Probable 3-hydroxyisobutyrate dehydrogenase - Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Preferred order of sections

Names and origin

Protein names

Submitted name:
Probable 3-hydroxyisobutyrate dehydrogenase EMBL AAG04132.1

Gene names

Ordered Locus Names:

PA0743

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Ligand	NAD PDB 3Q3C Nucleotide-binding PDB 3Q3C
Technical term	3D-structure PDB 3OBB PDB 3Q3C Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pentose-phosphate shunt Inferred from electronic annotation. Source: InterPro valine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	3-hydroxyisobutyrate dehydrogenase activity Inferred from electronic annotation. Source: InterPro NAD binding Inferred from electronic annotation. Source: InterPro phosphogluconate dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Regions

Nucleotide binding

11 – 12

2

NAD PDB 3Q3C

Sites

Binding site

31

1

NAD PDB 3Q3C

Binding site

66

1

NAD; via carbonyl oxygen PDB 3Q3C

Binding site

96

1

NAD; via amide nitrogen PDB 3Q3C

Binding site

246

1

NAD PDB 3Q3C

Sequences

Sequence

Length

Mass (Da)

Tools

Q9I5I6 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 27100627BEBB469C
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FASTA

298

30,754

        10         20         30         40         50         60 
MKQIAFIGLG HMGAPMATNL LKAGYLLNVF DLVQSAVDGL VAAGASAARS ARDAVQGADV 

        70         80         90        100        110        120 
VISMLPASQH VEGLYLDDDG LLAHIAPGTL VLECSTIAPT SARKIHAAAR ERGLAMLDAP 

       130        140        150        160        170        180 
VSGGTAGAAA GTLTFMVGGD AEALEKARPL FEAMGRNIFH AGPDGAGQVA KVCNNQLLAV 

       190        200        210        220        230        240 
LMIGTAEAMA LGVANGLEAK VLAEIMRRSS GGNWALEVYN PWPGVMENAP ASRDYSGGFM 

       250        260        270        280        290 
AQLMAKDLGL AQEAAQASAS STPMGSLALS LYRLLLKQGY AERDFSVVQK LFDPTQGQ

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.Q., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Structure and activity of the L-serine dehydrogenase PA0743 from Pseudomonas aeruginosa." Tchigvintsev A., Singer A., Tan K., Brown G., Flick R., Evdokimonva E., Joachimiak A., Savchenko A., Yakunin A.F. Submitted (AUG-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS).
[3]	"Structure and activity of the L-serine dehydrogenase pa0743 from Pseudomonas aeruginosa." Tchigvintsev A., Singer A.U., Tan K., Brown G., Flick R., Edwards A.M., Savchenko A., Yakunin A.F. Submitted (DEC-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) IN COMPLEX WITH NAD.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE004091 Genomic DNA. Translation: AAG04132.1.

PIR

B83553.

RefSeq

NP_249434.1. NC_002516.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3OBB	X-ray	2.20	A	1-298	[»]
3Q3C	X-ray	2.30	A	1-298	[»]

ProteinModelPortal

Q9I5I6.

SMR

Q9I5I6. Positions 1-296.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

882135.

GenomeReviews

Gene locus PA0743 in contig AE004091_GR.

KEGG

pae:PA0743.

NMPDR

fig|208964.1.peg.744.

PATRIC

19835758. VBIPseAer58763_0772.

Organism-specific databases

PseudoCAP

PA0743.

Phylogenomic databases

HOGENOM

HBG729179.

OMA

NVPSSKG.

ProtClustDB

CLSK866084.

Enzyme and pathway databases

BioCyc

PAER208964:PA0743-MONOMER.

Family and domain databases

InterPro

IPR002204. 3-OH-isobutyrate_DH-rel_CS.
IPR015815. 3hydroxyacid_DH/Rdtase.
IPR008927. 6-PGluconate_DH_C-like.
IPR006115. 6PGDH_NADP-bd.
IPR013328. DH_multihelical.
IPR011548. IsoBut3OH_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
G3DSA:1.10.1040.10. Opine_DH. 1 hit.

KO

K00020.

PANTHER

PTHR22981. 3hydroxy_acid_DH. 1 hit.

Pfam

PF03446. NAD_binding_2. 1 hit.
[Graphical view]

SUPFAM

SSF48179. 6DGDH_C_like. 1 hit.

TIGRFAMs

TIGR01692. HIBADH. 1 hit.

PROSITE

PS00895. 3_HYDROXYISOBUT_DH. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q9I5I6_PSEAE

Accession

Primary (citable) accession number: Q9I5I6

Entry history

Integrated into UniProtKB/TrEMBL:	March 1, 2001
Last sequence update:	March 1, 2001
Last modified:	December 14, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)