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Q9I697 (GSHB_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase

EC=6.3.2.3
Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase
Gene names
Name:gshB
Ordered Locus Names:PA0407
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family.

Contains 1 ATP-grasp domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 317317Glutathione synthetase HAMAP-Rule MF_00162
PRO_0000197476

Regions

Domain126 – 311186ATP-grasp
Nucleotide binding152 – 20857ATP By similarity

Sites

Metal binding2821Magnesium or manganese By similarity
Metal binding2841Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9I697 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 543E960203929754

FASTA31735,705
        10         20         30         40         50         60 
MSVRLGIVMD PIARINFKKD SSLAMLLAAQ ARGWSLFYME QQDLYQKAGV ARGRMRPLKV 

        70         80         90        100        110        120 
FNDASRWFEL EAESDQPLHE LDVILMRKDP PFDNEFVYST YLLEQAERAG ALVVNRPQSL 

       130        140        150        160        170        180 
RDCNEKFFAT QFTQCTPPTM VSRRSDILRE FAAEHRDIIL KPLDGMGGSS IFRHREGDPN 

       190        200        210        220        230        240 
LSVILETLTQ HGSQQIMAQR YLPEIKDGDK RILMIDGEPV PYCLARIPAQ GETRGNLAAG 

       250        260        270        280        290        300 
GRGVAQPLSE RDRWIAAEVG PHLRERGLLF VGLDVIGDYL TEINVTSPTC IREIDQAFDT 

       310 
RIGDKLMDAI AAQLAAR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE004091 Genomic DNA. Translation: AAG03796.1.
PIRG83593.
RefSeqNP_249098.1. NC_002516.2.

3D structure databases

ProteinModelPortalQ9I697.
SMRQ9I697. Positions 3-314.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING208964.PA0407.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG03796; AAG03796; PA0407.
GeneID878223.
KEGGpae:PA0407.
PATRIC19835054. VBIPseAer58763_0428.

Organism-specific databases

PseudoCAPPA0407.

Phylogenomic databases

eggNOGCOG0189.
KOK01920.
OMADWKIARA.
OrthoDBEOG6WMHWB.
PhylomeDBQ9I697.

Enzyme and pathway databases

UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHB_PSEAE
AccessionPrimary (citable) accession number: Q9I697
Entry history
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways