Q9I5G7 (LEP_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal peptidase I
Short name=SPase I
EC=3.4.21.89

Alternative name(s):
Leader peptidase I

Gene names

Name:	lepB
Ordered Locus Names:	PA0768

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Signal peptidase I		PRO_0000109513
Regions
	Transmembrane	4 – 22	19	Helical; Potential
	Topological domain	23 – 58	36	Cytoplasmic Potential
	Transmembrane	59 – 77	19	Helical; Potential
	Topological domain	78 – 284	207	Periplasmic Potential
Sites
	Active site	90	1	By similarity
	Active site	145	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I5G7 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: ACA143F48F90ED2C	FASTA	284	32,103
	10 20 30 40 50 60 MTLNFPLLLV IAVAVCGALA LVDLVLFAPR RRAAISSYEG QVNEPDPAVL EKLNKEPLLV 70 80 90 100 110 120 EYGKSFFPVL FIVLVLRSFL VEPFQIPSGS MKPTLEVGDF ILVNKFAYGI RLPVLDTKVI 130 140 150 160 170 180 PIGDPQRGDV MVFRYPSEPN INYIKRVVGL PGDTVRYTKE KRLYVNGELV AEKLVGEEPG 190 200 210 220 230 240 TLGSVTLYQE KLGQAEHLIR KEMSRYRIEP DRQWTIPAGH YFMMGDNRDN SNDSRYWNDP 250 260 270 280 KIPKDLLGMV PDRNIVGKAF AVWMSWPDPK MSNLPNFSRV GVIH

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG04157.1.
PIR	H83550.
RefSeq	NP_249459.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9I5G7.
ModBase	Search...
Protein family/group databases
MEROPS	S26.001.
Proteomic databases
PRIDE	Q9I5G7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	882104.
GenomeReviews	Gene locus PA0768 in contig AE004091_GR.
KEGG	pae:PA0768.
PATRIC	19835810. VBIPseAer58763_0798.
Organism-specific databases
PseudoCAP	PA0768.
Phylogenomic databases
HOGENOM	HBG596607.
OMA	RYPSDPN.
ProtClustDB	CLSK866103.
Enzyme and pathway databases
BioCyc	PAER208964:PA0768-MONOMER.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019757. Pept_S26A_signal_pept_1_Lys-AS. IPR019756. Pept_S26A_signal_pept_1_Ser-AS. IPR019759. Peptidase_S24_S26. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 2 hits.
KO	K03100.
Pfam	PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
SUPFAM	SSF51306. Pept_S24_S26_C. 1 hit.
TIGRFAMs	TIGR02227. Sigpep_I_bact. 1 hit.
PROSITE	PS00501. SPASE_I_1. 1 hit. PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP_PSEAE

Accession

Primary (citable) accession number: Q9I5G7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program