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Protein

dITP/XTP pyrophosphatase

Gene

PA0387

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.UniRule annotation

Catalytic activityi

XTP + H2O = XMP + diphosphate.UniRule annotation
dITP + H2O = dIMP + diphosphate.UniRule annotation
ITP + H2O = IMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi42MagnesiumUniRule annotation1
Active sitei71Proton acceptorUniRule annotation1
Metal bindingi71MagnesiumUniRule annotation1
Binding sitei72Substrate; via amide nitrogenUniRule annotation1
Binding sitei178SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPAER208964:G1FZ6-391-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
dITP/XTP pyrophosphataseUniRule annotation (EC:3.6.1.66UniRule annotation)
Alternative name(s):
Non-canonical purine NTP pyrophosphataseUniRule annotation
Non-standard purine NTP pyrophosphataseUniRule annotation
Nucleoside-triphosphate diphosphataseUniRule annotation
Nucleoside-triphosphate pyrophosphataseUniRule annotation
Short name:
NTPaseUniRule annotation
Gene namesi
Ordered Locus Names:PA0387
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0387

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001782131 – 197dITP/XTP pyrophosphataseAdd BLAST197

Proteomic databases

PaxDbiQ9I6A8

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA0387

Structurei

3D structure databases

ProteinModelPortaliQ9I6A8
SMRiQ9I6A8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni11 – 16Substrate bindingUniRule annotation6
Regioni155 – 158Substrate bindingUniRule annotation4
Regioni183 – 184Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the HAM1 NTPase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108V82 Bacteria
COG0127 LUCA
HOGENOMiHOG000293319
InParanoidiQ9I6A8
KOiK02428
OMAiYDPIFQP
PhylomeDBiQ9I6A8

Family and domain databases

CDDicd00515 HAM1, 1 hit
Gene3Di3.90.950.10, 1 hit
HAMAPiMF_01405 Non_canon_purine_NTPase, 1 hit
InterProiView protein in InterPro
IPR020922 dITP/XTP_pyrophosphatase
IPR002637 Ham1p-like
IPR029001 ITPase-like_fam
PANTHERiPTHR11067 PTHR11067, 1 hit
PfamiView protein in Pfam
PF01725 Ham1p_like, 1 hit
SUPFAMiSSF52972 SSF52972, 1 hit
TIGRFAMsiTIGR00042 TIGR00042, 1 hit

Sequencei

Sequence statusi: Complete.

Q9I6A8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKLEQLVLA SHNAGKLKEL QAMLGASVKV RSIGEFSQVE PEETGLSFVE
60 70 80 90 100
NAILKARNAA RLSGLPALAD DSGLAVDFLG GAPGIYSARY ADGRGDAANN
110 120 130 140 150
AKLLEAMKDV PDAERGAQFV SVLALVRHAD DPLPILCEGI WEGRILREAR
160 170 180 190
GAHGFGYDPL FWVPERDCSS AELAPEEKNR LSHRARAMAL LKQRLGL
Length:197
Mass (Da):21,242
Last modified:March 1, 2001 - v1
Checksum:i778471EE9BD53531
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA Translation: AAG03776.1
PIRiB83597
RefSeqiNP_249078.1, NC_002516.2
WP_003084527.1, NC_002516.2

Genome annotation databases

EnsemblBacteriaiAAG03776; AAG03776; PA0387
GeneIDi878675
KEGGipae:PA0387
PATRICifig|208964.12.peg.407

Similar proteinsi

Entry informationi

Entry nameiIXTPA_PSEAE
AccessioniPrimary (citable) accession number: Q9I6A8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: March 1, 2001
Last modified: May 23, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health