Reviewed, UniProtKB/Swiss-Prot Q9I5Q2 (DNAE2_PSEAE)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	PA0669

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	1031 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW error-prone DNA repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1031	1031	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103388

Sequences

	Sequence		Length	Mass (Da)
	Q9I5Q2-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 06C6EB22D9F4AD40	FASTA	1,031	115,769
	10 20 30 40 50 60 MAAWLVRMSA YAELHCLSNF SFQRGASSAT ELFARAARLG YRALAITDEC SLAGIVRAWQ 70 80 90 100 110 120 AAREHQVQLI VGSEIRLEQG PKLVLLAEDL EGYQNLCRLI TRGRRQADKG HYRLLREDLQ 130 140 150 160 170 180 QPLAGLLAIW LPNDHGDEQA AWLRERFPQR LWLGVELHRG ADDDARLDKL LALASHLRLP 190 200 210 220 230 240 PVACGDVHMH ARGRRALQDC MTAIRNHLPV SEAGAYLFPN GERHLRTLEA LQGIYPQALL 250 260 270 280 290 300 AETLKIAERC RFDLEQLKYQ YPRELVPAGH DPASWLRHLT EQGIARRWPN GASAKVRKQI 310 320 330 340 350 360 ERELELIAEL GYESYFLTVQ DIVAFARGRK ILCQGRGSAA NSAVCFALGI TELDPDRTNL 370 380 390 400 410 420 LFERFLSRER NEPPDIDVDF EHERREEVIQ YVFTRYGRQR AALTAVVSTY HGAGAVRDVA 430 440 450 460 470 480 KALGLPPEQV DVLANCCGRW SDQAPSAERL EEAGFDPQSP ILRRVLALTD ELIGFPRHLS 490 500 510 520 530 540 QHPGGFVISE QPLDTLVPVE NASMAERTVI QWDKDDLDAV GLLKVDVLAL GMLSALRRSF 550 560 570 580 590 600 DLIHALRGGK RLSIASIPSE DPATYEMISR ADTIGVFQIE SRAQMAMLPR LRPQKFYDLV 610 620 630 640 650 660 IQVAIVRPGP IQGDMVHPYL RRRNGEEPVA YPSAELEEVF ERTLGVPLFQ EQVMELAIVA 670 680 690 700 710 720 ADYTPGEADE LRRSMAAWKR HGGLEHHRER LTRGMLAKGY EADFAARIFE QIKGFGSYGF 730 740 750 760 770 780 PESHAASFAL LTYASSWLKR HEPAAFACAL INSWPMGFYS PDQLLQDARR HALQTRPVDV 790 800 810 820 830 840 RHSGWDCSLE SFGQAQPAIR LGLRMIRGFR EEDARRIEQV REAEPFLDVH DLGRRARLDA 850 860 870 880 890 900 RALELLADAG ALRGLAGHRH KARWAVASVE PQLPLFAEGT AIEESTVSLP LPSRGEELLS 910 920 930 940 950 960 DYALLGTTLG PHPLKLLRGQ LKARRCRDSR ELAKLGHGRP IRVAGLVVGR QRPQTASGIT 970 980 990 1000 1010 1020 FITLEDEFGM VNVVVRHDLA ERQRRPFLES RLLQVEGILE SSGEVRHVIA GRLHDLTPLL 1030 TGLDVRSRDF H

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004091 Genomic DNA. Translation: AAG04058.1.
PIR	F83561.
RefSeq	NP_249360.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	880686.
GenomeReviews	Gene locus PA0669 in contig AE004091_GR.
KEGG	pae:PA0669.
NMPDR	fig|208964.1.peg.670.
Organism-specific databases
PseudoCAP	PA0669.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9I5Q2.
OMA	Q9I5Q2. MGFYSPD.
Enzyme and pathway databases
BioCyc	PAER208964:PA0669-MON.
BRENDA	2.7.7.7. 354.
Family and domain databases
HAMAP	MF_01902. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. PHP_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_PSEAE

Accession

Primary (citable) accession number: Q9I5Q2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)