Reviewed,
UniProtKB/Swiss-Prot Q9I4G8 (LOXA_PSEAE)
Last modified
June 16, 2009.
Version 49.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (1) |
 Third-party data |
 Customize display | text xml rdf/xml gff fasta |
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: Arachidonate 15-lipoxygenase Short name=15-LOX EC=1.13.11.33 | ||||
| Gene names |
| ||||
| Organism | Pseudomonas aeruginosa [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 287 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas |
Protein attributes
| Sequence length | 685 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Converts arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid (HpETE) which is rapidly reduced to hydroxyeicosatetraenoic acid (HETE). Could act on exogenous human-derived substrates to potentially modulate the local inflammatory responses during P.aeruginosa infections. Ref.2 |
| Catalytic activity | Arachidonate + O2 = (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate. |
| Cofactor | Binds 1 iron ion per subunit By similarity. |
| Subcellular location | Periplasm. Secreted › extracellular space. Note: Periplasmic with some activity further secreted to the extracellular milieu. |
| Sequence similarities | Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Periplasm Secreted |
| Domain | Signal |
| Ligand | Iron Metal-binding |
| Molecular function | Dioxygenase Oxidoreductase |
| Technical term | Complete proteome |
| Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell periplasmic spaceInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | arachidonate 15-lipoxygenase activity Inferred from electronic annotation. Source: EC iron ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 19 | 19 | Potential | ||||||
| Chain | 20 – 685 | 666 | Arachidonate 15-lipoxygenase | PRO_0000018329 | |||||
Regions | |||||||||
| Domain | 122 – 685 | 564 | Lipoxygenase | ||||||
Sites | |||||||||
| Metal binding | 377 | 1 | Iron; catalytic By similarity | ||||||
| Metal binding | 382 | 1 | Iron; catalytic By similarity | ||||||
| Metal binding | 555 | 1 | Iron; catalytic By similarity | ||||||
| Metal binding | 559 | 1 | Iron; catalytic By similarity | ||||||
| Metal binding | 685 | 1 | Iron; via carboxylate; catalytic By similarity | ||||||
Sequences
| ||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R.  Olson M.V.Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228. |
| [2] | "The opportunistic pathogen Pseudomonas aeruginosa carries a secretable arachidonate 15-lipoxygenase." Vance R.E., Hong S., Gronert K., Serhan C.N., Mekalanos J.J. Proc. Natl. Acad. Sci. U.S.A. 101:2135-2139(2004) [PubMed: 14766977] [Abstract] Cited for: FUNCTION. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228. |
Cross-references
Sequence databases | |
|---|---|
| AE004091 Genomic DNA. Translation: AAG04558.1. | |
| PIR | A83499. |
| RefSeq | NP_249860.1. |
3D structure databases | |
| HSSP | HSSP built from PDB template 2SBL based on UniProtKB P08170. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 879547. |
| GenomeReviews | Gene locus PA1169 in contig AE004091_GR. |
| KEGG | pae:PA1169. |
| NMPDR | fig|208964.1.peg.1170. |
Organism-specific databases | |
| PseudoCAP | PA1169. |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | Q9I4G8. |
| OMA | Q9I4G8. MRFHEDK. |
Enzyme and pathway databases | |
| BioCyc | PAER208964:PA1169-MON. |
| BRENDA | 1.13.11.33. 354. |
Family and domain databases | |
| InterPro | IPR000907. LipOase. IPR013819. LipOase_C. [Graphical view] |
| PANTHER | PTHR11771. LipOase. 1 hit. |
| Pfam | PF00305. Lipoxygenase. 3 hits. [Graphical view] |
| PRINTS | PR00087. LIPOXYGENASE. |
| PROSITE | PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | LOXA_PSEAE | ||||||||
| Accession | Primary (citable) accession number: Q9I4G8 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
