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Q9I4K6

- FOSA_PSEAE

UniProt

Q9I4K6 - FOSA_PSEAE

Protein

Glutathione transferase FosA

Gene

fosA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Metalloglutathione transferase which confers resistance to fosfomycin by catalyzing the addition of glutathione to fosfomycin.1 Publication

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.

    Cofactori

    Manganese.1 Publication

    Enzyme regulationi

    Requires the monovalent cation K+ for optimal activity.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi7 – 71Manganese
    Metal bindingi64 – 641Manganese
    Metal bindingi110 – 1101Manganese

    GO - Molecular functioni

    1. glutathione transferase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Antibiotic resistance

    Keywords - Ligandi

    Manganese, Metal-binding, Potassium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione transferase FosA (EC:2.5.1.18)
    Alternative name(s):
    Fosfomycin resistance protein
    Gene namesi
    Name:fosA
    Ordered Locus Names:PA1129
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000002438: Chromosome

    Organism-specific databases

    PseudoCAPiPA1129.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 135135Glutathione transferase FosAPRO_0000164043Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi208964.PA1129.

    Structurei

    Secondary structure

    1
    135
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi2 – 1312
    Helixi15 – 2410
    Beta strandi29 – 346
    Beta strandi37 – 426
    Beta strandi45 – 517
    Beta strandi60 – 623
    Beta strandi64 – 696
    Helixi71 – 733
    Helixi74 – 8310
    Beta strandi88 – 903
    Beta strandi94 – 963
    Beta strandi98 – 1025
    Beta strandi108 – 1136
    Helixi116 – 12510

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1LQKX-ray1.35A/B1-135[»]
    1LQOX-ray2.00A/B1-135[»]
    1LQPX-ray1.19A/B1-135[»]
    1NKIX-ray0.95A/B1-135[»]
    1NNRX-ray2.25A/B1-135[»]
    ProteinModelPortaliQ9I4K6.
    SMRiQ9I4K6. Positions 1-134.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9I4K6.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0346.
    HOGENOMiHOG000232023.
    OMAiHYAFSIA.
    OrthoDBiEOG6VXFB2.
    PhylomeDBiQ9I4K6.

    Family and domain databases

    Gene3Di3.10.180.10. 1 hit.
    InterProiIPR029068. Glyas_Bleomycin-R_OHBP_Dase.
    IPR025870. Glyoxalase-like_dom.
    [Graphical view]
    PfamiPF12681. Glyoxalase_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF54593. SSF54593. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9I4K6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLTGLNHLTL AVADLPASIA FYRDLLGFRL EARWDQGAYL ELGSLWLCLS    50
    REPQYGGPAA DYTHYAFGIA AADFARFAAQ LRAHGVREWK QNRSEGDSFY 100
    FLDPDGHRLE AHVGDLRSRL AACRQAPYAG MRFAD 135
    Length:135
    Mass (Da):15,124
    Last modified:March 1, 2001 - v1
    Checksum:i0D75F174291A2366
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG04518.1.
    PIRiE83503.
    RefSeqiNP_249820.1. NC_002516.2.

    Genome annotation databases

    EnsemblBacteriaiAAG04518; AAG04518; PA1129.
    GeneIDi877785.
    KEGGipae:PA1129.
    PATRICi19836576. VBIPseAer58763_1174.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE004091 Genomic DNA. Translation: AAG04518.1 .
    PIRi E83503.
    RefSeqi NP_249820.1. NC_002516.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1LQK X-ray 1.35 A/B 1-135 [» ]
    1LQO X-ray 2.00 A/B 1-135 [» ]
    1LQP X-ray 1.19 A/B 1-135 [» ]
    1NKI X-ray 0.95 A/B 1-135 [» ]
    1NNR X-ray 2.25 A/B 1-135 [» ]
    ProteinModelPortali Q9I4K6.
    SMRi Q9I4K6. Positions 1-134.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 208964.PA1129.

    Protocols and materials databases

    DNASUi 877785.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG04518 ; AAG04518 ; PA1129 .
    GeneIDi 877785.
    KEGGi pae:PA1129.
    PATRICi 19836576. VBIPseAer58763_1174.

    Organism-specific databases

    PseudoCAPi PA1129.

    Phylogenomic databases

    eggNOGi COG0346.
    HOGENOMi HOG000232023.
    OMAi HYAFSIA.
    OrthoDBi EOG6VXFB2.
    PhylomeDBi Q9I4K6.

    Miscellaneous databases

    EvolutionaryTracei Q9I4K6.

    Family and domain databases

    Gene3Di 3.10.180.10. 1 hit.
    InterProi IPR029068. Glyas_Bleomycin-R_OHBP_Dase.
    IPR025870. Glyoxalase-like_dom.
    [Graphical view ]
    Pfami PF12681. Glyoxalase_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54593. SSF54593. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
    2. "Crystal structure of a genomically encoded fosfomycin resistance protein (FosA) at 1.19 A resolution by MAD phasing off the L-III edge of Tl(+)."
      Rife C.L., Pharris R.E., Newcomer M.E., Armstrong R.N.
      J. Am. Chem. Soc. 124:11001-11003(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.19 ANGSTROMS) IN COMPLEX WITH FOSFOMYCIN, FUNCTION, COFACTOR.
      Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

    Entry informationi

    Entry nameiFOSA_PSEAE
    AccessioniPrimary (citable) accession number: Q9I4K6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 13, 2004
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3