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Protein

Thiazole synthase

Gene

thiG

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei107 – 1071Schiff-base intermediate with DXPUniRule annotation
Binding sitei168 – 1681DXP; via amide nitrogenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:PA0381
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0381.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 265265Thiazole synthasePRO_0000162845Add
BLAST

Proteomic databases

PaxDbiQ9I6B4.

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA0381.

Structurei

Secondary structure

1
265
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 133Combined sources
Beta strandi16 – 194Combined sources
Beta strandi22 – 243Combined sources
Beta strandi29 – 313Combined sources
Helixi32 – 4110Combined sources
Beta strandi45 – 506Combined sources
Helixi51 – 533Combined sources
Turni71 – 733Combined sources
Beta strandi74 – 796Combined sources
Helixi86 – 9712Combined sources
Turni98 – 1014Combined sources
Beta strandi105 – 1084Combined sources
Turni114 – 1163Combined sources
Helixi121 – 13212Combined sources
Turni133 – 1353Combined sources
Beta strandi137 – 1426Combined sources
Helixi146 – 1549Combined sources
Beta strandi158 – 1625Combined sources
Beta strandi164 – 1663Combined sources
Helixi176 – 18510Combined sources
Beta strandi190 – 1945Combined sources
Helixi199 – 20810Combined sources
Beta strandi211 – 2166Combined sources
Helixi217 – 2204Combined sources
Beta strandi222 – 2243Combined sources
Helixi225 – 24521Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WV2X-ray2.90A/B1-265[»]
ProteinModelPortaliQ9I6B4.
SMRiQ9I6B4. Positions 9-251.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9I6B4.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni194 – 1952DXP bindingUniRule annotation
Regioni216 – 2172DXP bindingUniRule annotation

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA8. Bacteria.
COG2022. LUCA.
InParanoidiQ9I6B4.
KOiK03149.
OMAiAQYPSPA.
OrthoDBiEOG6KMBD9.
PhylomeDBiQ9I6B4.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG.
InterProiIPR013785. Aldolase_TIM.
IPR008867. ThiG.
[Graphical view]
SUPFAMiSSF110399. SSF110399. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9I6B4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQASSTDTP FVIAGRTYGS RLLVGTGKYK DLDETRRAIE ASGAEIVTVA
60 70 80 90 100
VRRTNIGQNP DEPNLLDVIP PDRYTILPNT AGCYDAVEAV RTCRLARELL
110 120 130 140 150
DGHNLVKLEV LADQKTLFPN VVETLKAAEQ LVKDGFDVMV YTSDDPIIAR
160 170 180 190 200
QLAEIGCIAV MPLAGLIGSG LGICNPYNLR IILEEAKVPV LVDAGVGTAS
210 220 230 240 250
DAAIAMELGC EAVLMNTAIA HAKDPVMMAE AMKHAIVAGR LAYLAGRMPR
260
KLYASASSPL DGLID
Length:265
Mass (Da):28,235
Last modified:March 1, 2001 - v1
Checksum:iEE3F74F94F0501E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03770.1.
PIRiE83599.
RefSeqiNP_249072.1. NC_002516.2.
WP_003084517.1. NZ_ASJY01000075.1.

Genome annotation databases

EnsemblBacteriaiAAG03770; AAG03770; PA0381.
GeneIDi879802.
KEGGipae:PA0381.
PATRICi19835000. VBIPseAer58763_0401.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03770.1.
PIRiE83599.
RefSeqiNP_249072.1. NC_002516.2.
WP_003084517.1. NZ_ASJY01000075.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WV2X-ray2.90A/B1-265[»]
ProteinModelPortaliQ9I6B4.
SMRiQ9I6B4. Positions 9-251.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0381.

Proteomic databases

PaxDbiQ9I6B4.

Protocols and materials databases

DNASUi879802.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG03770; AAG03770; PA0381.
GeneIDi879802.
KEGGipae:PA0381.
PATRICi19835000. VBIPseAer58763_0401.

Organism-specific databases

PseudoCAPiPA0381.

Phylogenomic databases

eggNOGiENOG4105CA8. Bacteria.
COG2022. LUCA.
InParanoidiQ9I6B4.
KOiK03149.
OMAiAQYPSPA.
OrthoDBiEOG6KMBD9.
PhylomeDBiQ9I6B4.

Enzyme and pathway databases

UniPathwayiUPA00060.

Miscellaneous databases

EvolutionaryTraceiQ9I6B4.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG.
InterProiIPR013785. Aldolase_TIM.
IPR008867. ThiG.
[Graphical view]
SUPFAMiSSF110399. SSF110399. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. "Crystal structure of thiamine biosynthesis protein from Pseudomonas aeruginosa."
    New York structural genomics research consortium (NYSGRC)
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).

Entry informationi

Entry nameiTHIG_PSEAE
AccessioniPrimary (citable) accession number: Q9I6B4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: March 1, 2001
Last modified: July 6, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.