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Protein

Thiazole synthase

Gene

thiG

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei107Schiff-base intermediate with DXPUniRule annotation1
Binding sitei168DXP; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:PA0381
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0381.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001628451 – 265Thiazole synthaseAdd BLAST265

Proteomic databases

PaxDbiQ9I6B4.
PRIDEiQ9I6B4.

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA0381.

Structurei

Secondary structure

1265
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 13Combined sources3
Beta strandi16 – 19Combined sources4
Beta strandi22 – 24Combined sources3
Beta strandi29 – 31Combined sources3
Helixi32 – 41Combined sources10
Beta strandi45 – 50Combined sources6
Helixi51 – 53Combined sources3
Turni71 – 73Combined sources3
Beta strandi74 – 79Combined sources6
Helixi86 – 97Combined sources12
Turni98 – 101Combined sources4
Beta strandi105 – 108Combined sources4
Turni114 – 116Combined sources3
Helixi121 – 132Combined sources12
Turni133 – 135Combined sources3
Beta strandi137 – 142Combined sources6
Helixi146 – 154Combined sources9
Beta strandi158 – 162Combined sources5
Beta strandi164 – 166Combined sources3
Helixi176 – 185Combined sources10
Beta strandi190 – 194Combined sources5
Helixi199 – 208Combined sources10
Beta strandi211 – 216Combined sources6
Helixi217 – 220Combined sources4
Beta strandi222 – 224Combined sources3
Helixi225 – 245Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WV2X-ray2.90A/B1-265[»]
ProteinModelPortaliQ9I6B4.
SMRiQ9I6B4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9I6B4.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni194 – 195DXP bindingUniRule annotation2
Regioni216 – 217DXP bindingUniRule annotation2

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA8. Bacteria.
COG2022. LUCA.
InParanoidiQ9I6B4.
KOiK03149.
OMAiAQYPSPA.
PhylomeDBiQ9I6B4.

Family and domain databases

CDDicd04728. ThiG. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR008867. ThiG.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9I6B4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQASSTDTP FVIAGRTYGS RLLVGTGKYK DLDETRRAIE ASGAEIVTVA
60 70 80 90 100
VRRTNIGQNP DEPNLLDVIP PDRYTILPNT AGCYDAVEAV RTCRLARELL
110 120 130 140 150
DGHNLVKLEV LADQKTLFPN VVETLKAAEQ LVKDGFDVMV YTSDDPIIAR
160 170 180 190 200
QLAEIGCIAV MPLAGLIGSG LGICNPYNLR IILEEAKVPV LVDAGVGTAS
210 220 230 240 250
DAAIAMELGC EAVLMNTAIA HAKDPVMMAE AMKHAIVAGR LAYLAGRMPR
260
KLYASASSPL DGLID
Length:265
Mass (Da):28,235
Last modified:March 1, 2001 - v1
Checksum:iEE3F74F94F0501E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03770.1.
PIRiE83599.
RefSeqiNP_249072.1. NC_002516.2.
WP_003084517.1. NZ_ASJY01000075.1.

Genome annotation databases

EnsemblBacteriaiAAG03770; AAG03770; PA0381.
GeneIDi879802.
KEGGipae:PA0381.
PATRICi19835000. VBIPseAer58763_0401.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03770.1.
PIRiE83599.
RefSeqiNP_249072.1. NC_002516.2.
WP_003084517.1. NZ_ASJY01000075.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WV2X-ray2.90A/B1-265[»]
ProteinModelPortaliQ9I6B4.
SMRiQ9I6B4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0381.

Proteomic databases

PaxDbiQ9I6B4.
PRIDEiQ9I6B4.

Protocols and materials databases

DNASUi879802.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG03770; AAG03770; PA0381.
GeneIDi879802.
KEGGipae:PA0381.
PATRICi19835000. VBIPseAer58763_0401.

Organism-specific databases

PseudoCAPiPA0381.

Phylogenomic databases

eggNOGiENOG4105CA8. Bacteria.
COG2022. LUCA.
InParanoidiQ9I6B4.
KOiK03149.
OMAiAQYPSPA.
PhylomeDBiQ9I6B4.

Enzyme and pathway databases

UniPathwayiUPA00060.

Miscellaneous databases

EvolutionaryTraceiQ9I6B4.

Family and domain databases

CDDicd04728. ThiG. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR008867. ThiG.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHIG_PSEAE
AccessioniPrimary (citable) accession number: Q9I6B4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.