Reviewed, UniProtKB/Swiss-Prot Q9I636 (MASZ_PSEAE)

Names and origin

Protein names

Recommended name:
    Malate synthase G
    EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	PA0482

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	725 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 725	725	Malate synthase G HAMAP MF_00641		PRO_0000166891
Sites
	Active site	340	1	Proton acceptor By similarity
	Active site	631	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I636-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 3669670A9E38D391	FASTA	725	78,660
	10 20 30 40 50 60 MTERVQVGGL QVAKVLFDFV NNEAIPGTGV SADTFWTGAE AVINDLAPKN KALLAKRDEL 70 80 90 100 110 120 QAKIDGWHQA RAGQAHDAVA YKAFLEEIGY LLPEAEDFQA GTQNVDDEIA RMAGPQLVVP 130 140 150 160 170 180 VMNARFALNA SNARWGSLYD ALYGTDVISE EGGAEKGKGY NKVRGDKVIA FARAFLDEAA 190 200 210 220 230 240 PLESGSHVDA TSYSVKNGAL VVALKNGSET GLKNAGQFLA FQGDAAKPQA VLLKHNGLHF 250 260 270 280 290 300 EIQIDPSSPV GQTDAAGVKD VLMEAALTTI MDCEDSVAAV DADDKVVIYR NWLGLMKGDL 310 320 330 340 350 360 AEEVSKGGST FTRTMNPDRV YTRADGSELT LHGRSLLFVR NVGHLMTNDA ILDKDGNEVP 370 380 390 400 410 420 EGIQDGLFTS LIAIHDLNGN TSRKNSRTGS VYIVKPKMHG PEEAAFTNEL FGRVEDVLGL 430 440 450 460 470 480 PRNTLKVGIM DEERRTTVNL KACIKAAKDR VVFINTGFLD RTGDEIHTSM EAGAVVRKGA 490 500 510 520 530 540 MKSEKWIGAY ENNNVDVGLA TGLQGKAQIG KGMWAMPDLM AAMLEQKIGH PLAGANTAWV 550 560 570 580 590 600 PSPTAATLHA LHYHKVDVFA RQAELAKRTP ASVDDILTIP LAPNTNWTAE EIKNEVDNNA 610 620 630 640 650 660 QGILGYVVRW IDQGVGCSKV PDINDVGLME DRATLRISSQ LLANWLRHGV ISQEQVVESL 670 680 690 700 710 720 KRMAVVVDRQ NASDPSYRPM APNFDDNVAF QAALELVVEG TRQPNGYTEP VLHRRRREFK AKNGL

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004091 Genomic DNA. Translation: AAG03871.1.
PIR	H83586.
RefSeq	NP_249173.1.
3D structure databases
HSSP	HSSP built from PDB template 1N8I based on UniProtKB Q50596.
ModBase	Search...
Genome annotation databases
GeneID	879477.
GenomeReviews	Gene locus PA0482 in contig AE004091_GR.
KEGG	pae:PA0482.
NMPDR	fig|208964.1.peg.483.
Organism-specific databases
PseudoCAP	PA0482.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9I636.
OMA	PKMHGPD.
Enzyme and pathway databases
BioCyc	PAER208964:PA0482-MON.
BRENDA	2.3.3.9. 354.
Family and domain databases
HAMAP	MF_00641. [Tree]
InterPro	IPR011076. Malate_synth-like_core. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_PSEAE

Accession

Primary (citable) accession number: Q9I636

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)