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Q9I5U4

- PDXA1_PSEAE

UniProt

Q9I5U4 - PDXA1_PSEAE

Protein

4-hydroxythreonine-4-phosphate dehydrogenase 1

Gene

pdxA1

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).By similarity

    Catalytic activityi

    4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.

    Cofactori

    Binds 1 divalent metal cation per subunit. Can probably use ions such as zinc, magnesium or cobalt.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei135 – 1351SubstrateBy similarity
    Binding sitei136 – 1361SubstrateBy similarity
    Metal bindingi165 – 1651Divalent metal cation; shared with dimeric partner
    Metal bindingi210 – 2101Divalent metal cation; shared with dimeric partner
    Metal bindingi265 – 2651Divalent metal cation; shared with dimeric partner
    Binding sitei273 – 2731SubstrateBy similarity
    Binding sitei282 – 2821SubstrateBy similarity
    Binding sitei291 – 2911SubstrateBy similarity

    GO - Molecular functioni

    1. 4-hydroxythreonine-4-phosphate dehydrogenase activity Source: UniProtKB-HAMAP
    2. cobalt ion binding Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. NAD binding Source: InterPro
    5. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. pyridoxal phosphate biosynthetic process Source: UniProtKB-HAMAP
    2. pyridoxine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Pyridoxine biosynthesis

    Keywords - Ligandi

    Cobalt, Magnesium, Metal-binding, NAD, NADP, Zinc

    Enzyme and pathway databases

    UniPathwayiUPA00244; UER00312.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxythreonine-4-phosphate dehydrogenase 1 (EC:1.1.1.262)
    Alternative name(s):
    4-(phosphohydroxy)-L-threonine dehydrogenase 1
    Gene namesi
    Name:pdxA1
    Ordered Locus Names:PA0593
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA0593.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3283284-hydroxythreonine-4-phosphate dehydrogenase 1PRO_0000188815Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi208964.PA0593.

    Structurei

    Secondary structure

    1
    328
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi5 – 84
    Helixi16 – 238
    Beta strandi32 – 365
    Helixi38 – 4811
    Beta strandi53 – 564
    Beta strandi71 – 766
    Helixi90 – 923
    Helixi93 – 10816
    Beta strandi113 – 1175
    Helixi122 – 1276
    Helixi135 – 1428
    Beta strandi149 – 1546
    Beta strandi157 – 1637
    Helixi168 – 1703
    Helixi171 – 1744
    Helixi177 – 19317
    Beta strandi201 – 2055
    Helixi209 – 2124
    Turni213 – 2175
    Helixi220 – 2234
    Helixi225 – 2339
    Turni234 – 2363
    Beta strandi238 – 2436
    Helixi245 – 2484
    Helixi251 – 2544
    Beta strandi258 – 2647
    Helixi265 – 27612
    Beta strandi281 – 2899
    Beta strandi291 – 2944
    Helixi300 – 3023
    Turni303 – 3053
    Helixi311 – 32616

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1YXOX-ray2.01A/B1-328[»]
    ProteinModelPortaliQ9I5U4.
    SMRiQ9I5U4. Positions 1-328.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9I5U4.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PdxA family.Curated

    Phylogenomic databases

    eggNOGiCOG1995.
    KOiK00097.
    OMAiWPERPAK.
    OrthoDBiEOG6GN6ZC.
    PhylomeDBiQ9I5U4.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    HAMAPiMF_00536. PdxA.
    InterProiIPR024084. IsoPropMal-DH-like_dom.
    IPR005255. PdxA.
    [Graphical view]
    PfamiPF04166. PdxA. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00557. pdxA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9I5U4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLRFALTPG EPAGIGPDLC LLLARSAQPH PLIAIASRTL LQERAGQLGL    50
    AIDLKDVSPA AWPERPAKAG QLYVWDTPLA APVRPGQLDR ANAAYVLETL 100
    TRAGQGCLDG HFAGMITAPV HKGVINEAGI PFSGHTEFLA DLTHTAQVVM 150
    MLATRGLRVA LATTHLPLRE VADAISDERL TRVARILHAD LRDKFGIAHP 200
    RILVCGLNPH AGEGGHLGRE EIEVIEPCLE RLRGEGLDLI GPLPADTLFT 250
    PKHLEHCDAV LAMYHDQGLP VLKYKGFGAA VNVTLGLPII RTSVDHGTAL 300
    DLAGSGRIDS GSLQVALETA YQMAASRC 328
    Length:328
    Mass (Da):34,923
    Last modified:March 1, 2001 - v1
    Checksum:i2C666738F21D7B8C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG03982.1.
    PIRiA83572.
    RefSeqiNP_249284.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG03982; AAG03982; PA0593.
    GeneIDi880733.
    KEGGipae:PA0593.
    PATRICi19835458. VBIPseAer58763_0629.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG03982.1 .
    PIRi A83572.
    RefSeqi NP_249284.1. NC_002516.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1YXO X-ray 2.01 A/B 1-328 [» ]
    ProteinModelPortali Q9I5U4.
    SMRi Q9I5U4. Positions 1-328.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA0593.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG03982 ; AAG03982 ; PA0593 .
    GeneIDi 880733.
    KEGGi pae:PA0593.
    PATRICi 19835458. VBIPseAer58763_0629.

    Organism-specific databases

    PseudoCAPi PA0593.

    Phylogenomic databases

    eggNOGi COG1995.
    KOi K00097.
    OMAi WPERPAK.
    OrthoDBi EOG6GN6ZC.
    PhylomeDBi Q9I5U4.

    Enzyme and pathway databases

    UniPathwayi UPA00244 ; UER00312 .

    Miscellaneous databases

    EvolutionaryTracei Q9I5U4.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    HAMAPi MF_00536. PdxA.
    InterProi IPR024084. IsoPropMal-DH-like_dom.
    IPR005255. PdxA.
    [Graphical view ]
    Pfami PF04166. PdxA. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00557. pdxA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
    2. "Crystal structure of pyridoxal phosphate biosynthetic protein pdxA PA0593."
      Midwest center for structural genomics (MCSG)
      Submitted (APR-2005) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.01 ANGSTROMS) IN COMPLEX WITH MAGNESIUM IONS, COFACTOR, SUBUNIT.

    Entry informationi

    Entry nameiPDXA1_PSEAE
    AccessioniPrimary (citable) accession number: Q9I5U4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 31, 2002
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The active site is located at the dimer interface.By similarity

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3