Q9I614 (BIOD_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	PA0504

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	228 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 228	228	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_0000187982
Regions
	Nucleotide binding	12 – 17	6	ATP By similarity
	Nucleotide binding	116 – 119	4	ATP By similarity
	Nucleotide binding	205 – 207	3	ATP By similarity
Sites
	Metal binding	12	1	Magnesium 1 By similarity
	Metal binding	16	1	Magnesium 2 By similarity
	Metal binding	54	1	Magnesium 2 By similarity
	Metal binding	116	1	Magnesium 2 By similarity
	Binding site	41	1	Substrate By similarity
	Binding site	54	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I614 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 4CC964E353B3085A	FASTA	228	23,337
	10 20 30 40 50 60 MPAFFVTGTD TEIGKTTIAA GLLHAARSAG LSTAAAKPVA SGCEPTAQGL RNGDALVLLG 70 80 90 100 110 120 QCSLALAYEQ VNPLAFAPAI APHLAAREAG VELSAARLHE AVREVLALQA DFTLVEGAGG 130 140 150 160 170 180 WRVPLLGREN LSDLARLLAL PVVLVVGVRL GCINHALLSA EAILGDGLAL AGWVANVVDP 190 200 210 220 ATSRLEENLA TLAERLPAPC LGRVPRLEEA TPAAVAAHLD LRPLGIGL

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG03893.1.
PIR	B83583.
RefSeq	NP_249195.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9I614.
ModBase	Search...
2D gel databases
REPRODUCTION-2DPAGE	Q9I614.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	878220.
GenomeReviews	Gene locus PA0504 in contig AE004091_GR.
KEGG	pae:PA0504.
PATRIC	19835262. VBIPseAer58763_0532.
Organism-specific databases
PseudoCAP	PA0504.
Phylogenomic databases
HOGENOM	HBG650065.
OMA	CINHAIL.
ProtClustDB	PRK00090.
Enzyme and pathway databases
BioCyc	PAER208964:PA0504-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR002586. CbiA_P_synth. IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_PSEAE

Accession

Primary (citable) accession number: Q9I614

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program