Reviewed, UniProtKB/Swiss-Prot Q9I5Q3 (SYY2_PSEAE)

Names and origin

Protein names

Recommended name:
    Tyrosyl-tRNA synthetase 2
    EC=6.1.1.1
Alternative name(s):
    Tyrosine--tRNA ligase 2
      Short name=TyrRS 2

Gene names

Name:	tyrS2
Ordered Locus Names:	PA0668

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02007
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 2 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 399	399	Tyrosyl-tRNA synthetase 2 HAMAP MF_02007		PRO_0000236750
Regions
	Domain	336 – 396	61	S4 RNA-binding
	Motif	42 – 51	10	"HIGH" region HAMAP MF_02007
	Motif	226 – 230	5	"KMSKS" region HAMAP MF_02007
Sites
	Binding site	229	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I5Q3-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 14CF0EB768EE833D	FASTA	399	44,130
	10 20 30 40 50 60 MKSVEEQLAL IQRGADEILV EAELVAKLKR GQPLRIKAGF DPTAPDLHLG HTVLINKLRQ 70 80 90 100 110 120 FQDLGHQVIF LIGDFTGMIG DPSGKSVTRP PLTREQVLEN AETYKSQVFK ILDPAKTEVA 130 140 150 160 170 180 FNSTWMDQLT PADFIRLASQ YTVARMLERD DFSKRYASNQ PIAIHEFLYP LVQGYDSVAL 190 200 210 220 230 240 KADVELGGTD QKFNLLMGRE LQRAYGQEAQ VILTMPLLEG LDGVKKMSKS LGNYIGIQEA 250 260 270 280 290 300 PGVMYSKLVS IPDTLMWRYF ELLSFRSLDE IDSFRKDVEA GANPRDIKIK LAEEIVARFH 310 320 330 340 350 360 GEEAAASAHK SAGNRLKEGE LPEDLPEIEL SSPEDMPVAS VLNKAGLVKN AAAARDLLGA 370 380 390 GSVKVDGQVV DRTFMLALGE TRVFQAGKKA FARITLKAE

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004091 Genomic DNA. Translation: AAG04057.1.
PIR	C83563.
RefSeq	NP_249359.1.
3D structure databases
HSSP	HSSP built from PDB template 1H3F based on UniProtKB P83453.
ModBase	Search...
Genome annotation databases
GeneID	880817.
GenomeReviews	Gene locus PA0668 in contig AE004091_GR.
KEGG	pae:PA0668.
Organism-specific databases
PseudoCAP	PA0668.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9I5Q3.
OMA	YVVQVGK.
Enzyme and pathway databases
BioCyc	PAER208964:PA0668-MON.
BRENDA	6.1.1.1. 354.
Family and domain databases
HAMAP	MF_02007. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ib. IPR014729. Rossmann-like_a/b/a_fold. IPR002942. S4_RNA_bd. IPR002307. Tyr-tRNA-synth_Ib_bac/mito. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF01479. S4. 1 hit. PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
SMART	SM00363. S4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY2_PSEAE

Accession

Primary (citable) accession number: Q9I5Q3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)