Periplasmic nitrate reductase precursor - Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Preferred order of sections

Names and origin

Protein names

Recommended name:
Periplasmic nitrate reductase
EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	PA1174

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	829 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP MF_01630
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP MF_01630
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_01630 Binds 1 molybdenum ion per subunit By similarity. HAMAP MF_01630 Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity.
Subunit structure	Interacts with napB By similarity. HAMAP MF_01630
Subcellular location	Periplasm By similarity HAMAP MF_01630.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: InterPro nitrate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

24

Potential

Chain

25 – 829

805

Periplasmic nitrate reductase HAMAP MF_01630

PRO_0000045995

Sites

Metal binding

43

1

Iron-sulfur (4Fe-4S) By similarity

Metal binding

46

1

Iron-sulfur (4Fe-4S) By similarity

Metal binding

50

1

Iron-sulfur (4Fe-4S) By similarity

Metal binding

78

1

Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q9I4G3 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 4B543C34FEDB0398
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FASTA

829

92,975

        10         20         30         40         50         60 
MNSPRPTPPP FAAAAAGLPI LVRASNLVTE ADVTSLVWNK APCRFCGTGC SVMVATRDGQ 

        70         80         90        100        110        120 
VVATHGDIKA EVNRGINCVK GYFLSKIMYG SDRLTRPLLR MKDGKFDKQG EFQPISWEQA 

       130        140        150        160        170        180 
FDIMAEKFKA ALKAKGPESV GMFGSGQWTV WEGYAANKLF KAGLRSNNID PNARHCMASA 

       190        200        210        220        230        240 
VMGFMRSFGM DEPMGCYDDI EATDSFVLWG SNMAEMHPVL WSRVTDRRLS APQVKVAVLS 

       250        260        270        280        290        300 
TFEHRSFELA DLPMVFKPQT DLIILNYIAN HIIESGAVNR DFVERHVRFA HGAEDIGYGL 

       310        320        330        340        350        360 
RPDDPLEKKA KNADKANTWS DIDFKAFAEF VKPYTLERTA RESGVPAERL KALAELYADP 

       370        380        390        400        410        420 
KRKVVSFWTM GFNQHTRGVW ANNLIYNIHL LTGKISEPGN SPFSLTGQPS ACGTAREVGT 

       430        440        450        460        470        480 
FSHRLPADLV VTNPKHRETA EKIWKVPAGT IQEKVGFHAV QQSRMLKDGV LNVYWTQVSN 

       490        500        510        520        530        540 
NMQAGPNVMQ EVLPGWRNPD NFVIVSDVYP TVSAQAADLI LPSAMWVEKE GAFGNAERRT 

       550        560        570        580        590        600 
QFWHQLVKAP GEAKSDLWQL VEFSKRFTTD EVWPAELLAK APELKGKTLY DVLFRNGQVD 

       610        620        630        640        650        660 
RFPASDLAKG YANDEVDAFG FYIQKGLFEE YAAFGRGHGH DLAPFDAYHE ARGLRWPVVD 

       670        680        690        700        710        720 
GKETRWRYRE GYDPYVSKGS GVQFYGYPDK KAIVFALPYE PPAEAPDQDY PFWLATGRVL 

       730        740        750        760        770        780 
EHWHTGSMTA RVPELYKAVP DALVYMHPED ARQLKLRRGS EVKVVSRRGE IRARVETRGR 

       790        800        810        820 
NKPPQGLVFV PFFDANKLIN KVTLDATDPI SKQTDYKKCA VRIELLNLA

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References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen."
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R.

Olson M.V.
Nature 406:959-964(2000) [PubMed: 10984043] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG04563.1.
PIR	F83499.
RefSeq	NP_249865.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9I4G3.
SMR	Q9I4G3. Positions 38-824.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	878409.
GenomeReviews	Gene locus PA1174 in contig AE004091_GR.
KEGG	pae:PA1174.
NMPDR	fig\|208964.1.peg.1175.
PATRIC	19836666. VBIPseAer58763_1219.
Organism-specific databases
PseudoCAP	PA1174.
Phylogenomic databases
HOGENOM	HBG715033.
OMA	QFWHQLV.
ProtClustDB	PRK13532.
Enzyme and pathway databases
BioCyc	PAER208964:PA1174-MONOMER.
Family and domain databases
HAMAP	MF_01630. Nitrate_reduct. [Tree]
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. IPR010051. Periplasm_NO3_reductase_lsu. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
KO	K02567.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
SMART	SM00926. Molybdop_Fe4S4. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
TIGRFAMs	TIGR01706. NAPA. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_PSEAE

Accession

Primary (citable) accession number: Q9I4G3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents