Q9I5A5 (PTA_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphate acetyltransferase
EC=2.3.1.8

Alternative name(s):
Phosphotransacetylase

Gene names

Name:	pta
Ordered Locus Names:	PA0835

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	704 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in acetate metabolism. In combination with LdhA and AckA, allows fermentation of pyruvate, enhancing long-term survival under anaerobic conditions. Ref.2
Catalytic activity	Acetyl-CoA + phosphate = CoA + acetyl phosphate. Ref.2
Enzyme regulation	Activity is increased under anaerobic growth conditions. Ref.2
Pathway	Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 2/2.
Subunit structure	Homohexamer By similarity.
Subcellular location	Cytoplasm Potential.
Induction	The ackA-pta operon is induced under oxygen-limiting conditions by the oxygen regulator Anr and DNA-binding integration host factor. Ref.2
Domain	The N-terminal region seems to be important for proper quaternary structure. The C-terminal region contains the substrate-binding site By similarity.
Disruption phenotype	Decreased survival under anaerobic conditions. Ref.2
Sequence similarities	In the N-terminal section; belongs to the CobB/CobQ family. In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphate acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 704	704	Phosphate acetyltransferase		PRO_0000405549
Regions
	Region	379 – 704	326	Phosphate acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	Q9I5A5 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: EEEF0FFDB388A88C	FASTA	704	75,697
	10 20 30 40 50 60 MHTFFIAPTG FGVGLTSISL GLLRALERAG LKVGFFKPIA QLHPGDLGPE RSSELVARTH 70 80 90 100 110 120 GLDTPKPLPL AQVERMLGDG QLDELLEEII SLYQRAAADK DVVIVEGMVP TRHASYAARV 130 140 150 160 170 180 NFHLAKSLDA EVILVSAPEN ETLTELTDRI EIQAQLFGGP RDPKVLGVIL NKVRGEADAA 190 200 210 220 230 240 NAEDGVADFA RRLTEHSPLL RDDFRLIGCI PWQDELNAAR TRDIADLLSA RVINAGDYEQ 250 260 270 280 290 300 RRVQKIVLCA RAVPNTVQLL KPGVLVVTPG DRDDIILAAS LAAMNGVPLA GLLLCSDFPP 310 320 330 340 350 360 DPRIMELCRG ALQGGLPVLS VATGSYDTAT NLNRMNKEIP VDDRERAERV TEFVAGHIDF 370 380 390 400 410 420 EWLKQRCGTP RELRLSPPAF RYQVVQRAQK AGKRIVLPEG SEPRTVQAAA ICQARGIARC 430 440 450 460 470 480 VLLAKPEEVQ AVAQAQGIVL PEGLEIIDPD LVRQRYVEPM VELRKGKGLN APMAEQQLED 490 500 510 520 530 540 SVVLATMMLA LDEVDGLVSG AIHTTASTIR PALQLIKTAP GYNLVSSVFF MLLPDQVLVY 550 560 570 580 590 600 GDCAVNPDPS ASDLAEIAVQ SAASAQAFGI PARVAMISYS TGDSGSGVDV DKVREATRLA 610 620 630 640 650 660 REQRPDLLID GPLQYDAAAI ASVGRQKAPN SPVAGQATVF IFPDLNTGNT TYKAVQRSAD 670 680 690 700 CVSVGPMLQG LRKPVNDLSR GALVEDIVYT IALTAIQADA QAPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Long-term anaerobic survival of the opportunistic pathogen Pseudomonas aeruginosa via pyruvate fermentation." Eschbach M., Schreiber K., Trunk K., Buer J., Jahn D., Schobert M. J. Bacteriol. 186:4596-4604(2004) [PubMed: 15231792] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, INDUCTION, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG04224.1.
PIR	C83541.
RefSeq	NP_249526.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9I5A5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	882123.
GenomeReviews	Gene locus PA0835 in contig AE004091_GR.
KEGG	pae:PA0835.
PATRIC	19835948. VBIPseAer58763_0866.
Organism-specific databases
PseudoCAP	PA0835.
Phylogenomic databases
HOGENOM	HBG576804.
OMA	KPIAQPH.
ProtClustDB	PRK05632.
Enzyme and pathway databases
BioCyc	PAER208964:PA0835-MONOMER.
Family and domain databases
InterPro	IPR002586. CbiA_P_synth. IPR010766. DRTGG. IPR016475. P-Actrans_bac. IPR004614. P_AcTrfase. IPR002505. PTA_PTB. [Graphical view]
KO	K13788.
Pfam	PF01656. CbiA. 1 hit. PF07085. DRTGG. 1 hit. PF01515. PTA_PTB. 1 hit. [Graphical view]
PIRSF	PIRSF006107. PhpActrans_proteobac. 1 hit.
TIGRFAMs	TIGR00651. Pta. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PTA_PSEAE

Accession

Primary (citable) accession number: Q9I5A5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 8, 2011
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program