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Protein

Thymidylate synthase

Gene

thyA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Provides the sole de novo source of dTMP for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei146 – 1461UniRule annotation

GO - Molecular functioni

  1. thymidylate synthase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. dTMP biosynthetic process Source: UniProtKB-HAMAP
  2. dTTP biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:PA0342
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA0342.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 264264Thymidylate synthasePRO_0000141004Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA0342.

Structurei

3D structure databases

ProteinModelPortaliQ9I6F1.
SMRiQ9I6F1. Positions 1-251.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0207.
HOGENOMiHOG000257900.
InParanoidiQ9I6F1.
KOiK00560.
OMAiNEWADEN.
OrthoDBiEOG6K6V53.
PhylomeDBiQ9I6F1.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9I6F1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQYLDLMRH VREHGTFKSD RTGTGTYSVF GHQMRFDLAA GFPLVTTKKC
60 70 80 90 100
HLKSIVHELL WFLKGSTNIA YLKEHGVSIW DEWADENGDL GPVYGYQWRS
110 120 130 140 150
WPAPDGRHID QIANLMAMLK KNPDSRRLIV SAWNPALIDE MALPPCHALF
160 170 180 190 200
QFYVADGKLS CQLYQRSADI FLGVPFNIAS YALLTLMVAQ VAGLQPGEFI
210 220 230 240 250
WTGGDCHLYA NHLEQADLQL TREPLPLPSM KLNPEVKDLF DFRFEDFELV
260
GYEAHPHIKA PVAV
Length:264
Mass (Da):30,016
Last modified:March 1, 2001 - v1
Checksum:iD34075691D06F352
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03731.1.
PIRiD83602.
RefSeqiNP_249033.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG03731; AAG03731; PA0342.
GeneIDi879417.
KEGGipae:PA0342.
PATRICi19834918. VBIPseAer58763_0360.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03731.1.
PIRiD83602.
RefSeqiNP_249033.1. NC_002516.2.

3D structure databases

ProteinModelPortaliQ9I6F1.
SMRiQ9I6F1. Positions 1-251.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0342.

Protocols and materials databases

DNASUi879417.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG03731; AAG03731; PA0342.
GeneIDi879417.
KEGGipae:PA0342.
PATRICi19834918. VBIPseAer58763_0360.

Organism-specific databases

PseudoCAPiPA0342.

Phylogenomic databases

eggNOGiCOG0207.
HOGENOMiHOG000257900.
InParanoidiQ9I6F1.
KOiK00560.
OMAiNEWADEN.
OrthoDBiEOG6K6V53.
PhylomeDBiQ9I6F1.

Enzyme and pathway databases

UniPathwayiUPA00575.

Family and domain databases

Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact.
InterProiIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamiPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.
PROSITEiPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiTYSY_PSEAE
AccessioniPrimary (citable) accession number: Q9I6F1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: March 1, 2001
Last modified: January 7, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.