.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001943771 – 316Adenine deaminaseAdd BLAST316

Proteomic databases

PaxDbiQ9I6Y4.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA0148.

Structurei

Secondary structure

1316
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 7Combined sources5
Beta strandi10 – 12Combined sources3
Beta strandi14 – 16Combined sources3
Helixi17 – 20Combined sources4
Helixi23 – 32Combined sources10
Beta strandi38 – 41Combined sources4
Helixi42 – 47Combined sources6
Helixi54 – 64Combined sources11
Helixi65 – 67Combined sources3
Helixi71 – 87Combined sources17
Beta strandi90 – 93Combined sources4
Helixi99 – 103Combined sources5
Turni104 – 106Combined sources3
Helixi109 – 127Combined sources19
Beta strandi134 – 138Combined sources5
Helixi143 – 153Combined sources11
Helixi154 – 159Combined sources6
Beta strandi161 – 167Combined sources7
Helixi174 – 177Combined sources4
Helixi178 – 186Combined sources9
Beta strandi193 – 199Combined sources7
Helixi201 – 209Combined sources9
Beta strandi214 – 218Combined sources5
Helixi220 – 224Combined sources5
Helixi226 – 235Combined sources10
Beta strandi239 – 241Combined sources3
Helixi243 – 248Combined sources6
Beta strandi251 – 254Combined sources4
Helixi255 – 257Combined sources3
Helixi260 – 266Combined sources7
Beta strandi270 – 272Combined sources3
Helixi277 – 280Combined sources4
Helixi284 – 295Combined sources12
Helixi299 – 311Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OU8X-ray2.51A/B2-316[»]
3PANX-ray2.63A/B2-316[»]
3PAOX-ray2.49A/B2-316[»]
3PBMX-ray2.59A/B2-316[»]
ProteinModelPortaliQ9I6Y4.
SMRiQ9I6Y4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9I6Y4.

Family & Domainsi

Sequence similaritiesi

Belongs to the adenosine and AMP deaminases family. Adenine deaminase type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EKD. Bacteria.
COG1816. LUCA.
HOGENOMiHOG000218813.
InParanoidiQ9I6Y4.
KOiK01488.
OMAiDHGVRAI.
PhylomeDBiQ9I6Y4.

Family and domain databases

CDDicd01320. ADA. 1 hit.
HAMAPiMF_01962. Adenine_deaminase. 1 hit.
InterProiIPR001365. A/AMP_deaminase_dom.
IPR028892. ADE.
IPR006330. Ado/ade_deaminase.
IPR032466. Metal_Hydrolase.
[Graphical view]
PfamiPF00962. A_deaminase. 1 hit.
[Graphical view]
SUPFAMiSSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR01430. aden_deam. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9I6Y4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYEWLNALPK AELHLHLEGT LEPELLFALA ERNRIALPWN DVETLRKAYA
60 70 80 90 100
FNNLQEFLDL YYAGADVLRT EQDFYDLTWA YLQKCKAQNV VHVEPFFDPQ
110 120 130 140 150
THTDRGIPFE VVLAGIRAAL RDGEKLLGIR HGLILSFLRH LSEEQAQKTL
160 170 180 190 200
DQALPFRDAF IAVGLDSSEV GHPPSKFQRV FDRARSEGFL TVAHAGEEGP
210 220 230 240 250
PEYIWEALDL LKVERIDHGV RAFEDERLMR RLIDEQIPLT VCPLSNTKLC
260 270 280 290 300
VFDDMSQHTI LDMLERGVKV TVNSDDPAYF GGYVTENFHA LQQSLGMTEE
310
QARRLAQNSL DARLVK
Length:316
Mass (Da):36,163
Last modified:March 1, 2001 - v1
Checksum:iEFB41B00CBFDED4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03538.1.
PIRiH83625.
RefSeqiNP_248838.1. NC_002516.2.
WP_003112641.1. NZ_ASJY01000034.1.

Genome annotation databases

EnsemblBacteriaiAAG03538; AAG03538; PA0148.
GeneIDi879249.
KEGGipae:PA0148.
PATRICi19834504. VBIPseAer58763_0154.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03538.1.
PIRiH83625.
RefSeqiNP_248838.1. NC_002516.2.
WP_003112641.1. NZ_ASJY01000034.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3OU8X-ray2.51A/B2-316[»]
3PANX-ray2.63A/B2-316[»]
3PAOX-ray2.49A/B2-316[»]
3PBMX-ray2.59A/B2-316[»]
ProteinModelPortaliQ9I6Y4.
SMRiQ9I6Y4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0148.

Proteomic databases

PaxDbiQ9I6Y4.

Protocols and materials databases

DNASUi879249.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG03538; AAG03538; PA0148.
GeneIDi879249.
KEGGipae:PA0148.
PATRICi19834504. VBIPseAer58763_0154.

Organism-specific databases

PseudoCAPiPA0148.

Phylogenomic databases

eggNOGiENOG4105EKD. Bacteria.
COG1816. LUCA.
HOGENOMiHOG000218813.
InParanoidiQ9I6Y4.
KOiK01488.
OMAiDHGVRAI.
PhylomeDBiQ9I6Y4.

Miscellaneous databases

EvolutionaryTraceiQ9I6Y4.

Family and domain databases

CDDicd01320. ADA. 1 hit.
HAMAPiMF_01962. Adenine_deaminase. 1 hit.
InterProiIPR001365. A/AMP_deaminase_dom.
IPR028892. ADE.
IPR006330. Ado/ade_deaminase.
IPR032466. Metal_Hydrolase.
[Graphical view]
PfamiPF00962. A_deaminase. 1 hit.
[Graphical view]
SUPFAMiSSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR01430. aden_deam. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiADE_PSEAE
AccessioniPrimary (citable) accession number: Q9I6Y4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.