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Protein

Non-canonical purine NTP pyrophosphatase

Gene

PA0387

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions.UniRule annotation

Catalytic activityi

A nucleoside triphosphate + H2O = a nucleotide + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 divalent metal cation per subunit; can use either Mg2+ or Mn2+.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi42 – 421Magnesium or manganeseUniRule annotation
Metal bindingi71 – 711Magnesium or manganeseUniRule annotation
Binding sitei158 – 1581SubstrateUniRule annotation
Binding sitei178 – 1781SubstrateUniRule annotation
Binding sitei184 – 1841SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Nucleotide metabolism

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Non-canonical purine NTP pyrophosphataseUniRule annotation (EC:3.6.1.19UniRule annotation)
Alternative name(s):
Non-standard purine NTP pyrophosphataseUniRule annotation
Nucleoside-triphosphate diphosphataseUniRule annotation
Nucleoside-triphosphate pyrophosphataseUniRule annotation
Short name:
NTPaseUniRule annotation
Gene namesi
Ordered Locus Names:PA0387
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0387.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 197197Non-canonical purine NTP pyrophosphatasePRO_0000178213Add
BLAST

Proteomic databases

PaxDbiQ9I6A8.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA0387.

Structurei

3D structure databases

ProteinModelPortaliQ9I6A8.
SMRiQ9I6A8. Positions 4-191.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni11 – 166Substrate bindingUniRule annotation
Regioni71 – 722Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the HAM1 NTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108V82. Bacteria.
COG0127. LUCA.
HOGENOMiHOG000293319.
InParanoidiQ9I6A8.
KOiK02428.
OMAiCEGLWHG.
OrthoDBiEOG6CZQQP.
PhylomeDBiQ9I6A8.

Family and domain databases

Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase.
InterProiIPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
IPR020922. NTPase.
[Graphical view]
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiPF01725. Ham1p_like. 1 hit.
[Graphical view]
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9I6A8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKLEQLVLA SHNAGKLKEL QAMLGASVKV RSIGEFSQVE PEETGLSFVE
60 70 80 90 100
NAILKARNAA RLSGLPALAD DSGLAVDFLG GAPGIYSARY ADGRGDAANN
110 120 130 140 150
AKLLEAMKDV PDAERGAQFV SVLALVRHAD DPLPILCEGI WEGRILREAR
160 170 180 190
GAHGFGYDPL FWVPERDCSS AELAPEEKNR LSHRARAMAL LKQRLGL
Length:197
Mass (Da):21,242
Last modified:March 1, 2001 - v1
Checksum:i778471EE9BD53531
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03776.1.
PIRiB83597.
RefSeqiNP_249078.1. NC_002516.2.
WP_003084527.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG03776; AAG03776; PA0387.
GeneIDi878675.
KEGGipae:PA0387.
PATRICi19835012. VBIPseAer58763_0407.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG03776.1.
PIRiB83597.
RefSeqiNP_249078.1. NC_002516.2.
WP_003084527.1. NC_002516.2.

3D structure databases

ProteinModelPortaliQ9I6A8.
SMRiQ9I6A8. Positions 4-191.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0387.

Proteomic databases

PaxDbiQ9I6A8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG03776; AAG03776; PA0387.
GeneIDi878675.
KEGGipae:PA0387.
PATRICi19835012. VBIPseAer58763_0407.

Organism-specific databases

PseudoCAPiPA0387.

Phylogenomic databases

eggNOGiENOG4108V82. Bacteria.
COG0127. LUCA.
HOGENOMiHOG000293319.
InParanoidiQ9I6A8.
KOiK02428.
OMAiCEGLWHG.
OrthoDBiEOG6CZQQP.
PhylomeDBiQ9I6A8.

Family and domain databases

Gene3Di3.90.950.10. 1 hit.
HAMAPiMF_01405. Non_canon_purine_NTPase.
InterProiIPR002637. Ham1p-like.
IPR029001. ITPase-like_fam.
IPR020922. NTPase.
[Graphical view]
PANTHERiPTHR11067. PTHR11067. 1 hit.
PfamiPF01725. Ham1p_like. 1 hit.
[Graphical view]
SUPFAMiSSF52972. SSF52972. 1 hit.
TIGRFAMsiTIGR00042. TIGR00042. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiNTPA_PSEAE
AccessioniPrimary (citable) accession number: Q9I6A8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: March 1, 2001
Last modified: November 11, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.