Q9I6A8 (NTPA_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

PA0387

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 197	197	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178213
Regions
	Region	11 – 16	6	Substrate binding By similarity
	Region	71 – 72	2	Substrate binding By similarity
Sites
	Metal binding	42	1	Magnesium or manganese By similarity
	Metal binding	71	1	Magnesium or manganese By similarity
	Binding site	158	1	Substrate By similarity
	Binding site	178	1	Substrate By similarity
	Binding site	184	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I6A8 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 778471EE9BD53531	FASTA	197	21,242
	10 20 30 40 50 60 MIKLEQLVLA SHNAGKLKEL QAMLGASVKV RSIGEFSQVE PEETGLSFVE NAILKARNAA 70 80 90 100 110 120 RLSGLPALAD DSGLAVDFLG GAPGIYSARY ADGRGDAANN AKLLEAMKDV PDAERGAQFV 130 140 150 160 170 180 SVLALVRHAD DPLPILCEGI WEGRILREAR GAHGFGYDPL FWVPERDCSS AELAPEEKNR 190 LSHRARAMAL LKQRLGL

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG03776.1.
PIR	B83597.
RefSeq	NP_249078.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9I6A8.
SMR	Q9I6A8. Positions 4-195.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	878675.
GenomeReviews	Gene locus PA0387 in contig AE004091_GR.
KEGG	pae:PA0387.
PATRIC	19835012. VBIPseAer58763_0407.
Organism-specific databases
PseudoCAP	PA0387.
Phylogenomic databases
HOGENOM	HBG697237.
OMA	YSKRYDQ.
ProtClustDB	PRK00120.
Enzyme and pathway databases
BioCyc	PAER208964:PA0387-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_PSEAE

Accession

Primary (citable) accession number: Q9I6A8

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2002
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program