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Protein

NAD(P)H dehydrogenase (quinone)

Gene

PA0949

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

It seems to function in response to environmental stress when various electron transfer chains are affected or when the environment is highly oxidizing. It reduces quinones to the hydroquinone state to prevent interaction of the semiquinone with O2 and production of superoxide (By similarity).By similarity

Catalytic activityi

NAD(P)H + a quinone = NAD(P)+ + a hydroquinone.

Cofactori

FMN1 PublicationNote: Binds 1 FMN per monomer.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei81 – 811FMN; via amide nitrogen1 Publication
Binding sitei99 – 991SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 176FMN1 Publication
Nucleotide bindingi114 – 1207FMN1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Flavoprotein, FMN, NAD, NADP, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
NAD(P)H dehydrogenase (quinone) (EC:1.6.5.2)
Alternative name(s):
Flavoprotein WrbA
NAD(P)H:quinone oxidoreductase
Short name:
NQO
Gene namesi
Ordered Locus Names:PA0949
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA0949.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 198197NAD(P)H dehydrogenase (quinone)PRO_0000422322Add
BLAST

Proteomic databases

PaxDbiQ9I509.

Interactioni

Subunit structurei

Homodimer.2 Publications

Protein-protein interaction databases

STRINGi208964.PA0949.

Structurei

Secondary structure

1
198
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 106Combined sources
Beta strandi13 – 153Combined sources
Helixi16 – 3015Combined sources
Beta strandi34 – 396Combined sources
Helixi64 – 685Combined sources
Beta strandi71 – 788Combined sources
Helixi86 – 938Combined sources
Helixi96 – 1016Combined sources
Turni102 – 1065Combined sources
Beta strandi108 – 1169Combined sources
Helixi122 – 13514Combined sources
Beta strandi160 – 1656Combined sources
Turni167 – 1693Combined sources
Helixi176 – 19722Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZWKX-ray2.60A/B2-196[»]
1ZWLX-ray2.80A2-196[»]
2A5LX-ray1.70A/B1-198[»]
ProteinModelPortaliQ9I509.
SMRiQ9I509. Positions 3-198.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9I509.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 190185Flavodoxin-likeAdd
BLAST

Sequence similaritiesi

Belongs to the WrbA family.Curated
Contains 1 flavodoxin-like domain.Curated

Phylogenomic databases

eggNOGiENOG4105CS1. Bacteria.
COG0655. LUCA.
HOGENOMiHOG000030539.
InParanoidiQ9I509.
KOiK03809.
OMAiMMMPLLH.
PhylomeDBiQ9I509.

Family and domain databases

Gene3Di3.40.50.360. 1 hit.
HAMAPiMF_01017. NQOR. 1 hit.
InterProiIPR008254. Flavodoxin/NO_synth.
IPR001226. Flavodoxin_CS.
IPR029039. Flavoprotein-like_dom.
IPR010089. Flavoprotein_WrbA.
IPR005025. FMN_Rdtase-like.
[Graphical view]
PfamiPF03358. FMN_red. 1 hit.
[Graphical view]
SUPFAMiSSF52218. SSF52218. 1 hit.
TIGRFAMsiTIGR01755. flav_wrbA. 1 hit.
PROSITEiPS00201. FLAVODOXIN. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9I509-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSPYILVLY YSRHGATAEM ARQIARGVEQ GGFEARVRTV PAVSTECEAV
60 70 80 90 100
APDIPAEGAL YATLEDLKNC AGLALGSPTR FGNMASPLKY FLDGTSSLWL
110 120 130 140 150
TGSLVGKPAA VFTSTASLHG GQETTQLSML LPLLHHGMLV LGIPYSEPAL
160 170 180 190
LETRGGGTPY GASHFAGADG KRSLDEHELT LCRALGKRLA ETAGKLGS
Length:198
Mass (Da):20,763
Last modified:March 1, 2001 - v1
Checksum:iDA5551A18259FD70
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG04338.1.
PIRiD83526.
RefSeqiNP_249640.1. NC_002516.2.
WP_003115189.1. NZ_ASJY01000132.1.

Genome annotation databases

EnsemblBacteriaiAAG04338; AAG04338; PA0949.
GeneIDi882021.
KEGGipae:PA0949.
PATRICi19836196. VBIPseAer58763_0986.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG04338.1.
PIRiD83526.
RefSeqiNP_249640.1. NC_002516.2.
WP_003115189.1. NZ_ASJY01000132.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZWKX-ray2.60A/B2-196[»]
1ZWLX-ray2.80A2-196[»]
2A5LX-ray1.70A/B1-198[»]
ProteinModelPortaliQ9I509.
SMRiQ9I509. Positions 3-198.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA0949.

Proteomic databases

PaxDbiQ9I509.

Protocols and materials databases

DNASUi882021.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG04338; AAG04338; PA0949.
GeneIDi882021.
KEGGipae:PA0949.
PATRICi19836196. VBIPseAer58763_0986.

Organism-specific databases

PseudoCAPiPA0949.

Phylogenomic databases

eggNOGiENOG4105CS1. Bacteria.
COG0655. LUCA.
HOGENOMiHOG000030539.
InParanoidiQ9I509.
KOiK03809.
OMAiMMMPLLH.
PhylomeDBiQ9I509.

Miscellaneous databases

EvolutionaryTraceiQ9I509.

Family and domain databases

Gene3Di3.40.50.360. 1 hit.
HAMAPiMF_01017. NQOR. 1 hit.
InterProiIPR008254. Flavodoxin/NO_synth.
IPR001226. Flavodoxin_CS.
IPR029039. Flavoprotein-like_dom.
IPR010089. Flavoprotein_WrbA.
IPR005025. FMN_Rdtase-like.
[Graphical view]
PfamiPF03358. FMN_red. 1 hit.
[Graphical view]
SUPFAMiSSF52218. SSF52218. 1 hit.
TIGRFAMsiTIGR01755. flav_wrbA. 1 hit.
PROSITEiPS00201. FLAVODOXIN. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNQOR_PSEAE
AccessioniPrimary (citable) accession number: Q9I509
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2013
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.