Q9I618 (BIOB_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biotin synthase
EC=2.8.1.6

Gene names

Name:	bioB
Ordered Locus Names:	PA0500

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694
Catalytic activity	Dethiobiotin + sulfur + 2 S-adenosyl-L-methionine = biotin + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the radical SAM superfamily. Biotin synthase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW biotin synthase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Biotin synthase HAMAP-Rule MF_01694		PRO_0000381555
Sites
	Metal binding	59	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	63	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	66	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	103	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	134	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	194	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	266	1	Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I618 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: E46491B67DDF369A	FASTA	352	39,114
	10 20 30 40 50 60 MSATASVATR HDWSLAEVRA LFEQPFNDLL FQAQTVHRAH FDPNRVQVST LLSIKTGACP 70 80 90 100 110 120 EDCKYCPQSG HYNTGLDKEK LMEVQKVLEA AAEAKAIGST RFCMGAAWKH PSAKDMPYVL 130 140 150 160 170 180 EMVKGVKKLG LETCMTLGRL TQEQTQALAD AGLDYYNHNL DTSPEFYGNI ITTRTYSERL 190 200 210 220 230 240 QTLAYVREAG MKICSGGILG MGESVDDRAG LLIQLANLPE HPESVPINML VKVKGTPLAE 250 260 270 280 290 300 EKDVDPFDFI RTLAVARIMM PKSHVRLSAG REQMNEQMQA LAFMAGANSI FYGEKLLTTK 310 320 330 340 350 NPQAEKDMQL FARLGIKPEE REEHADEVHQ AAIEQALVEQ RESKLFYNAA SA

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG03889.1.
PIR	F83582.
RefSeq	NP_249191.1. NC_002516.2.
3D structure databases
HSSP	HSSP built from PDB template 1R30 based on UniProtKB P12996.
ProteinModelPortal	Q9I618.
SMR	Q9I618. Positions 10-320.
ModBase	Search...
Protein-protein interaction databases
STRING	208964.PA0500.
Protocols and materials databases
DNASU	877668.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	877668.
KEGG	pae:PA0500.
PATRIC	19835254. VBIPseAer58763_0528.
Organism-specific databases
PseudoCAP	PA0500.
Phylogenomic databases
eggNOG	COG0502.
KO	K01012.
OMA	RIMMPAS.
ProtClustDB	CLSK2299172.
Enzyme and pathway databases
UniPathway	UPA00078; UER00162.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01694. BioB.
InterPro	IPR013785. Aldolase_TIM. IPR010722. Biotin/thiamin_synth-assoc. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM. [Graphical view]
Pfam	PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF001619. Biotin_synth. 1 hit.
SMART	SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00433. bioB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOB_PSEAE

Accession

Primary (citable) accession number: Q9I618

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program