Reviewed, UniProtKB/Swiss-Prot Q9I4G3 (NAPA_PSEAE)

Names and origin

Protein names

Recommended name:
    Periplasmic nitrate reductase
    EC=1.7.99.4

Gene names

Name:	napA
Ordered Locus Names:	PA1174

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	829 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP MF_01630
Catalytic activity	Nitrite + acceptor = nitrate + reduced acceptor. HAMAP MF_01630
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_01630 Binds 1 molybdenum ion per subunit By similarity. HAMAP MF_01630 Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit By similarity. HAMAP MF_01630
Subunit structure	Interacts with napB By similarity. HAMAP MF_01630
Subcellular location	Periplasm By similarity HAMAP MF_01630.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Ontologies

Keywords
Biological process	Electron transport Nitrate assimilation Transport
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Mo-molybdopterin cofactor biosynthetic process Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 829	805	Periplasmic nitrate reductase HAMAP MF_01630		PRO_0000045995
Sites
	Metal binding	43	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	46	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	50	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	78	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I4G3-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 4B543C34FEDB0398	FASTA	829	92,975
	10 20 30 40 50 60 MNSPRPTPPP FAAAAAGLPI LVRASNLVTE ADVTSLVWNK APCRFCGTGC SVMVATRDGQ 70 80 90 100 110 120 VVATHGDIKA EVNRGINCVK GYFLSKIMYG SDRLTRPLLR MKDGKFDKQG EFQPISWEQA 130 140 150 160 170 180 FDIMAEKFKA ALKAKGPESV GMFGSGQWTV WEGYAANKLF KAGLRSNNID PNARHCMASA 190 200 210 220 230 240 VMGFMRSFGM DEPMGCYDDI EATDSFVLWG SNMAEMHPVL WSRVTDRRLS APQVKVAVLS 250 260 270 280 290 300 TFEHRSFELA DLPMVFKPQT DLIILNYIAN HIIESGAVNR DFVERHVRFA HGAEDIGYGL 310 320 330 340 350 360 RPDDPLEKKA KNADKANTWS DIDFKAFAEF VKPYTLERTA RESGVPAERL KALAELYADP 370 380 390 400 410 420 KRKVVSFWTM GFNQHTRGVW ANNLIYNIHL LTGKISEPGN SPFSLTGQPS ACGTAREVGT 430 440 450 460 470 480 FSHRLPADLV VTNPKHRETA EKIWKVPAGT IQEKVGFHAV QQSRMLKDGV LNVYWTQVSN 490 500 510 520 530 540 NMQAGPNVMQ EVLPGWRNPD NFVIVSDVYP TVSAQAADLI LPSAMWVEKE GAFGNAERRT 550 560 570 580 590 600 QFWHQLVKAP GEAKSDLWQL VEFSKRFTTD EVWPAELLAK APELKGKTLY DVLFRNGQVD 610 620 630 640 650 660 RFPASDLAKG YANDEVDAFG FYIQKGLFEE YAAFGRGHGH DLAPFDAYHE ARGLRWPVVD 670 680 690 700 710 720 GKETRWRYRE GYDPYVSKGS GVQFYGYPDK KAIVFALPYE PPAEAPDQDY PFWLATGRVL 730 740 750 760 770 780 EHWHTGSMTA RVPELYKAVP DALVYMHPED ARQLKLRRGS EVKVVSRRGE IRARVETRGR 790 800 810 820 NKPPQGLVFV PFFDANKLIN KVTLDATDPI SKQTDYKKCA VRIELLNLA

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG04563.1.
PIR	F83499.
RefSeq	NP_249865.1.
3D structure databases
SMR	Q9I4G3. Positions 38-824.
ModBase	Search...
Genome annotation databases
GeneID	878409.
GenomeReviews	Gene locus PA1174 in contig AE004091_GR.
KEGG	pae:PA1174.
NMPDR	fig|208964.1.peg.1175.
Organism-specific databases
PseudoCAP	PA1174.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG715033.
OMA	QFWHQLV.
Enzyme and pathway databases
BioCyc	PAER208964:PA1174-MONOMER.
BRENDA	1.7.99.4. 354.
Family and domain databases
HAMAP	MF_01630. Nitrate_reduct. [Tree]
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4_dom. IPR006655. Mopterin_OxRdtase_prok_CS. IPR010051. NO3_reductase_lsu_periplasm. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01706. NAPA. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. 1 hit. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAPA_PSEAE

Accession

Primary (citable) accession number: Q9I4G3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	March 1, 2001
Last modified:	February 9, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)