Reviewed, UniProtKB/Swiss-Prot Q9I6C8 (CALB_PSEAE)

Names and origin

Protein names

Recommended name:
    Probable coniferyl aldehyde dehydrogenase
      Short name=CALDH
    EC=1.2.1.68

Gene names

Name:	calB
Ordered Locus Names:	PA0366

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Coniferyl aldehyde + H2O + NAD(P)+ = ferulate + NAD(P)H.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular aldehyde metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aldehyde dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: InterPro coniferyl-aldehyde dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	Probable coniferyl aldehyde dehydrogenase		PRO_0000056587
Sites
	Active site	225	1	By similarity
	Active site	259	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I6C8-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 9C4A0B4D3E334BDD	FASTA	476	52,876
	10 20 30 40 50 60 MVADIAYLQQ SQQEINQLEA LFARQRAAYL AQPMPDATQR VQWLKALRDL LFKEQQALIE 70 80 90 100 110 120 AIDRDFSARS ADETLLAEIM PSLHGIHYAA KRVKKWMKPA RRAVGLQFQP ASAQVVYQPL 130 140 150 160 170 180 GVVGVIVPWN YPLFLSIGPL TGALAAGNRV MIKMSESTPA TGRLLKDLLA RIFPEDQVAV 190 200 210 220 230 240 VLGEVDVGVA FSKLPFDHLL FTGATSVGKH VMRAAAENLT PVTLELGGKS PAIVSDSVPM 250 260 270 280 290 300 KDAAERIAFG KSLNAGQTCV APDYVLVPSR RVEEFVSQYK EVVQGFFPRL SDNPDYTAII 310 320 330 340 350 360 NERQLGRLRG YLDDAREKGA TLVPLFAEGQ QRRLPQTLLL NVSDDMKVMQ EEIFGPLLPV 370 380 390 400 410 420 IPYERLEDAL AYVNQRPRPL ALYYFGYDKA QQQRVLHETH SGGVCLNDTL LHVAQDDIPF 430 440 450 460 470 GGVGPSGMGH YHGHEGFLTF SKAKGVFSKP RFNAARMIYP PYGKSIQKLV YKLFVR

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG03755.1.
PIR	D83600.
RefSeq	NP_249057.1.
3D structure databases
SMR	Q9I6C8. Positions 22-462.
ModBase	Search...
Genome annotation databases
GeneID	880299.
GenomeReviews	Gene locus PA0366 in contig AE004091_GR.
KEGG	pae:PA0366.
NMPDR	fig|208964.1.peg.367.
Organism-specific databases
PseudoCAP	PA0366.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG752218.
OMA	ALRHGKR.
Enzyme and pathway databases
BioCyc	PAER208964:PA0366-MONOMER.
BRENDA	1.2.1.68. 354.
Family and domain databases
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR012394. Aldehyde_DH_NAD(P). [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF15. ALDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF036492. ALDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. False negative. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CALB_PSEAE

Accession

Primary (citable) accession number: Q9I6C8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 11, 2002
Last sequence update:	March 1, 2001
Last modified:	January 19, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)