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Protein

Nitronate monooxygenase

Gene

PA1024

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative denitrification of nitronates to their corresponding aldehydes and nitrites.1 Publication

Catalytic activityi

Ethylnitronate + O2 = acetaldehyde + nitrite + other products.

Cofactori

FMN1 PublicationNote: Binds 1 FMN per subunit.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei75 – 751FMN1 Publication
Binding sitei124 – 1241FMN1 Publication
Binding sitei150 – 1501FMN; via amide nitrogen1 Publication
Active sitei152 – 1521Proton acceptor1 Publication
Binding sitei152 – 1521Substrate1 Publication
Binding sitei288 – 2881Substrate1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 243FMN1 Publication
Nucleotide bindingi178 – 1803FMN1 Publication
Nucleotide bindingi201 – 2022FMN1 Publication

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

BRENDAi1.13.12.16. 5087.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitronate monooxygenase (EC:1.13.12.16)
Alternative name(s):
Nitroalkane oxidase
Gene namesi
Ordered Locus Names:PA1024
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA1024.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Nitronate monooxygenasePRO_0000392207Add
BLAST

Proteomic databases

PaxDbiQ9I4V0.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi208964.PA1024.

Structurei

Secondary structure

1
328
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 126Combined sources
Beta strandi15 – 206Combined sources
Turni24 – 263Combined sources
Helixi29 – 379Combined sources
Beta strandi43 – 453Combined sources
Turni46 – 483Combined sources
Beta strandi49 – 513Combined sources
Helixi52 – 6514Combined sources
Beta strandi71 – 766Combined sources
Helixi85 – 9410Combined sources
Beta strandi99 – 1057Combined sources
Helixi108 – 1169Combined sources
Beta strandi120 – 1278Combined sources
Helixi128 – 1369Combined sources
Beta strandi140 – 1456Combined sources
Helixi160 – 1689Combined sources
Beta strandi175 – 1806Combined sources
Helixi184 – 19310Combined sources
Beta strandi196 – 2016Combined sources
Helixi202 – 2054Combined sources
Beta strandi207 – 2104Combined sources
Helixi214 – 2229Combined sources
Beta strandi228 – 2314Combined sources
Helixi233 – 2353Combined sources
Beta strandi239 – 2424Combined sources
Helixi245 – 25511Combined sources
Helixi261 – 2633Combined sources
Helixi265 – 2684Combined sources
Helixi270 – 27910Combined sources
Helixi291 – 2955Combined sources
Helixi302 – 31817Combined sources
Helixi320 – 3256Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GJLX-ray2.00A1-328[»]
2GJNX-ray2.30A1-328[»]
ProteinModelPortaliQ9I4V0.
SMRiQ9I4V0. Positions 3-326.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9I4V0.

Family & Domainsi

Sequence similaritiesi

Belongs to the nitronate monooxygenase family.Curated

Phylogenomic databases

eggNOGiENOG4105EA8. Bacteria.
COG2070. LUCA.
HOGENOMiHOG000123285.
InParanoidiQ9I4V0.
KOiK00459.
OMAiVLHKCTT.
PhylomeDBiQ9I4V0.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR004136. NMO.
[Graphical view]
PfamiPF03060. NMO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9I4V0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVFRTRFTE TFGVEHPIMQ GGMQWVGRAE MAAAVANAGG LATLSALTQP
60 70 80 90 100
SPEALAAEIA RCRELTDRPF GVNLTLLPTQ KPVPYAEYRA AIIEAGIRVV
110 120 130 140 150
ETAGNDPGEH IAEFRRHGVK VIHKCTAVRH ALKAERLGVD AVSIDGFECA
160 170 180 190 200
GHPGEDDIPG LVLLPAAANR LRVPIIASGG FADGRGLVAA LALGADAINM
210 220 230 240 250
GTRFLATREC PIHPAVKAAI RAADERSTDL IMRSLRNTAR VARNAISQEV
260 270 280 290 300
LAIEARGGAG YADIAALVSG QRGRQVYQQG DTDLGIWSAG MVQGLIDDEP
310 320
ACAELLRDIV EQARQLVRQR LEGMLAGV
Length:328
Mass (Da):34,819
Last modified:March 1, 2001 - v1
Checksum:i57A9B16894527E8B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG04413.1.
PIRiA83519.
RefSeqiNP_249715.1. NC_002516.2.
WP_003086306.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG04413; AAG04413; PA1024.
GeneIDi882956.
KEGGipae:PA1024.
PATRICi19836344. VBIPseAer58763_1058.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG04413.1.
PIRiA83519.
RefSeqiNP_249715.1. NC_002516.2.
WP_003086306.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GJLX-ray2.00A1-328[»]
2GJNX-ray2.30A1-328[»]
ProteinModelPortaliQ9I4V0.
SMRiQ9I4V0. Positions 3-326.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA1024.

Proteomic databases

PaxDbiQ9I4V0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG04413; AAG04413; PA1024.
GeneIDi882956.
KEGGipae:PA1024.
PATRICi19836344. VBIPseAer58763_1058.

Organism-specific databases

PseudoCAPiPA1024.

Phylogenomic databases

eggNOGiENOG4105EA8. Bacteria.
COG2070. LUCA.
HOGENOMiHOG000123285.
InParanoidiQ9I4V0.
KOiK00459.
OMAiVLHKCTT.
PhylomeDBiQ9I4V0.

Enzyme and pathway databases

BRENDAi1.13.12.16. 5087.

Miscellaneous databases

EvolutionaryTraceiQ9I4V0.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR004136. NMO.
[Graphical view]
PfamiPF03060. NMO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei2NPD_PSEAE
AccessioniPrimary (citable) accession number: Q9I4V0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.