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Q9I5A5 (PTA_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphate acetyltransferase

EC=2.3.1.8
Alternative name(s):
Phosphotransacetylase
Gene names
Name:pta
Ordered Locus Names:PA0835
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length704 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in acetate metabolism. In combination with LdhA and AckA, allows fermentation of pyruvate, enhancing long-term survival under anaerobic conditions. Ref.2

Catalytic activity

Acetyl-CoA + phosphate = CoA + acetyl phosphate. Ref.2

Enzyme regulation

Activity is increased under anaerobic growth conditions. Ref.2

Pathway

Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 2/2.

Subunit structure

Homohexamer By similarity.

Subcellular location

Cytoplasm Potential.

Induction

The ackA-pta operon is induced under oxygen-limiting conditions by the oxygen regulator Anr and DNA-binding integration host factor. Ref.2

Domain

The N-terminal region seems to be important for proper quaternary structure. The C-terminal region contains the substrate-binding site By similarity.

Disruption phenotype

Decreased survival under anaerobic conditions. Ref.2

Sequence similarities

In the N-terminal section; belongs to the CobB/CobQ family.

In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   Molecular functionAcyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processacetyl-CoA biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionphosphate acetyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 704704Phosphate acetyltransferase
PRO_0000405549

Regions

Region379 – 704326Phosphate acetyltransferase

Sequences

Sequence LengthMass (Da)Tools
Q9I5A5 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: EEEF0FFDB388A88C

FASTA70475,697
        10         20         30         40         50         60 
MHTFFIAPTG FGVGLTSISL GLLRALERAG LKVGFFKPIA QLHPGDLGPE RSSELVARTH 

        70         80         90        100        110        120 
GLDTPKPLPL AQVERMLGDG QLDELLEEII SLYQRAAADK DVVIVEGMVP TRHASYAARV 

       130        140        150        160        170        180 
NFHLAKSLDA EVILVSAPEN ETLTELTDRI EIQAQLFGGP RDPKVLGVIL NKVRGEADAA 

       190        200        210        220        230        240 
NAEDGVADFA RRLTEHSPLL RDDFRLIGCI PWQDELNAAR TRDIADLLSA RVINAGDYEQ 

       250        260        270        280        290        300 
RRVQKIVLCA RAVPNTVQLL KPGVLVVTPG DRDDIILAAS LAAMNGVPLA GLLLCSDFPP 

       310        320        330        340        350        360 
DPRIMELCRG ALQGGLPVLS VATGSYDTAT NLNRMNKEIP VDDRERAERV TEFVAGHIDF 

       370        380        390        400        410        420 
EWLKQRCGTP RELRLSPPAF RYQVVQRAQK AGKRIVLPEG SEPRTVQAAA ICQARGIARC 

       430        440        450        460        470        480 
VLLAKPEEVQ AVAQAQGIVL PEGLEIIDPD LVRQRYVEPM VELRKGKGLN APMAEQQLED 

       490        500        510        520        530        540 
SVVLATMMLA LDEVDGLVSG AIHTTASTIR PALQLIKTAP GYNLVSSVFF MLLPDQVLVY 

       550        560        570        580        590        600 
GDCAVNPDPS ASDLAEIAVQ SAASAQAFGI PARVAMISYS TGDSGSGVDV DKVREATRLA 

       610        620        630        640        650        660 
REQRPDLLID GPLQYDAAAI ASVGRQKAPN SPVAGQATVF IFPDLNTGNT TYKAVQRSAD 

       670        680        690        700 
CVSVGPMLQG LRKPVNDLSR GALVEDIVYT IALTAIQADA QAPA 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen."
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. expand/collapse author list , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.
Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]"Long-term anaerobic survival of the opportunistic pathogen Pseudomonas aeruginosa via pyruvate fermentation."
Eschbach M., Schreiber K., Trunk K., Buer J., Jahn D., Schobert M.
J. Bacteriol. 186:4596-4604(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, INDUCTION, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE004091 Genomic DNA. Translation: AAG04224.1.
PIRC83541.
RefSeqNP_249526.1. NC_002516.2.

3D structure databases

ProteinModelPortalQ9I5A5.
SMRQ9I5A5. Positions 394-698.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING208964.PA0835.

Proteomic databases

PRIDEQ9I5A5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG04224; AAG04224; PA0835.
GeneID882123.
KEGGpae:PA0835.
PATRIC19835948. VBIPseAer58763_0866.

Organism-specific databases

PseudoCAPPA0835.

Phylogenomic databases

eggNOGCOG0280.
KOK13788.
OMAKPIAQPH.
OrthoDBEOG6BKJ5W.
PhylomeDBQ9I5A5.

Enzyme and pathway databases

UniPathwayUPA00340; UER00459.

Family and domain databases

Gene3D3.40.1390.20. 1 hit.
3.40.50.300. 1 hit.
InterProIPR010766. DRTGG.
IPR016475. P-Actrans_bac.
IPR027417. P-loop_NTPase.
IPR004614. P_AcTrfase.
IPR002505. PTA_PTB.
IPR028979. Ser_kin/Pase_Hpr_N_like.
[Graphical view]
PfamPF07085. DRTGG. 1 hit.
PF01515. PTA_PTB. 1 hit.
[Graphical view]
PIRSFPIRSF006107. PhpActrans_proteobac. 1 hit.
SUPFAMSSF52540. SSF52540. 1 hit.
SSF75138. SSF75138. 1 hit.
TIGRFAMsTIGR00651. pta. 1 hit.
ProtoNetSearch...

Entry information

Entry namePTA_PSEAE
AccessionPrimary (citable) accession number: Q9I5A5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways