Reviewed, UniProtKB/Swiss-Prot Q9I558 (ACSA1_PSEAE)

Names and origin

Protein names

Recommended name:
    Acetyl-coenzyme A synthetase 1
    EC=6.2.1.1
Alternative name(s):
    Acetate--CoA ligase 1
    Acyl-activating enzyme 1

Gene names

Name:	acsA1
Synonyms:	acsA
Ordered Locus Names:	PA0887

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	651 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity. HAMAP MF_01123
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 651	651	Acetyl-coenzyme A synthetase 1 HAMAP MF_01123		PRO_0000208374
Sites
	Active site	517	1	By similarity
Amino acid modifications
	Modified residue	609	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I558-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 991802723539AD23	FASTA	651	71,763
	10 20 30 40 50 60 MSAASLYPVH PEAVARTFTD EATYKTMYQQ SVVNPDGFWR EQAQRIDWIK PFEKVKQTSF 70 80 90 100 110 120 DDHHVDIKWF ADGTLNVSHN CLDRHLAERG DQVAIIWEGD DPADHQEITY RQLHEQVCKF 130 140 150 160 170 180 ANALRGQDVH RGDVVTIYMP MIPEAVVAML ACTRIGAIHS VVFGGFSPEA LAGRIIDCKS 190 200 210 220 230 240 KVVITADEGV RGGKRTPLKA NVDDALTNPE TSSVQKIIVC KRTGAEIKWN QHRDVWYDDL 250 260 270 280 290 300 MKVAGSTCAP KEMGAEDPLF ILYTSGSTGK PKGVLHTTGG YLVYASLTHE RVFDYRPGEV 310 320 330 340 350 360 YWCTADIGWV TGHTYIVYGP LANGATTILF EGVPNYPDVT RVAKIIDKHK VNILYTAPTA 370 380 390 400 410 420 IRAMMAEGKA AVAGADGSSL RLLGSVGEPI NPEAWQWYYE TVGQSRCPIV DTWWQTETGA 430 440 450 460 470 480 CLMTPLPGAH AMKPGSAAKP FFGVVPALVD NLGNLIEGAA EGNLVILDSW PGQARTLFGD 490 500 510 520 530 540 HDRFVDTYFK TFKGMYFTGD GARRDEDGYY WITGRVDDVL NVSGHRMGTA EVESAMVAHP 550 560 570 580 590 600 KVAEAAVVGM QHDIKGQGIY VYVTLNSGVE PSEALRQELK QWVRREIGPI ATPDVIQWAP 610 620 630 640 650 GLPKTRSGKI MRRILRKIAA AEYDTLGDIS TLADPGVVQH LIETHRSMQA A

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG04276.1.
PIR	D83534.
RefSeq	NP_249578.1.
3D structure databases
SMR	Q9I558. Positions 16-647.
ModBase	Search...
Genome annotation databases
GeneID	879732.
GenomeReviews	Gene locus PA0887 in contig AE004091_GR.
KEGG	pae:PA0887.
Organism-specific databases
PseudoCAP	PA0887.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG547964.
OMA	TRVKNTS.
Enzyme and pathway databases
BioCyc	PAER208964:PA0887-MONOMER.
BRENDA	6.2.1.1. 354.
Family and domain databases
HAMAP	MF_01123. Ac_CoA_synth. [Tree]
InterPro	IPR011904. Ac_CoA_lig_AcsA. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA1_PSEAE

Accession

Primary (citable) accession number: Q9I558

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
Last sequence update:	March 1, 2001
Last modified:	February 9, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)