Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9I636

- MASZ_PSEAE

UniProt

Q9I636 - MASZ_PSEAE

Protein

Malate synthase G

Gene

glcB

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei276 – 2761Acetyl-CoAUniRule annotation
    Binding sitei313 – 3131Acetyl-CoAUniRule annotation
    Active sitei340 – 3401Proton acceptorUniRule annotation
    Binding sitei340 – 3401GlyoxylateUniRule annotation
    Metal bindingi432 – 4321MagnesiumUniRule annotation
    Binding sitei432 – 4321GlyoxylateUniRule annotation
    Metal bindingi460 – 4601MagnesiumUniRule annotation
    Binding sitei541 – 5411Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei631 – 6311Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:PA0482
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA0482.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 725725Malate synthase GPRO_0000166891Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei617 – 6171Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi208964.PA0482.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9I636.
    SMRiQ9I636. Positions 2-722.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
    Regioni457 – 4604Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    KOiK01638.
    OMAiSQFIENE.
    OrthoDBiEOG6HJ286.
    PhylomeDBiQ9I636.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9I636-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTERVQVGGL QVAKVLFDFV NNEAIPGTGV SADTFWTGAE AVINDLAPKN    50
    KALLAKRDEL QAKIDGWHQA RAGQAHDAVA YKAFLEEIGY LLPEAEDFQA 100
    GTQNVDDEIA RMAGPQLVVP VMNARFALNA SNARWGSLYD ALYGTDVISE 150
    EGGAEKGKGY NKVRGDKVIA FARAFLDEAA PLESGSHVDA TSYSVKNGAL 200
    VVALKNGSET GLKNAGQFLA FQGDAAKPQA VLLKHNGLHF EIQIDPSSPV 250
    GQTDAAGVKD VLMEAALTTI MDCEDSVAAV DADDKVVIYR NWLGLMKGDL 300
    AEEVSKGGST FTRTMNPDRV YTRADGSELT LHGRSLLFVR NVGHLMTNDA 350
    ILDKDGNEVP EGIQDGLFTS LIAIHDLNGN TSRKNSRTGS VYIVKPKMHG 400
    PEEAAFTNEL FGRVEDVLGL PRNTLKVGIM DEERRTTVNL KACIKAAKDR 450
    VVFINTGFLD RTGDEIHTSM EAGAVVRKGA MKSEKWIGAY ENNNVDVGLA 500
    TGLQGKAQIG KGMWAMPDLM AAMLEQKIGH PLAGANTAWV PSPTAATLHA 550
    LHYHKVDVFA RQAELAKRTP ASVDDILTIP LAPNTNWTAE EIKNEVDNNA 600
    QGILGYVVRW IDQGVGCSKV PDINDVGLME DRATLRISSQ LLANWLRHGV 650
    ISQEQVVESL KRMAVVVDRQ NASDPSYRPM APNFDDNVAF QAALELVVEG 700
    TRQPNGYTEP VLHRRRREFK AKNGL 725
    Length:725
    Mass (Da):78,660
    Last modified:March 1, 2001 - v1
    Checksum:i3669670A9E38D391
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG03871.1.
    PIRiH83586.
    RefSeqiNP_249173.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG03871; AAG03871; PA0482.
    GeneIDi879477.
    KEGGipae:PA0482.
    PATRICi19835216. VBIPseAer58763_0509.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG03871.1 .
    PIRi H83586.
    RefSeqi NP_249173.1. NC_002516.2.

    3D structure databases

    ProteinModelPortali Q9I636.
    SMRi Q9I636. Positions 2-722.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA0482.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG03871 ; AAG03871 ; PA0482 .
    GeneIDi 879477.
    KEGGi pae:PA0482.
    PATRICi 19835216. VBIPseAer58763_0509.

    Organism-specific databases

    PseudoCAPi PA0482.

    Phylogenomic databases

    eggNOGi COG2225.
    KOi K01638.
    OMAi SQFIENE.
    OrthoDBi EOG6HJ286.
    PhylomeDBi Q9I636.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

    Entry informationi

    Entry nameiMASZ_PSEAE
    AccessioniPrimary (citable) accession number: Q9I636
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3