Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9I6B3

- TRMB_PSEAE

UniProt

Q9I6B3 - TRMB_PSEAE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei74 – 741S-adenosyl-L-methionineUniRule annotation
Binding sitei99 – 991S-adenosyl-L-methionineUniRule annotation
Binding sitei126 – 1261S-adenosyl-L-methionineUniRule annotation
Active sitei149 – 1491By similarity
Binding sitei149 – 1491S-adenosyl-L-methionineUniRule annotation
Binding sitei153 – 1531SubstrateUniRule annotation
Binding sitei185 – 1851SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:PA0382
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438: Chromosome

Organism-specific databases

PseudoCAPiPA0382.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 244244tRNA (guanine-N(7)-)-methyltransferasePRO_0000171376Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi208964.PA0382.

Structurei

3D structure databases

ProteinModelPortaliQ9I6B3.
SMRiQ9I6B3. Positions 47-243.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni222 – 2254Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
InParanoidiQ9I6B3.
KOiK03439.
OMAiHSTLEMA.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9I6B3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDTPQSPAQ DSLAEHDEAR PMRTVKSFVM RAGRMTEGQQ RGLDLGWPKF
60 70 80 90 100
GLELSDEVQD FDAIFGRQAP RTFEIGFGMG HSTLEMAAAA PDIDFIGVEV
110 120 130 140 150
HKPGVGALLN GLLTQGLGNV RVYSCDALEV LRHCVADASL DRLLLFFPDP
160 170 180 190 200
WHKKRHHKRR IVQPEFAELV RRKLKVGGVL HMATDWEPYA EHMLDVMSAA
210 220 230 240
PGYRNQAEDG RFVPRPQERP VTKFERRGER LGHGVWDLKF ERVD
Length:244
Mass (Da):27,595
Last modified:June 1, 2001 - v2
Checksum:i4D103EB6F5A1B232
GO

Sequence cautioni

The sequence AAG03771.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03771.1. Different initiation.
PIRiE83596.
RefSeqiNP_249073.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG03771; AAG03771; PA0382.
GeneIDi879787.
KEGGipae:PA0382.
PATRICi19835002. VBIPseAer58763_0402.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03771.1 . Different initiation.
PIRi E83596.
RefSeqi NP_249073.1. NC_002516.2.

3D structure databases

ProteinModelPortali Q9I6B3.
SMRi Q9I6B3. Positions 47-243.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 208964.PA0382.

Protocols and materials databases

DNASUi 879787.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG03771 ; AAG03771 ; PA0382 .
GeneIDi 879787.
KEGGi pae:PA0382.
PATRICi 19835002. VBIPseAer58763_0402.

Organism-specific databases

PseudoCAPi PA0382.

Phylogenomic databases

eggNOGi COG0220.
InParanoidi Q9I6B3.
KOi K03439.
OMAi HSTLEMA.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiTRMB_PSEAE
AccessioniPrimary (citable) accession number: Q9I6B3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: October 29, 2014
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3