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Q9I697

- GSHB_PSEAE

UniProt

Q9I697 - GSHB_PSEAE

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Protein
Glutathione synthetase
Gene
gshB, PA0407
Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi282 – 2821Magnesium or manganese By similarity
Metal bindingi284 – 2841Magnesium or manganese By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi152 – 20857ATP By similarity
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutathione synthase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. manganese ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Glutathione biosynthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00142; UER00210.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione synthetase (EC:6.3.2.3)
    Alternative name(s):
    GSH synthetase
    Short name:
    GSH-S
    Short name:
    GSHase
    Glutathione synthase
    Gene namesi
    Name:gshB
    Ordered Locus Names:PA0407
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA0407.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: InterPro
    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 317317Glutathione synthetaseUniRule annotation
    PRO_0000197476Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi208964.PA0407.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9I697.
    SMRiQ9I697. Positions 3-314.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini126 – 311186ATP-grasp
    Add
    BLAST

    Sequence similaritiesi

    Contains 1 ATP-grasp domain.

    Phylogenomic databases

    eggNOGiCOG0189.
    KOiK01920.
    OMAiDWKIARA.
    OrthoDBiEOG6WMHWB.
    PhylomeDBiQ9I697.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    HAMAPiMF_00162. GSH_S.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR006284. Glut_synth_pro.
    IPR004218. GSHS_ATP-bd.
    IPR004215. GSHS_N.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PfamiPF02955. GSH-S_ATP. 1 hit.
    PF02951. GSH-S_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01380. glut_syn. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9I697-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVRLGIVMD PIARINFKKD SSLAMLLAAQ ARGWSLFYME QQDLYQKAGV    50
    ARGRMRPLKV FNDASRWFEL EAESDQPLHE LDVILMRKDP PFDNEFVYST 100
    YLLEQAERAG ALVVNRPQSL RDCNEKFFAT QFTQCTPPTM VSRRSDILRE 150
    FAAEHRDIIL KPLDGMGGSS IFRHREGDPN LSVILETLTQ HGSQQIMAQR 200
    YLPEIKDGDK RILMIDGEPV PYCLARIPAQ GETRGNLAAG GRGVAQPLSE 250
    RDRWIAAEVG PHLRERGLLF VGLDVIGDYL TEINVTSPTC IREIDQAFDT 300
    RIGDKLMDAI AAQLAAR 317
    Length:317
    Mass (Da):35,705
    Last modified:March 1, 2001 - v1
    Checksum:i543E960203929754
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG03796.1.
    PIRiG83593.
    RefSeqiNP_249098.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG03796; AAG03796; PA0407.
    GeneIDi878223.
    KEGGipae:PA0407.
    PATRICi19835054. VBIPseAer58763_0428.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG03796.1 .
    PIRi G83593.
    RefSeqi NP_249098.1. NC_002516.2.

    3D structure databases

    ProteinModelPortali Q9I697.
    SMRi Q9I697. Positions 3-314.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 208964.PA0407.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG03796 ; AAG03796 ; PA0407 .
    GeneIDi 878223.
    KEGGi pae:PA0407.
    PATRICi 19835054. VBIPseAer58763_0428.

    Organism-specific databases

    PseudoCAPi PA0407.

    Phylogenomic databases

    eggNOGi COG0189.
    KOi K01920.
    OMAi DWKIARA.
    OrthoDBi EOG6WMHWB.
    PhylomeDBi Q9I697.

    Enzyme and pathway databases

    UniPathwayi UPA00142 ; UER00210 .

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    HAMAPi MF_00162. GSH_S.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR006284. Glut_synth_pro.
    IPR004218. GSHS_ATP-bd.
    IPR004215. GSHS_N.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    Pfami PF02955. GSH-S_ATP. 1 hit.
    PF02951. GSH-S_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR01380. glut_syn. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

    Entry informationi

    Entry nameiGSHB_PSEAE
    AccessioniPrimary (citable) accession number: Q9I697
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 23, 2002
    Last sequence update: March 1, 2001
    Last modified: July 9, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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