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Q9I617 (BIOF_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
8-amino-7-oxononanoate synthase
Short name=AONS
EC=2.3.1.47
Alternative name(s):
7-keto-8-amino-pelargonic acid synthase
Short name=7-KAP synthase
Short name=KAPA synthase
8-amino-7-ketopelargonate synthase
L-alanine--pimeloyl-CoA ligase
Gene names
Name:bioF
Ordered Locus Names:PA0501
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length401 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the decarboxylative condensation of pimeloyl-CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide By similarity. HAMAP MF_01693

Catalytic activity

6-carboxyhexanoyl-CoA + L-alanine = 8-amino-7-oxononanoate + CoA + CO2. HAMAP MF_01693

Cofactor

Pyridoxal phosphate By similarity. HAMAP MF_01693

Pathway

Cofactor biosynthesis; biotin biosynthesis; 8-amino-7-oxononanoate from pimeloyl-CoA: step 1/1. HAMAP MF_01693

Subunit structure

Homodimer By similarity. HAMAP MF_01693

Sequence similarities

Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4014018-amino-7-oxononanoate synthase HAMAP MF_01693
PRO_0000287825

Regions

Region106 – 1072Pyridoxal phosphate binding By similarity
Region201 – 2044Pyridoxal phosphate binding By similarity
Region233 – 2364Pyridoxal phosphate binding By similarity

Sites

Binding site191Substrate By similarity
Binding site1311Substrate By similarity
Binding site1761Pyridoxal phosphate By similarity
Binding site3501Substrate By similarity

Amino acid modifications

Modified residue2361N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9I617 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: BA92F08B47BD0163

FASTA40142,237
        10         20         30         40         50         60 
MSFDLASRLA SRRAEDLYRQ RPLLESAQGP DVVVDGQPLL AFCSNDYLGL ANHPEVIAAL 

        70         80         90        100        110        120 
RAGAERWGVG GGASHLVVGH SGPHHELELA LAEFTGRPRA LLFSTGYMAN LGAVAALVGK 

       130        140        150        160        170        180 
GDTVLEDRLN HASLLDAGLL SGARFSRYLH NDPASLAARL DKAEGNTLVV TDGVFSMDGN 

       190        200        210        220        230        240 
LADLPALAAV AQARGAWLMV DDAHGFGPLG ASGGGIVEHF GLGQEQVPVL IGTLGKGFGT 

       250        260        270        280        290        300 
AGAFVAGSEE LIETLIQYAR PYIYTTSQPP AVACATLKSL ELLRRESWRR QHLAALIARF 

       310        320        330        340        350        360 
RHGAEALGLT LMDSFTPIQP ILVGGSRQAV ALAGMLRARG IMVGAIRPPT VPANSARLRV 

       370        380        390        400 
TLSAAHSEAQ VDRLLEALGE SWRQLSSSLL AEIEAEEGDD A

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE004091 Genomic DNA. Translation: AAG03890.1.
PIRG83582.
RefSeqNP_249192.1. NC_002516.2.

3D structure databases

HSSPHSSP built from PDB template 1BS0 based on UniProtKB P12998.
ProteinModelPortalQ9I617.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID879619.
GenomeReviewsGene locus PA0501 in contig AE004091_GR.
KEGGpae:PA0501.
NMPDRfig|208964.1.peg.502.
PATRIC19835256. VBIPseAer58763_0529.

Organism-specific databases

PseudoCAPPA0501.

Phylogenomic databases

HOGENOMHBG649839.
OMAATVMAMS.
ProtClustDBPRK05958.

Enzyme and pathway databases

BioCycPAER208964:PA0501-MONOMER.

Family and domain databases

HAMAPMF_01693. BioF_aminotrans_2.
[Tree]
InterProIPR022834. Amino_oxononanoate_synth.
IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR004723. BioF.
IPR015424. PyrdxlP-dep_Trfase_major_dom.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
Gene3DG3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KOK00652.
PfamPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMsTIGR00858. BioF. 1 hit.
PROSITEPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBIOF_PSEAE
AccessionPrimary (citable) accession number: Q9I617
Entry history
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: March 1, 2001
Last modified: January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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