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Protein

Spermidine-binding periplasmic protein SpuE

Gene

spuE

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Spermidine-binding protein probably required for its uptake into cells. Binds spermidine with high affinity (KD=14.3 nM). Does not bind putrescine, cadaverine or spermine. Spermidine binding induces large inter-domain conformational changes. Implicated in induction of type 3 secretion systems (T3SS), which play a role in virulence.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351Spermidine1 Publication
Binding sitei181 – 1811Spermidine1 Publication
Binding sitei242 – 2421Spermidine1 Publication
Binding sitei269 – 2691Spermidine1 Publication

GO - Molecular functioni

  • spermidine binding Source: PseudoCAP

GO - Biological processi

  • pathogenesis Source: UniProtKB-KW
  • polyamine transport Source: PseudoCAP
Complete GO annotation...

Keywords - Biological processi

Transport, Virulence

Names & Taxonomyi

Protein namesi
Recommended name:
Spermidine-binding periplasmic protein SpuE
Gene namesi
Name:spuE
Ordered Locus Names:PA0301
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0301.

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Significant decrease in T3SS induction.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi271 – 2711W → F: Increases affinity for putrescine (to KD=1.12 uM). 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 365341Spermidine-binding periplasmic protein SpuESequence analysisPRO_0000431397Add
BLAST

Proteomic databases

PaxDbiQ9I6J0.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA0301.

Structurei

Secondary structure

1
365
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi29 – 346Combined sources
Helixi42 – 509Combined sources
Beta strandi54 – 618Combined sources
Helixi62 – 709Combined sources
Beta strandi77 – 793Combined sources
Helixi83 – 919Combined sources
Helixi100 – 1023Combined sources
Helixi104 – 1085Combined sources
Helixi111 – 1177Combined sources
Turni118 – 1203Combined sources
Helixi122 – 1243Combined sources
Beta strandi126 – 14015Combined sources
Helixi141 – 1488Combined sources
Helixi157 – 1604Combined sources
Helixi162 – 1687Combined sources
Helixi169 – 1713Combined sources
Beta strandi173 – 1753Combined sources
Helixi179 – 18911Combined sources
Helixi199 – 21214Combined sources
Helixi213 – 2153Combined sources
Beta strandi217 – 2193Combined sources
Helixi224 – 2296Combined sources
Beta strandi234 – 2396Combined sources
Helixi240 – 25314Combined sources
Beta strandi259 – 2624Combined sources
Beta strandi269 – 2768Combined sources
Helixi284 – 29411Combined sources
Helixi297 – 30711Combined sources
Beta strandi310 – 3123Combined sources
Helixi314 – 3196Combined sources
Helixi322 – 3254Combined sources
Turni328 – 3303Combined sources
Helixi334 – 3374Combined sources
Helixi348 – 36114Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TTLX-ray2.30A/B28-362[»]
3TTNX-ray2.00A/B28-362[»]
ProteinModelPortaliQ9I6J0.
SMRiQ9I6J0. Positions 28-362.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the bacterial solute-binding protein PotD/PotF family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108HM4. Bacteria.
COG0687. LUCA.
HOGENOMiHOG000263815.
KOiK11073.
OMAiSVYPPQA.
PhylomeDBiQ9I6J0.

Family and domain databases

InterProiIPR001188. Sperm_putr-bd.
[Graphical view]
PIRSFiPIRSF019574. Periplasmic_polyamine_BP. 1 hit.
PRINTSiPR00909. SPERMDNBNDNG.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9I6J0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQHSIGKTLL VAALATAIAG PVQAEKKSLH IYNWTDYIAP TTLKDFTKES
60 70 80 90 100
GIDVSYDVFD SNETLEGKLV SGHSGYDIVV PSNNFLGKQI QAGAFQKLDK
110 120 130 140 150
SKLPNWKNLD PALLKQLEVS DPGNQYAVPY LWGTNGIGYN VAKVKEVLGD
160 170 180 190 200
QPIDSWAILF EPENMKKLAK CGVAFMDSGD EMLPAALNYL GLDPNTHDPK
210 220 230 240 250
DYKKAEEVLT KVRPYVSYFH SSKYISDLAN GNICVAFGYS GDVFQAAARA
260 270 280 290 300
EEAGKGIDIQ YVIPKEGANL WFDLMAIPAD AKAADNAYAF IDYLLRPEVI
310 320 330 340 350
AKVSDYVGYA NAIPGARPLM DKSVSDSEEV YPPQAVLDKL YVSAVLPAKV
360
LRLQTRTWTR IKTGK
Length:365
Mass (Da):40,070
Last modified:March 1, 2001 - v1
Checksum:i3F14A63C3C1D417D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03690.1.
PIRiF83607.
RefSeqiNP_248992.1. NC_002516.2.
WP_003084301.1. NZ_ASJY01000065.1.

Genome annotation databases

EnsemblBacteriaiAAG03690; AAG03690; PA0301.
GeneIDi877626.
KEGGipae:PA0301.
PATRICi19834828. VBIPseAer58763_0315.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03690.1.
PIRiF83607.
RefSeqiNP_248992.1. NC_002516.2.
WP_003084301.1. NZ_ASJY01000065.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3TTLX-ray2.30A/B28-362[»]
3TTNX-ray2.00A/B28-362[»]
ProteinModelPortaliQ9I6J0.
SMRiQ9I6J0. Positions 28-362.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0301.

Proteomic databases

PaxDbiQ9I6J0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG03690; AAG03690; PA0301.
GeneIDi877626.
KEGGipae:PA0301.
PATRICi19834828. VBIPseAer58763_0315.

Organism-specific databases

PseudoCAPiPA0301.

Phylogenomic databases

eggNOGiENOG4108HM4. Bacteria.
COG0687. LUCA.
HOGENOMiHOG000263815.
KOiK11073.
OMAiSVYPPQA.
PhylomeDBiQ9I6J0.

Family and domain databases

InterProiIPR001188. Sperm_putr-bd.
[Graphical view]
PIRSFiPIRSF019574. Periplasmic_polyamine_BP. 1 hit.
PRINTSiPR00909. SPERMDNBNDNG.
ProtoNetiSearch...

Entry informationi

Entry nameiSPUE_PSEAE
AccessioniPrimary (citable) accession number: Q9I6J0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 7, 2015
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.