Q9I697 (GSHB_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione synthetase
EC=6.3.2.3

Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase

Gene names

Name:	gshB
Ordered Locus Names:	PA0407

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162
Sequence similarities	Belongs to the prokaryotic GSH synthase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytosol Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP glutathione synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Glutathione synthetase HAMAP-Rule MF_00162		PRO_0000197476
Regions
	Domain	126 – 311	186	ATP-grasp
	Nucleotide binding	152 – 208	57	ATP By similarity
Sites
	Metal binding	282	1	Magnesium or manganese By similarity
	Metal binding	284	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I697 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 543E960203929754	FASTA	317	35,705
	10 20 30 40 50 60 MSVRLGIVMD PIARINFKKD SSLAMLLAAQ ARGWSLFYME QQDLYQKAGV ARGRMRPLKV 70 80 90 100 110 120 FNDASRWFEL EAESDQPLHE LDVILMRKDP PFDNEFVYST YLLEQAERAG ALVVNRPQSL 130 140 150 160 170 180 RDCNEKFFAT QFTQCTPPTM VSRRSDILRE FAAEHRDIIL KPLDGMGGSS IFRHREGDPN 190 200 210 220 230 240 LSVILETLTQ HGSQQIMAQR YLPEIKDGDK RILMIDGEPV PYCLARIPAQ GETRGNLAAG 250 260 270 280 290 300 GRGVAQPLSE RDRWIAAEVG PHLRERGLLF VGLDVIGDYL TEINVTSPTC IREIDQAFDT 310 RIGDKLMDAI AAQLAAR

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG03796.1.
PIR	G83593.
RefSeq	NP_249098.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9I697.
SMR	Q9I697. Positions 3-314.
ModBase	Search...
Protein-protein interaction databases
STRING	208964.PA0407.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	878223.
KEGG	pae:PA0407.
PATRIC	19835054. VBIPseAer58763_0428.
Organism-specific databases
PseudoCAP	PA0407.
Phylogenomic databases
eggNOG	COG0189.
KO	K01920.
OMA	WMRKDPP.
ProtClustDB	PRK05246.
Enzyme and pathway databases
UniPathway	UPA00142; UER00210.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit.
HAMAP	MF_00162. GSH-S.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR006284. Glut_synth_pro. IPR004218. GSHS_ATP-bd. IPR004215. GSHS_N. IPR016185. PreATP-grasp_dom. [Graphical view]
Pfam	PF02955. GSH-S_ATP. 1 hit. PF02951. GSH-S_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMs	TIGR01380. glut_syn. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHB_PSEAE

Accession

Primary (citable) accession number: Q9I697

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program