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Q9I6B3

- TRMB_PSEAE

UniProt

Q9I6B3 - TRMB_PSEAE

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
trmB, PA0382
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei74 – 741S-adenosyl-L-methionine By similarity
Binding sitei99 – 991S-adenosyl-L-methionine By similarity
Binding sitei126 – 1261S-adenosyl-L-methionine By similarity
Active sitei149 – 1491 By similarity
Binding sitei149 – 1491S-adenosyl-L-methionine By similarity
Binding sitei153 – 1531Substrate By similarity
Binding sitei185 – 1851Substrate By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:trmB
Ordered Locus Names:PA0382
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA0382.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 244244tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
PRO_0000171376Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi208964.PA0382.

Structurei

3D structure databases

ProteinModelPortaliQ9I6B3.
SMRiQ9I6B3. Positions 47-243.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni222 – 2254Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0220.
KOiK03439.
OMAiHSTLEMA.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9I6B3-1 [UniParc]FASTAAdd to Basket

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MSDTPQSPAQ DSLAEHDEAR PMRTVKSFVM RAGRMTEGQQ RGLDLGWPKF    50
GLELSDEVQD FDAIFGRQAP RTFEIGFGMG HSTLEMAAAA PDIDFIGVEV 100
HKPGVGALLN GLLTQGLGNV RVYSCDALEV LRHCVADASL DRLLLFFPDP 150
WHKKRHHKRR IVQPEFAELV RRKLKVGGVL HMATDWEPYA EHMLDVMSAA 200
PGYRNQAEDG RFVPRPQERP VTKFERRGER LGHGVWDLKF ERVD 244
Length:244
Mass (Da):27,595
Last modified:June 1, 2001 - v2
Checksum:i4D103EB6F5A1B232
GO

Sequence cautioni

The sequence AAG03771.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004091 Genomic DNA. Translation: AAG03771.1. Different initiation.
PIRiE83596.
RefSeqiNP_249073.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG03771; AAG03771; PA0382.
GeneIDi879787.
KEGGipae:PA0382.
PATRICi19835002. VBIPseAer58763_0402.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE004091 Genomic DNA. Translation: AAG03771.1 . Different initiation.
PIRi E83596.
RefSeqi NP_249073.1. NC_002516.2.

3D structure databases

ProteinModelPortali Q9I6B3.
SMRi Q9I6B3. Positions 47-243.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 208964.PA0382.

Protocols and materials databases

DNASUi 879787.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG03771 ; AAG03771 ; PA0382 .
GeneIDi 879787.
KEGGi pae:PA0382.
PATRICi 19835002. VBIPseAer58763_0402.

Organism-specific databases

PseudoCAPi PA0382.

Phylogenomic databases

eggNOGi COG0220.
KOi K03439.
OMAi HSTLEMA.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiTRMB_PSEAE
AccessioniPrimary (citable) accession number: Q9I6B3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: June 11, 2014
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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