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Protein

3-carboxy-cis,cis-muconate cycloisomerase

Gene

pcaB

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes an anti cycloisomerization.By similarity

Catalytic activityi

2-carboxy-2,5-dihydro-5-oxofuran-2-acetate = cis,cis-butadiene-1,2,4-tricarboxylate.

Pathwayi: beta-ketoadipate pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-oxo-4,5-dihydro-2-furylacetate from 3-carboxy-cis,cis-muconate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-carboxy-cis,cis-muconate cycloisomerase (pcaB)
  2. no protein annotated in this organism
This subpathway is part of the pathway beta-ketoadipate pathway, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-oxo-4,5-dihydro-2-furylacetate from 3-carboxy-cis,cis-muconate, the pathway beta-ketoadipate pathway and in Aromatic compound metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Enzyme and pathway databases

UniPathwayiUPA00157; UER00265.

Names & Taxonomyi

Protein namesi
Recommended name:
3-carboxy-cis,cis-muconate cycloisomerase (EC:5.5.1.2)
Alternative name(s):
3-carboxymuconate lactonizing enzyme
Short name:
CMLE
Gene namesi
Name:pcaB
Ordered Locus Names:PA0230
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0230.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4594593-carboxy-cis,cis-muconate cycloisomerasePRO_0000287772Add
BLAST

Proteomic databases

PaxDbiQ9I6Q8.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi208964.PA0230.

Structurei

3D structure databases

ProteinModelPortaliQ9I6Q8.
SMRiQ9I6Q8. Positions 7-450.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family.Curated

Phylogenomic databases

eggNOGiENOG4107RAW. Bacteria.
COG0015. LUCA.
InParanoidiQ9I6Q8.
KOiK01857.
OMAiHRATPMI.
OrthoDBiEOG686NDB.
PhylomeDBiQ9I6Q8.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
InterProiIPR019468. AdenyloSucc_lyase_C.
IPR024083. Fumarase/histidase_N.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
IPR012789. Protocat_PcaB.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiPF10397. ADSL_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SMARTiSM00998. ADSL_C. 1 hit.
[Graphical view]
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR02426. protocat_pcaB. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9I6Q8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDVPANQLF DAYFMAAPMR AVFSDRGRLQ GMLDFEAALA RAEARTGVVP
60 70 80 90 100
ATAVAPIEAA CRAELYDPLA LAEAVATAGN SAIPLVKALG RQVAAGDAEA
110 120 130 140 150
ERYVHLGATS QDAMDSGLVL QLRRALALLE QDLQRLAEVL ADQAERHADT
160 170 180 190 200
PLAGRTWLQH ATPVTLGMKL AGLLGALTRH RQRLRELRPR LLVLQFGGAS
210 220 230 240 250
GTLAALGEQA LPVAAALAEE LGLALPEQPW HTQRDRLVEF ASVLGLVAGS
260 270 280 290 300
LGKFGRDVSL LMQTEAGEVF EPAGAGRGGS STMPHKRNPV SSAVLIAAAT
310 320 330 340 350
RAPGLVSTLF AAMPQEHERS LGLWHAEWET LPELCCLVAG ALQQAIGLLE
360 370 380 390 400
GLEVDAQRMR RNLGLTHGLV LAEAVSIALA RRIGREAAHH LVEQCCRRAV
410 420 430 440 450
EQRRELRAVL GEEARVSAEL SGDELDRLLD PAHYLGQARA WVERALAEHH

ALGFEPHPA
Length:459
Mass (Da):49,121
Last modified:March 1, 2001 - v1
Checksum:iC43F6B3651F2D1D0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03619.1.
PIRiB83616.
RefSeqiNP_248921.1. NC_002516.2.
WP_003112675.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG03619; AAG03619; PA0230.
GeneIDi881957.
KEGGipae:PA0230.
PATRICi19834676. VBIPseAer58763_0240.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03619.1.
PIRiB83616.
RefSeqiNP_248921.1. NC_002516.2.
WP_003112675.1. NC_002516.2.

3D structure databases

ProteinModelPortaliQ9I6Q8.
SMRiQ9I6Q8. Positions 7-450.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0230.

Proteomic databases

PaxDbiQ9I6Q8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG03619; AAG03619; PA0230.
GeneIDi881957.
KEGGipae:PA0230.
PATRICi19834676. VBIPseAer58763_0240.

Organism-specific databases

PseudoCAPiPA0230.

Phylogenomic databases

eggNOGiENOG4107RAW. Bacteria.
COG0015. LUCA.
InParanoidiQ9I6Q8.
KOiK01857.
OMAiHRATPMI.
OrthoDBiEOG686NDB.
PhylomeDBiQ9I6Q8.

Enzyme and pathway databases

UniPathwayiUPA00157; UER00265.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
InterProiIPR019468. AdenyloSucc_lyase_C.
IPR024083. Fumarase/histidase_N.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
IPR012789. Protocat_PcaB.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiPF10397. ADSL_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SMARTiSM00998. ADSL_C. 1 hit.
[Graphical view]
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR02426. protocat_pcaB. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiPCAB_PSEAE
AccessioniPrimary (citable) accession number: Q9I6Q8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: March 1, 2001
Last modified: February 17, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.