Reviewed, UniProtKB/Swiss-Prot Q9I526 (CYSM_PSEAE)

Names and origin

Protein names

Recommended name:
    Cysteine synthase B
      Short name=CSase B
    EC=2.5.1.47
Alternative name(s):
    O-acetylserine sulfhydrylase B
    O-acetylserine (thiol)-lyase B
      Short name=OAS-TL B

Gene names

Name:	cysM
Ordered Locus Names:	PA0932

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	299 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 299	299	Cysteine synthase B		PRO_0000287759
Regions
	Region	178 – 182	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	75	1	Pyridoxal phosphate By similarity
	Binding site	259	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	45	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I526-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 9B78F6986CD14CC8	FASTA	299	32,448
	10 20 30 40 50 60 MTVQYPTIAD CVGNTPLVRL QRLPGETSNT LLVKLEGNNP AGSVKDRPAL SMITRAELRG 70 80 90 100 110 120 DIRPGDTLIE ATSGNTGIAL AMAAAIKGYK MILIMPDNST AERKAAMTAY GAELILVSKE 130 140 150 160 170 180 EGMEGARDLA DKLQREGRGK VLDQFANGDN PEAHYHSTGP EIWQQTGGSI THFVSSMGTT 190 200 210 220 230 240 GTIMGVSRYL KEQNPAVQIV GLQPMEGSAI PGIRRWPQEY LPKIYDASRV DRVVDMHQDE 250 260 270 280 290 AEDIMRRLAR EEGIFCGVSS GGAVAAMLRL SRELENAVLV AIICDRGDRY LSSGVYDPR

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004091 Genomic DNA. Translation: AAG04321.1.
PIR	A83530.
RefSeq	NP_249623.1.
3D structure databases
HSSP	HSSP built from PDB template 1O58 based on UniProtKB Q9WZD3.
ModBase	Search...
Genome annotation databases
GeneID	881832.
GenomeReviews	Gene locus PA0932 in contig AE004091_GR.
KEGG	pae:PA0932.
Organism-specific databases
PseudoCAP	PA0932.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9I526.
OMA	DRAALNM.
Enzyme and pathway databases
BioCyc	PAER208964:PA0932-MON.
BRENDA	2.5.1.47. 354.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005858. CysM. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
TIGRFAMs	TIGR01136. cysKM. 1 hit. TIGR01138. cysM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSM_PSEAE

Accession

Primary (citable) accession number: Q9I526

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)