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Protein

Nitronate monooxygenase

Gene

PA1024

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative denitrification of nitronates to their corresponding aldehydes and nitrites.1 Publication

Catalytic activityi

Ethylnitronate + O2 = acetaldehyde + nitrite + other products.

Cofactori

FMN1 PublicationNote: Binds 1 FMN per subunit.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei75FMN1 Publication1
Binding sitei124FMN1 Publication1
Binding sitei150FMN; via amide nitrogen1 Publication1
Active sitei152Proton acceptor1 Publication1
Binding sitei152Substrate1 Publication1
Binding sitei288Substrate1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi22 – 24FMN1 Publication3
Nucleotide bindingi178 – 180FMN1 Publication3
Nucleotide bindingi201 – 202FMN1 Publication2

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

BRENDAi1.13.12.16. 5087.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitronate monooxygenase (EC:1.13.12.16)
Alternative name(s):
Nitroalkane oxidase
Gene namesi
Ordered Locus Names:PA1024
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA1024.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003922071 – 328Nitronate monooxygenaseAdd BLAST328

Proteomic databases

PaxDbiQ9I4V0.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi208964.PA1024.

Structurei

Secondary structure

1328
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 12Combined sources6
Beta strandi15 – 20Combined sources6
Turni24 – 26Combined sources3
Helixi29 – 37Combined sources9
Beta strandi43 – 45Combined sources3
Turni46 – 48Combined sources3
Beta strandi49 – 51Combined sources3
Helixi52 – 65Combined sources14
Beta strandi71 – 76Combined sources6
Helixi85 – 94Combined sources10
Beta strandi99 – 105Combined sources7
Helixi108 – 116Combined sources9
Beta strandi120 – 127Combined sources8
Helixi128 – 136Combined sources9
Beta strandi140 – 145Combined sources6
Helixi160 – 168Combined sources9
Beta strandi175 – 180Combined sources6
Helixi184 – 193Combined sources10
Beta strandi196 – 201Combined sources6
Helixi202 – 205Combined sources4
Beta strandi207 – 210Combined sources4
Helixi214 – 222Combined sources9
Beta strandi228 – 231Combined sources4
Helixi233 – 235Combined sources3
Beta strandi239 – 242Combined sources4
Helixi245 – 255Combined sources11
Helixi261 – 263Combined sources3
Helixi265 – 268Combined sources4
Helixi270 – 279Combined sources10
Helixi291 – 295Combined sources5
Helixi302 – 318Combined sources17
Helixi320 – 325Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GJLX-ray2.00A1-328[»]
2GJNX-ray2.30A1-328[»]
ProteinModelPortaliQ9I4V0.
SMRiQ9I4V0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9I4V0.

Family & Domainsi

Sequence similaritiesi

Belongs to the nitronate monooxygenase family.Curated

Phylogenomic databases

eggNOGiENOG4105EA8. Bacteria.
COG2070. LUCA.
HOGENOMiHOG000123285.
InParanoidiQ9I4V0.
KOiK00459.
OMAiVLHKCTT.
PhylomeDBiQ9I4V0.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR004136. NMO.
[Graphical view]
PfamiPF03060. NMO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9I4V0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVFRTRFTE TFGVEHPIMQ GGMQWVGRAE MAAAVANAGG LATLSALTQP
60 70 80 90 100
SPEALAAEIA RCRELTDRPF GVNLTLLPTQ KPVPYAEYRA AIIEAGIRVV
110 120 130 140 150
ETAGNDPGEH IAEFRRHGVK VIHKCTAVRH ALKAERLGVD AVSIDGFECA
160 170 180 190 200
GHPGEDDIPG LVLLPAAANR LRVPIIASGG FADGRGLVAA LALGADAINM
210 220 230 240 250
GTRFLATREC PIHPAVKAAI RAADERSTDL IMRSLRNTAR VARNAISQEV
260 270 280 290 300
LAIEARGGAG YADIAALVSG QRGRQVYQQG DTDLGIWSAG MVQGLIDDEP
310 320
ACAELLRDIV EQARQLVRQR LEGMLAGV
Length:328
Mass (Da):34,819
Last modified:March 1, 2001 - v1
Checksum:i57A9B16894527E8B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG04413.1.
PIRiA83519.
RefSeqiNP_249715.1. NC_002516.2.
WP_003086306.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG04413; AAG04413; PA1024.
GeneIDi882956.
KEGGipae:PA1024.
PATRICi19836344. VBIPseAer58763_1058.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG04413.1.
PIRiA83519.
RefSeqiNP_249715.1. NC_002516.2.
WP_003086306.1. NC_002516.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GJLX-ray2.00A1-328[»]
2GJNX-ray2.30A1-328[»]
ProteinModelPortaliQ9I4V0.
SMRiQ9I4V0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA1024.

Proteomic databases

PaxDbiQ9I4V0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG04413; AAG04413; PA1024.
GeneIDi882956.
KEGGipae:PA1024.
PATRICi19836344. VBIPseAer58763_1058.

Organism-specific databases

PseudoCAPiPA1024.

Phylogenomic databases

eggNOGiENOG4105EA8. Bacteria.
COG2070. LUCA.
HOGENOMiHOG000123285.
InParanoidiQ9I4V0.
KOiK00459.
OMAiVLHKCTT.
PhylomeDBiQ9I4V0.

Enzyme and pathway databases

BRENDAi1.13.12.16. 5087.

Miscellaneous databases

EvolutionaryTraceiQ9I4V0.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR004136. NMO.
[Graphical view]
PfamiPF03060. NMO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei2NPD_PSEAE
AccessioniPrimary (citable) accession number: Q9I4V0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2010
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.