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Q9I636 (MASZ_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate synthase G

EC=2.3.3.9
Gene names
Name:glcB
Ordered Locus Names:PA0482
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length725 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA By similarity. HAMAP-Rule MF_00641

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP-Rule MF_00641

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00641

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP-Rule MF_00641

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00641

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00641.

Sequence similarities

Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentCytoplasm
   LigandMagnesium
Metal-binding
   Molecular functionTransferase
   PTMOxidation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmalate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 725725Malate synthase G HAMAP-Rule MF_00641
PRO_0000166891

Regions

Region125 – 1262Acetyl-CoA binding By similarity
Region457 – 4604Glyoxylate binding By similarity

Sites

Active site3401Proton acceptor By similarity
Active site6311Proton donor By similarity
Metal binding4321Magnesium By similarity
Metal binding4601Magnesium By similarity
Binding site1181Acetyl-CoA; via carbonyl oxygen By similarity
Binding site2761Acetyl-CoA By similarity
Binding site3131Acetyl-CoA By similarity
Binding site3401Glyoxylate By similarity
Binding site4321Glyoxylate By similarity
Binding site5411Acetyl-CoA; via carbonyl oxygen By similarity

Amino acid modifications

Modified residue6171Cysteine sulfenic acid (-SOH) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9I636 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 3669670A9E38D391

FASTA72578,660
        10         20         30         40         50         60 
MTERVQVGGL QVAKVLFDFV NNEAIPGTGV SADTFWTGAE AVINDLAPKN KALLAKRDEL 

        70         80         90        100        110        120 
QAKIDGWHQA RAGQAHDAVA YKAFLEEIGY LLPEAEDFQA GTQNVDDEIA RMAGPQLVVP 

       130        140        150        160        170        180 
VMNARFALNA SNARWGSLYD ALYGTDVISE EGGAEKGKGY NKVRGDKVIA FARAFLDEAA 

       190        200        210        220        230        240 
PLESGSHVDA TSYSVKNGAL VVALKNGSET GLKNAGQFLA FQGDAAKPQA VLLKHNGLHF 

       250        260        270        280        290        300 
EIQIDPSSPV GQTDAAGVKD VLMEAALTTI MDCEDSVAAV DADDKVVIYR NWLGLMKGDL 

       310        320        330        340        350        360 
AEEVSKGGST FTRTMNPDRV YTRADGSELT LHGRSLLFVR NVGHLMTNDA ILDKDGNEVP 

       370        380        390        400        410        420 
EGIQDGLFTS LIAIHDLNGN TSRKNSRTGS VYIVKPKMHG PEEAAFTNEL FGRVEDVLGL 

       430        440        450        460        470        480 
PRNTLKVGIM DEERRTTVNL KACIKAAKDR VVFINTGFLD RTGDEIHTSM EAGAVVRKGA 

       490        500        510        520        530        540 
MKSEKWIGAY ENNNVDVGLA TGLQGKAQIG KGMWAMPDLM AAMLEQKIGH PLAGANTAWV 

       550        560        570        580        590        600 
PSPTAATLHA LHYHKVDVFA RQAELAKRTP ASVDDILTIP LAPNTNWTAE EIKNEVDNNA 

       610        620        630        640        650        660 
QGILGYVVRW IDQGVGCSKV PDINDVGLME DRATLRISSQ LLANWLRHGV ISQEQVVESL 

       670        680        690        700        710        720 
KRMAVVVDRQ NASDPSYRPM APNFDDNVAF QAALELVVEG TRQPNGYTEP VLHRRRREFK 


AKNGL 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE004091 Genomic DNA. Translation: AAG03871.1.
PIRH83586.
RefSeqNP_249173.1. NC_002516.2.

3D structure databases

ProteinModelPortalQ9I636.
SMRQ9I636. Positions 2-722.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING208964.PA0482.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID879477.
KEGGpae:PA0482.
PATRIC19835216. VBIPseAer58763_0509.

Organism-specific databases

PseudoCAPPA0482.

Phylogenomic databases

eggNOGCOG2225.
KOK01638.
OMAPKMHGPD.
OrthoDBEOG6HJ286.
ProtClustDBPRK02999.

Enzyme and pathway databases

UniPathwayUPA00703; UER00720.

Family and domain databases

Gene3D2.170.170.11. 2 hits.
HAMAPMF_00641. Malate_synth_G.
InterProIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMSSF51645. SSF51645. 1 hit.
TIGRFAMsTIGR01345. malate_syn_G. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMASZ_PSEAE
AccessionPrimary (citable) accession number: Q9I636
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: March 1, 2001
Last modified: April 16, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways