Q9I6B3 (TRMB_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	PA0382

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.
Sequence caution	The sequence AAG03771.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057		PRO_0000171376
Regions
	Region	222 – 225	4	Substrate binding Potential
Sites
	Binding site	74	1	S-adenosyl-L-methionine By similarity
	Binding site	99	1	S-adenosyl-L-methionine By similarity
	Binding site	126	1	S-adenosyl-L-methionine By similarity
	Binding site	149	1	S-adenosyl-L-methionine By similarity
	Binding site	153	1	Substrate By similarity
	Binding site	185	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9I6B3 [UniParc]. Last modified June 1, 2001. Version 2. Checksum: 4D103EB6F5A1B232	FASTA	244	27,595
	10 20 30 40 50 60 MSDTPQSPAQ DSLAEHDEAR PMRTVKSFVM RAGRMTEGQQ RGLDLGWPKF GLELSDEVQD 70 80 90 100 110 120 FDAIFGRQAP RTFEIGFGMG HSTLEMAAAA PDIDFIGVEV HKPGVGALLN GLLTQGLGNV 130 140 150 160 170 180 RVYSCDALEV LRHCVADASL DRLLLFFPDP WHKKRHHKRR IVQPEFAELV RRKLKVGGVL 190 200 210 220 230 240 HMATDWEPYA EHMLDVMSAA PGYRNQAEDG RFVPRPQERP VTKFERRGER LGHGVWDLKF ERVD

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG03771.1. Different initiation.
PIR	E83596.
RefSeq	NP_249073.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9I6B3.
SMR	Q9I6B3. Positions 47-243.
ModBase	Search...
Protein-protein interaction databases
STRING	208964.PA0382.
Protocols and materials databases
DNASU	879787.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	879787.
KEGG	pae:PA0382.
PATRIC	19835002. VBIPseAer58763_0402.
Organism-specific databases
PseudoCAP	PA0382.
Phylogenomic databases
eggNOG	COG0220.
KO	K03439.
OMA	CEVHEPG.
ProtClustDB	PRK00121.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
HAMAP	MF_01057. tRNA_methyltr_TrmB.
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_PSEAE

Accession

Primary (citable) accession number: Q9I6B3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	June 1, 2001
Last modified:	May 1, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program