Q9I5U8 (GLPE_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiosulfate sulfurtransferase glpE
EC=2.8.1.1

Gene names

Name:	glpE
Ordered Locus Names:	PA0589

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	110 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes, although with low efficiency, the sulfur transfer reaction from thiosulfate to cyanide By similarity. HAMAP MF_01009
Catalytic activity	Thiosulfate + cyanide = sulfite + thiocyanate. HAMAP MF_01009
Subcellular location	Cytoplasm By similarity HAMAP MF_01009.
Sequence similarities	Belongs to the glpE family. Contains 1 rhodanese domain.

Ontologies

Keywords
Biological process	Glycerol metabolism
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiosulfate sulfurtransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 110	110	Thiosulfate sulfurtransferase glpE HAMAP MF_01009		PRO_0000200559
Regions
	Domain	17 – 105	89	Rhodanese
Sites
	Active site	65	1	Cysteine persulfide intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I5U8 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: B0C009A0FDDFD1FC	FASTA	110	11,963
	10 20 30 40 50 60 MSDTFQRIAP EQVRQLRENG AQVVDIRDPQ SFAVGHISGS RHIDNHSVAD FIAAADLDAP 70 80 90 100 110 LVVVCYHGNS SQSAAAYFIQ QGFSDVYSLD GGFELWRSVY PADTSSGEAE

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG03978.1.
PIR	E83571.
RefSeq	NP_249280.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9I5U8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	880468.
GenomeReviews	Gene locus PA0589 in contig AE004091_GR.
KEGG	pae:PA0589.
NMPDR	fig|208964.1.peg.590.
PATRIC	19835450. VBIPseAer58763_0625.
Organism-specific databases
PseudoCAP	PA0589.
Phylogenomic databases
HOGENOM	HBG743107.
OMA	AWHRHFP.
ProtClustDB	PRK00162.
Enzyme and pathway databases
BioCyc	PAER208964:PA0589-MONOMER.
Family and domain databases
HAMAP	MF_01009. Thiosulf_sulfurtr. [Tree]
InterPro	IPR001763. Rhodanese-like. IPR023695. Thiosulf_sulfurTrfase. [Graphical view]
Gene3D	G3DSA:3.40.250.10. Rhodanese-like. 1 hit.
KO	K02439.
Pfam	PF00581. Rhodanese. 1 hit. [Graphical view]
SMART	SM00450. RHOD. 1 hit. [Graphical view]
SUPFAM	SSF52821. Rhodanese-like. 1 hit.
PROSITE	PS50206. RHODANESE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPE_PSEAE

Accession

Primary (citable) accession number: Q9I5U8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2002
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program