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Protein

Fumarate hydratase class II 1

Gene

fumC1

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II 2 (fumC2), Fumarate hydratase class II 1 (fumC1)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei185SubstrateUniRule annotation1
Active sitei186Proton donor/acceptorUniRule annotation1
Active sitei316UniRule annotation1
Binding sitei317SubstrateUniRule annotation1
Sitei329Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processTricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class II 1UniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase C 1UniRule annotation
Alternative name(s):
Aerobic fumarase 1UniRule annotation
Iron-independent fumarase 1UniRule annotation
Gene namesi
Name:fumC1UniRule annotation
Ordered Locus Names:PA0854
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0854.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001613001 – 464Fumarate hydratase class II 1Add BLAST464

Proteomic databases

PaxDbiQ9I587.
PRIDEiQ9I587.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA0854.

Structurei

3D structure databases

ProteinModelPortaliQ9I587.
SMRiQ9I587.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni96 – 98Substrate bindingUniRule annotation3
Regioni127 – 130Substrate binding (B site)UniRule annotation4
Regioni137 – 139Substrate bindingUniRule annotation3
Regioni322 – 324Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q. Bacteria.
COG0114. LUCA.
HOGENOMiHOG000061736.
InParanoidiQ9I587.
KOiK01679.
OMAiVSFTDNC.
PhylomeDBiQ9I587.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9I587-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRTETDSLG TIEVPDDAYW GAQTQRSLEN FAIGGQRMPL AVIHALALIK
60 70 80 90 100
KAAARVNDHL GELPPEVARL IEQAADEVLA GRHDEHFPLV VWQTGSGTQT
110 120 130 140 150
NMNVNEVIAG RANELAGNPR GGKSPVHPND HVNRAQSSND SFPTAMHIAA
160 170 180 190 200
AKAVHEQLLP AIAELSGGLA EQSARHASLV KTGRTHLMDA TPITFGQELS
210 220 230 240 250
AFVAQLDYAE RAIRAALPAV YQLAQGGTAV GTGLNAPKGF ADAIAAEIAA
260 270 280 290 300
ESGLPFVAAP NKFAALAGHE PLVILSGALK SLAVALMKIA NDLRLLGSGP
310 320 330 340 350
RAGFAEVKLP ANEPGSSIMP GKVNPTQCEA LSMLACQVMG NDSTISFAAS
360 370 380 390 400
QGHLQLNVFK PVIVYNLLES IRLLADGCRN FNKHCVAGLE PDAQRMADLL
410 420 430 440 450
ERGLMLVTAL NPHIGYDKAA EIAKKAYAEG TTLRAAALQL GYLDEAQFDE
460
WVRPEQMLEA GHHG
Length:464
Mass (Da):49,122
Last modified:March 1, 2001 - v1
Checksum:iB0520F835C0DDF61
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG04243.1.
PIRiH83538.
RefSeqiNP_249545.1. NC_002516.2.
WP_003085854.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG04243; AAG04243; PA0854.
GeneIDi878044.
KEGGipae:PA0854.
PATRICifig|208964.12.peg.886.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG04243.1.
PIRiH83538.
RefSeqiNP_249545.1. NC_002516.2.
WP_003085854.1. NC_002516.2.

3D structure databases

ProteinModelPortaliQ9I587.
SMRiQ9I587.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0854.

Proteomic databases

PaxDbiQ9I587.
PRIDEiQ9I587.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG04243; AAG04243; PA0854.
GeneIDi878044.
KEGGipae:PA0854.
PATRICifig|208964.12.peg.886.

Organism-specific databases

PseudoCAPiPA0854.

Phylogenomic databases

eggNOGiENOG4105C9Q. Bacteria.
COG0114. LUCA.
HOGENOMiHOG000061736.
InParanoidiQ9I587.
KOiK01679.
OMAiVSFTDNC.
PhylomeDBiQ9I587.

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFUMC1_PSEAE
AccessioniPrimary (citable) accession number: Q9I587
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: March 1, 2001
Last modified: June 7, 2017
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.