Reviewed, UniProtKB/Swiss-Prot Q9I587 (FUMC2_PSEAE)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class II 2
      Short name=Fumarase C 2
    EC=4.2.1.2

Gene names

Name:	fumC2
Ordered Locus Names:	PA0854

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle; (S)-malate from fumarate: step 1/1. HAMAP MF_00743
Subunit structure	Homotetramer By similarity. HAMAP MF_00743
Subcellular location	Cytoplasm By similarity HAMAP MF_00743.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity. HAMAP MF_00743
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fumarate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	tricarboxylic acid cycle enzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	Fumarate hydratase class II 2 HAMAP MF_00743		PRO_0000161300
Regions
	Region	127 – 130	4	B site By similarity
	Region	137 – 139	3	Substrate binding By similarity
Sites
	Binding site	98	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I587-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: B0520F835C0DDF61	FASTA	464	49,122
	10 20 30 40 50 60 MSRTETDSLG TIEVPDDAYW GAQTQRSLEN FAIGGQRMPL AVIHALALIK KAAARVNDHL 70 80 90 100 110 120 GELPPEVARL IEQAADEVLA GRHDEHFPLV VWQTGSGTQT NMNVNEVIAG RANELAGNPR 130 140 150 160 170 180 GGKSPVHPND HVNRAQSSND SFPTAMHIAA AKAVHEQLLP AIAELSGGLA EQSARHASLV 190 200 210 220 230 240 KTGRTHLMDA TPITFGQELS AFVAQLDYAE RAIRAALPAV YQLAQGGTAV GTGLNAPKGF 250 260 270 280 290 300 ADAIAAEIAA ESGLPFVAAP NKFAALAGHE PLVILSGALK SLAVALMKIA NDLRLLGSGP 310 320 330 340 350 360 RAGFAEVKLP ANEPGSSIMP GKVNPTQCEA LSMLACQVMG NDSTISFAAS QGHLQLNVFK 370 380 390 400 410 420 PVIVYNLLES IRLLADGCRN FNKHCVAGLE PDAQRMADLL ERGLMLVTAL NPHIGYDKAA 430 440 450 460 EIAKKAYAEG TTLRAAALQL GYLDEAQFDE WVRPEQMLEA GHHG

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG04243.1.
PIR	H83538.
RefSeq	NP_249545.1.
3D structure databases
SMR	Q9I587. Positions 3-458.
ModBase	Search...
Genome annotation databases
GeneID	878044.
GenomeReviews	Gene locus PA0854 in contig AE004091_GR.
KEGG	pae:PA0854.
Organism-specific databases
PseudoCAP	PA0854.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG284369.
OMA	AVCELAQ.
Enzyme and pathway databases
BioCyc	PAER208964:PA0854-MONOMER.
BRENDA	4.2.1.2. 354.
Family and domain databases
HAMAP	MF_00743. FumaraseC. [Tree]
InterPro	IPR003031. D_crystallin. IPR005677. Fum_hydII. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. IPR008948. L-Aspartase-like. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
TIGRFAMs	TIGR00979. fumC_II. 1 hit.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC2_PSEAE

Accession

Primary (citable) accession number: Q9I587

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	March 1, 2001
Last modified:	February 9, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)