Q9I513 (PUR5_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	PA0945

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741_B
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00741_B.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 353	353	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741_B		PRO_0000148233

Sequences

	Sequence		Length	Mass (Da)
	Q9I513 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: F50169F97482E553	FASTA	353	37,123
	10 20 30 40 50 60 MSSKQPSLSY KDAGVDIDAG EALVERIKGV AKRTARPEVM GGLGGFGALC EIPAGYKQPV 70 80 90 100 110 120 LVSGTDGVGT KLRLALNLNK HDSIGQDLVA MCVNDLVVCG AEPLFFLDYY ATGKLNVDVA 130 140 150 160 170 180 ATVVTGIGAG CELAGCSLVG GETAEMPGMY EGEDYDLAGF CVGVVEKAEI IDGSRVQAGD 190 200 210 220 230 240 ALIALPSSGP HSNGYSLIRK IIEVSGADIA QVQLDGKPLA DLLMAPTRIY VKPLLQLIKQ 250 260 270 280 290 300 TGAVKAMAHI TGGGLLDNIP RVLPDNAQAV IDVASWNRPA VFDWLQEQGN VDETEMHRVL 310 320 330 340 350 NCGVGMVICV AQSDAEKALE VLRAAGEQPW QIGRIETCGA DAERVVLNNL KNH

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG04334.1.
PIR	A83529.
RefSeq	NP_249636.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9I513.
SMR	Q9I513. Positions 7-347.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	879549.
GenomeReviews	Gene locus PA0945 in contig AE004091_GR.
KEGG	pae:PA0945.
NMPDR	fig|208964.1.peg.946.
PATRIC	19836186. VBIPseAer58763_0981.
Organism-specific databases
PseudoCAP	PA0945.
Phylogenomic databases
HOGENOM	HBG531222.
OMA	PENIPRV.
ProtClustDB	PRK05385.
Enzyme and pathway databases
BioCyc	PAER208964:PA0945-MONOMER.
Family and domain databases
HAMAP	MF_00741_B. AIRS_B. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
KO	K01933.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. PurM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_PSEAE

Accession

Primary (citable) accession number: Q9I513

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program