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Protein

Adenine deaminase

Gene

PA0148

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolytic deamination of adenine to hypoxanthine. Plays an important role in the purine salvage pathway and in nitrogen catabolism.UniRule annotation

Catalytic activityi

Adenine + H2O = hypoxanthine + NH3.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi14 – 141Zinc; catalytic
Metal bindingi16 – 161Zinc; catalytic
Metal bindingi194 – 1941Zinc; catalytic
Active sitei197 – 1971Proton donor
Sitei218 – 2181Important for catalytic activityUniRule annotation
Metal bindingi275 – 2751Zinc; catalytic
Binding sitei276 – 2761Substrate

GO - Molecular functioni

  • adenine deaminase activity Source: PseudoCAP
  • zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Adenine deaminaseUniRule annotation (EC:3.5.4.2UniRule annotation)
Short name:
ADEUniRule annotation
Alternative name(s):
Adenine aminohydrolaseUniRule annotation
Short name:
AAHUniRule annotation
Gene namesi
Ordered Locus Names:PA0148
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0148.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 316316Adenine deaminasePRO_0000194377Add
BLAST

Proteomic databases

PaxDbiQ9I6Y4.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA0148.

Structurei

Secondary structure

1
316
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 75Combined sources
Beta strandi10 – 123Combined sources
Beta strandi14 – 163Combined sources
Helixi17 – 204Combined sources
Helixi23 – 3210Combined sources
Beta strandi38 – 414Combined sources
Helixi42 – 476Combined sources
Helixi54 – 6411Combined sources
Helixi65 – 673Combined sources
Helixi71 – 8717Combined sources
Beta strandi90 – 934Combined sources
Helixi99 – 1035Combined sources
Turni104 – 1063Combined sources
Helixi109 – 12719Combined sources
Beta strandi134 – 1385Combined sources
Helixi143 – 15311Combined sources
Helixi154 – 1596Combined sources
Beta strandi161 – 1677Combined sources
Helixi174 – 1774Combined sources
Helixi178 – 1869Combined sources
Beta strandi193 – 1997Combined sources
Helixi201 – 2099Combined sources
Beta strandi214 – 2185Combined sources
Helixi220 – 2245Combined sources
Helixi226 – 23510Combined sources
Beta strandi239 – 2413Combined sources
Helixi243 – 2486Combined sources
Beta strandi251 – 2544Combined sources
Helixi255 – 2573Combined sources
Helixi260 – 2667Combined sources
Beta strandi270 – 2723Combined sources
Helixi277 – 2804Combined sources
Helixi284 – 29512Combined sources
Helixi299 – 31113Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3OU8X-ray2.51A/B2-316[»]
3PANX-ray2.63A/B2-316[»]
3PAOX-ray2.49A/B2-316[»]
3PBMX-ray2.59A/B2-316[»]
ProteinModelPortaliQ9I6Y4.
SMRiQ9I6Y4. Positions 2-315.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9I6Y4.

Family & Domainsi

Sequence similaritiesi

Belongs to the adenosine and AMP deaminases family. Adenine deaminase type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EKD. Bacteria.
COG1816. LUCA.
HOGENOMiHOG000218813.
InParanoidiQ9I6Y4.
KOiK01488.
OMAiDHGVRAI.
PhylomeDBiQ9I6Y4.

Family and domain databases

HAMAPiMF_01962. Adenine_deaminase. 1 hit.
InterProiIPR001365. A/AMP_deaminase_dom.
IPR028892. ADE.
IPR006330. Ado/ade_deaminase.
IPR032466. Metal_Hydrolase.
[Graphical view]
PfamiPF00962. A_deaminase. 1 hit.
[Graphical view]
SUPFAMiSSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR01430. aden_deam. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9I6Y4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYEWLNALPK AELHLHLEGT LEPELLFALA ERNRIALPWN DVETLRKAYA
60 70 80 90 100
FNNLQEFLDL YYAGADVLRT EQDFYDLTWA YLQKCKAQNV VHVEPFFDPQ
110 120 130 140 150
THTDRGIPFE VVLAGIRAAL RDGEKLLGIR HGLILSFLRH LSEEQAQKTL
160 170 180 190 200
DQALPFRDAF IAVGLDSSEV GHPPSKFQRV FDRARSEGFL TVAHAGEEGP
210 220 230 240 250
PEYIWEALDL LKVERIDHGV RAFEDERLMR RLIDEQIPLT VCPLSNTKLC
260 270 280 290 300
VFDDMSQHTI LDMLERGVKV TVNSDDPAYF GGYVTENFHA LQQSLGMTEE
310
QARRLAQNSL DARLVK
Length:316
Mass (Da):36,163
Last modified:March 1, 2001 - v1
Checksum:iEFB41B00CBFDED4A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03538.1.
PIRiH83625.
RefSeqiNP_248838.1. NC_002516.2.
WP_003112641.1. NZ_ASJY01000034.1.

Genome annotation databases

EnsemblBacteriaiAAG03538; AAG03538; PA0148.
GeneIDi879249.
KEGGipae:PA0148.
PATRICi19834504. VBIPseAer58763_0154.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03538.1.
PIRiH83625.
RefSeqiNP_248838.1. NC_002516.2.
WP_003112641.1. NZ_ASJY01000034.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3OU8X-ray2.51A/B2-316[»]
3PANX-ray2.63A/B2-316[»]
3PAOX-ray2.49A/B2-316[»]
3PBMX-ray2.59A/B2-316[»]
ProteinModelPortaliQ9I6Y4.
SMRiQ9I6Y4. Positions 2-315.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0148.

Proteomic databases

PaxDbiQ9I6Y4.

Protocols and materials databases

DNASUi879249.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG03538; AAG03538; PA0148.
GeneIDi879249.
KEGGipae:PA0148.
PATRICi19834504. VBIPseAer58763_0154.

Organism-specific databases

PseudoCAPiPA0148.

Phylogenomic databases

eggNOGiENOG4105EKD. Bacteria.
COG1816. LUCA.
HOGENOMiHOG000218813.
InParanoidiQ9I6Y4.
KOiK01488.
OMAiDHGVRAI.
PhylomeDBiQ9I6Y4.

Miscellaneous databases

EvolutionaryTraceiQ9I6Y4.

Family and domain databases

HAMAPiMF_01962. Adenine_deaminase. 1 hit.
InterProiIPR001365. A/AMP_deaminase_dom.
IPR028892. ADE.
IPR006330. Ado/ade_deaminase.
IPR032466. Metal_Hydrolase.
[Graphical view]
PfamiPF00962. A_deaminase. 1 hit.
[Graphical view]
SUPFAMiSSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR01430. aden_deam. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiADE_PSEAE
AccessioniPrimary (citable) accession number: Q9I6Y4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.