Q9I6P3 (AROQ2_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-dehydroquinate dehydratase 2
Short name=3-dehydroquinase 2
EC=4.2.1.10

Alternative name(s):
Type II DHQase 2

Gene names

Name:	aroQ2
Ordered Locus Names:	PA0245

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a trans-dehydration via an enolate intermediate By similarity. HAMAP MF_00169
Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00169
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP MF_00169
Subunit structure	Homododecamer By similarity. HAMAP MF_00169
Sequence similarities	Belongs to the type-II 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 148	148	3-dehydroquinate dehydratase 2 HAMAP MF_00169		PRO_0000159919
Regions
	Region	102 – 103	2	Substrate binding By similarity
Sites
	Active site	24	1	Proton acceptor By similarity
	Active site	101	1	Proton donor By similarity
	Binding site	75	1	Substrate By similarity
	Binding site	81	1	Substrate By similarity
	Binding site	88	1	Substrate By similarity
	Binding site	112	1	Substrate By similarity
	Site	19	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I6P3 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 1F343909FD1655E3	FASTA	148	16,273
	10 20 30 40 50 60 MTRTVLVLNG PNLNLLGTRE PQTYGRRTLG EIADECAAFA ETRGFAVDFR QTNQEGQLLD 70 80 90 100 110 120 WIHQARGRVA GIVINPAAWT HTSVALRDAL AAVELPVVEV HLSNVHQREA FRHHSYVSPV 130 140 ALGVICGFGS LGYRLALEHF AERFEAAA

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG03634.1.
PIR	G83615.
RefSeq	NP_248936.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9I6P3.
SMR	Q9I6P3. Positions 3-143.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	882159.
GenomeReviews	Gene locus PA0245 in contig AE004091_GR.
KEGG	pae:PA0245.
PATRIC	19834706. VBIPseAer58763_0255.
Organism-specific databases
PseudoCAP	PA0245.
Phylogenomic databases
HOGENOM	HBG284657.
OMA	EFRQTNH.
ProtClustDB	PRK13015.
Enzyme and pathway databases
BioCyc	PAER208964:PA0245-MONOMER.
Family and domain databases
HAMAP	MF_00169. AroQ. [Tree]
InterPro	IPR001874. DHquinase_II. IPR018509. DHquinase_II_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.9100. DHquinase_II. 1 hit.
KO	K03786.
PANTHER	PTHR21272. DHquinase_II. 1 hit.
Pfam	PF01220. DHquinase_II. 1 hit. [Graphical view]
PIRSF	PIRSF001399. DHquinase_II. 1 hit.
ProDom	PD004527. DHquinase_II. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF52304. DHquinase_II. 1 hit.
TIGRFAMs	TIGR01088. AroQ. 1 hit.
PROSITE	PS01029. DEHYDROQUINASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROQ2_PSEAE

Accession

Primary (citable) accession number: Q9I6P3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program