Q9I6R0 (PCAF_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-ketoadipyl-CoA thiolase
EC=2.3.1.174

Alternative name(s):
3-oxoadipyl-CoA thiolase

Gene names

Name:	pcaF
Ordered Locus Names:	PA0228

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA By similarity.
Catalytic activity	Succinyl-CoA + acetyl-CoA = CoA + 3-oxoadipyl-CoA.
Pathway	Aromatic compound metabolism; beta-ketoadipate pathway; acetyl-CoA and succinyl-CoA from 3-oxoadipate: step 2/2.
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW protocatechuate catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	3-oxoadipyl-CoA thiolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 401	401	Beta-ketoadipyl-CoA thiolase		PRO_0000287819
Sites
	Active site	91	1	Acyl-thioester intermediate By similarity
	Active site	357	1	Proton acceptor By similarity
	Active site	387	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I6R0 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: BF8DF2798448D4DC	FASTA	401	42,106
	10 20 30 40 50 60 MSREVFICDA VRTPIGRFGG SLSAVRADDL AAVPLKALVE RNPGVDWSAL DEVFLGCANQ 70 80 90 100 110 120 AGEDNRNVAR MALLLAGLPE SVPGVTLNRL CASGMDAIGT AFRAIACGEM ELAIAGGVES 130 140 150 160 170 180 MSRAPYVMGK ADSAFGRGQK IEDTTIGWRF VNPLMKEQYG IDPMPQTADN VADDYRVSRA 190 200 210 220 230 240 DQDAFALRSQ QRAGRAQEAG FFAEEIVPVT IRGRKGDTLV EHDEHPRPDT TLEALARLKP 250 260 270 280 290 300 VNGPEKTVTA GNASGVNDGA AALVLASAEA VEKHGLTPRA RVLGMASAGV APRIMGIGPV 310 320 330 340 350 360 PAVRKLLRRL DLAIDAFDVI ELNEAFASQG LACLRELGVA DDSEKVNPNG GAIALGHPLG 370 380 390 400 MSGARLVLTA LHQLEKSGGR RGLATMCVGV GQGLALAIER V

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG03617.1.
PIR	D83618.
RefSeq	NP_248919.1. NC_002516.2.
3D structure databases
HSSP	HSSP built from PDB template 1AFW based on UniProtKB P27796.
ProteinModelPortal	Q9I6R0.
SMR	Q9I6R0. Positions 4-401.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	877715.
GenomeReviews	Gene locus PA0228 in contig AE004091_GR.
KEGG	pae:PA0228.
NMPDR	fig|208964.1.peg.229.
PATRIC	19834672. VBIPseAer58763_0238.
Organism-specific databases
PseudoCAP	PA0228.
Phylogenomic databases
HOGENOM	HBG370930.
OMA	MRDVFIC.
ProtClustDB	PRK09050.
Enzyme and pathway databases
BioCyc	PAER208964:PA0228-MONOMER.
Family and domain databases
InterPro	IPR012793. PcaF. IPR002155. Thiolase. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. IPR020615. Thiolase_acyl_enz_int_AS. IPR020610. Thiolase_AS. IPR020617. Thiolase_C. IPR020613. Thiolase_CS. IPR020616. Thiolase_N. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 4 hits.
KO	K07823.
PANTHER	PTHR18919. Thiolase. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit. TIGR02430. PcaF. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCAF_PSEAE

Accession

Primary (citable) accession number: Q9I6R0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program