Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9I6B3 (TRMB_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:PA0382
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length244 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Sequence caution

The sequence AAG03771.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 244244tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171376

Regions

Region222 – 2254Substrate binding By similarity

Sites

Active site1491 By similarity
Binding site741S-adenosyl-L-methionine By similarity
Binding site991S-adenosyl-L-methionine By similarity
Binding site1261S-adenosyl-L-methionine By similarity
Binding site1491S-adenosyl-L-methionine By similarity
Binding site1531Substrate By similarity
Binding site1851Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9I6B3 [UniParc].

Last modified June 1, 2001. Version 2.
Checksum: 4D103EB6F5A1B232

FASTA24427,595
        10         20         30         40         50         60 
MSDTPQSPAQ DSLAEHDEAR PMRTVKSFVM RAGRMTEGQQ RGLDLGWPKF GLELSDEVQD 

        70         80         90        100        110        120 
FDAIFGRQAP RTFEIGFGMG HSTLEMAAAA PDIDFIGVEV HKPGVGALLN GLLTQGLGNV 

       130        140        150        160        170        180 
RVYSCDALEV LRHCVADASL DRLLLFFPDP WHKKRHHKRR IVQPEFAELV RRKLKVGGVL 

       190        200        210        220        230        240 
HMATDWEPYA EHMLDVMSAA PGYRNQAEDG RFVPRPQERP VTKFERRGER LGHGVWDLKF 


ERVD 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE004091 Genomic DNA. Translation: AAG03771.1. Different initiation.
PIRE83596.
RefSeqNP_249073.1. NC_002516.2.

3D structure databases

ProteinModelPortalQ9I6B3.
SMRQ9I6B3. Positions 47-243.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING208964.PA0382.

Protocols and materials databases

DNASU879787.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID879787.
KEGGpae:PA0382.
PATRIC19835002. VBIPseAer58763_0402.

Organism-specific databases

PseudoCAPPA0382.

Phylogenomic databases

eggNOGCOG0220.
KOK03439.
OMADNYWPVM.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_PSEAE
AccessionPrimary (citable) accession number: Q9I6B3
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways