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Protein

Spermidine-binding periplasmic protein SpuE

Gene

spuE

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Spermidine-binding protein probably required for its uptake into cells. Binds spermidine with high affinity (KD=14.3 nM). Does not bind putrescine, cadaverine or spermine. Spermidine binding induces large inter-domain conformational changes. Implicated in induction of type 3 secretion systems (T3SS), which play a role in virulence.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35Spermidine1 Publication1
Binding sitei181Spermidine1 Publication1
Binding sitei242Spermidine1 Publication1
Binding sitei269Spermidine1 Publication1

GO - Molecular functioni

  • spermidine binding Source: PseudoCAP

GO - Biological processi

  • pathogenesis Source: UniProtKB-KW
  • polyamine transport Source: PseudoCAP
Complete GO annotation...

Keywords - Biological processi

Transport, Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Spermidine-binding periplasmic protein SpuE
Gene namesi
Name:spuE
Ordered Locus Names:PA0301
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0301.

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Significant decrease in T3SS induction.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi271W → F: Increases affinity for putrescine (to KD=1.12 uM). 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000043139725 – 365Spermidine-binding periplasmic protein SpuESequence analysisAdd BLAST341

Proteomic databases

PaxDbiQ9I6J0.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA0301.

Structurei

Secondary structure

1365
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi29 – 34Combined sources6
Helixi42 – 50Combined sources9
Beta strandi54 – 61Combined sources8
Helixi62 – 70Combined sources9
Beta strandi77 – 79Combined sources3
Helixi83 – 91Combined sources9
Helixi100 – 102Combined sources3
Helixi104 – 108Combined sources5
Helixi111 – 117Combined sources7
Turni118 – 120Combined sources3
Helixi122 – 124Combined sources3
Beta strandi126 – 140Combined sources15
Helixi141 – 148Combined sources8
Helixi157 – 160Combined sources4
Helixi162 – 168Combined sources7
Helixi169 – 171Combined sources3
Beta strandi173 – 175Combined sources3
Helixi179 – 189Combined sources11
Helixi199 – 212Combined sources14
Helixi213 – 215Combined sources3
Beta strandi217 – 219Combined sources3
Helixi224 – 229Combined sources6
Beta strandi234 – 239Combined sources6
Helixi240 – 253Combined sources14
Beta strandi259 – 262Combined sources4
Beta strandi269 – 276Combined sources8
Helixi284 – 294Combined sources11
Helixi297 – 307Combined sources11
Beta strandi310 – 312Combined sources3
Helixi314 – 319Combined sources6
Helixi322 – 325Combined sources4
Turni328 – 330Combined sources3
Helixi334 – 337Combined sources4
Helixi348 – 361Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TTLX-ray2.30A/B28-362[»]
3TTNX-ray2.00A/B28-362[»]
ProteinModelPortaliQ9I6J0.
SMRiQ9I6J0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the bacterial solute-binding protein PotD/PotF family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108HM4. Bacteria.
COG0687. LUCA.
HOGENOMiHOG000263815.
KOiK11073.
OMAiSVYPPQA.
PhylomeDBiQ9I6J0.

Family and domain databases

InterProiIPR001188. Sperm_putr-bd.
[Graphical view]
PIRSFiPIRSF019574. Periplasmic_polyamine_BP. 1 hit.
PRINTSiPR00909. SPERMDNBNDNG.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9I6J0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQHSIGKTLL VAALATAIAG PVQAEKKSLH IYNWTDYIAP TTLKDFTKES
60 70 80 90 100
GIDVSYDVFD SNETLEGKLV SGHSGYDIVV PSNNFLGKQI QAGAFQKLDK
110 120 130 140 150
SKLPNWKNLD PALLKQLEVS DPGNQYAVPY LWGTNGIGYN VAKVKEVLGD
160 170 180 190 200
QPIDSWAILF EPENMKKLAK CGVAFMDSGD EMLPAALNYL GLDPNTHDPK
210 220 230 240 250
DYKKAEEVLT KVRPYVSYFH SSKYISDLAN GNICVAFGYS GDVFQAAARA
260 270 280 290 300
EEAGKGIDIQ YVIPKEGANL WFDLMAIPAD AKAADNAYAF IDYLLRPEVI
310 320 330 340 350
AKVSDYVGYA NAIPGARPLM DKSVSDSEEV YPPQAVLDKL YVSAVLPAKV
360
LRLQTRTWTR IKTGK
Length:365
Mass (Da):40,070
Last modified:March 1, 2001 - v1
Checksum:i3F14A63C3C1D417D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03690.1.
PIRiF83607.
RefSeqiNP_248992.1. NC_002516.2.
WP_003084301.1. NZ_ASJY01000065.1.

Genome annotation databases

EnsemblBacteriaiAAG03690; AAG03690; PA0301.
GeneIDi877626.
KEGGipae:PA0301.
PATRICi19834828. VBIPseAer58763_0315.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03690.1.
PIRiF83607.
RefSeqiNP_248992.1. NC_002516.2.
WP_003084301.1. NZ_ASJY01000065.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TTLX-ray2.30A/B28-362[»]
3TTNX-ray2.00A/B28-362[»]
ProteinModelPortaliQ9I6J0.
SMRiQ9I6J0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0301.

Proteomic databases

PaxDbiQ9I6J0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG03690; AAG03690; PA0301.
GeneIDi877626.
KEGGipae:PA0301.
PATRICi19834828. VBIPseAer58763_0315.

Organism-specific databases

PseudoCAPiPA0301.

Phylogenomic databases

eggNOGiENOG4108HM4. Bacteria.
COG0687. LUCA.
HOGENOMiHOG000263815.
KOiK11073.
OMAiSVYPPQA.
PhylomeDBiQ9I6J0.

Family and domain databases

InterProiIPR001188. Sperm_putr-bd.
[Graphical view]
PIRSFiPIRSF019574. Periplasmic_polyamine_BP. 1 hit.
PRINTSiPR00909. SPERMDNBNDNG.
ProtoNetiSearch...

Entry informationi

Entry nameiSPUE_PSEAE
AccessioniPrimary (citable) accession number: Q9I6J0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 7, 2015
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.