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Q9I4G3 (NAPA_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Periplasmic nitrate reductase

EC=1.7.99.4
Gene names
Name:napA
Ordered Locus Names:PA1174
OrganismPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]
Taxonomic identifier208964 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length829 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism By similarity. HAMAP-Rule MF_01630

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor. HAMAP-Rule MF_01630

Cofactor

Binds 1 4Fe-4S cluster By similarity. HAMAP-Rule MF_01630

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subunit structure

Interacts with NapB By similarity. HAMAP-Rule MF_01630

Subcellular location

Periplasm By similarity HAMAP-Rule MF_01630.

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 829805Periplasmic nitrate reductase HAMAP-Rule MF_01630
PRO_0000045995

Regions

Domain36 – 92574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding431Iron-sulfur (4Fe-4S) By similarity
Metal binding461Iron-sulfur (4Fe-4S) By similarity
Metal binding501Iron-sulfur (4Fe-4S) By similarity
Metal binding781Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9I4G3 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 4B543C34FEDB0398

FASTA82992,975
        10         20         30         40         50         60 
MNSPRPTPPP FAAAAAGLPI LVRASNLVTE ADVTSLVWNK APCRFCGTGC SVMVATRDGQ 

        70         80         90        100        110        120 
VVATHGDIKA EVNRGINCVK GYFLSKIMYG SDRLTRPLLR MKDGKFDKQG EFQPISWEQA 

       130        140        150        160        170        180 
FDIMAEKFKA ALKAKGPESV GMFGSGQWTV WEGYAANKLF KAGLRSNNID PNARHCMASA 

       190        200        210        220        230        240 
VMGFMRSFGM DEPMGCYDDI EATDSFVLWG SNMAEMHPVL WSRVTDRRLS APQVKVAVLS 

       250        260        270        280        290        300 
TFEHRSFELA DLPMVFKPQT DLIILNYIAN HIIESGAVNR DFVERHVRFA HGAEDIGYGL 

       310        320        330        340        350        360 
RPDDPLEKKA KNADKANTWS DIDFKAFAEF VKPYTLERTA RESGVPAERL KALAELYADP 

       370        380        390        400        410        420 
KRKVVSFWTM GFNQHTRGVW ANNLIYNIHL LTGKISEPGN SPFSLTGQPS ACGTAREVGT 

       430        440        450        460        470        480 
FSHRLPADLV VTNPKHRETA EKIWKVPAGT IQEKVGFHAV QQSRMLKDGV LNVYWTQVSN 

       490        500        510        520        530        540 
NMQAGPNVMQ EVLPGWRNPD NFVIVSDVYP TVSAQAADLI LPSAMWVEKE GAFGNAERRT 

       550        560        570        580        590        600 
QFWHQLVKAP GEAKSDLWQL VEFSKRFTTD EVWPAELLAK APELKGKTLY DVLFRNGQVD 

       610        620        630        640        650        660 
RFPASDLAKG YANDEVDAFG FYIQKGLFEE YAAFGRGHGH DLAPFDAYHE ARGLRWPVVD 

       670        680        690        700        710        720 
GKETRWRYRE GYDPYVSKGS GVQFYGYPDK KAIVFALPYE PPAEAPDQDY PFWLATGRVL 

       730        740        750        760        770        780 
EHWHTGSMTA RVPELYKAVP DALVYMHPED ARQLKLRRGS EVKVVSRRGE IRARVETRGR 

       790        800        810        820 
NKPPQGLVFV PFFDANKLIN KVTLDATDPI SKQTDYKKCA VRIELLNLA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE004091 Genomic DNA. Translation: AAG04563.1.
PIRF83499.
RefSeqNP_249865.1. NC_002516.2.

3D structure databases

ProteinModelPortalQ9I4G3.
SMRQ9I4G3. Positions 28-823.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING208964.PA1174.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG04563; AAG04563; PA1174.
GeneID878409.
KEGGpae:PA1174.
PATRIC19836666. VBIPseAer58763_1219.

Organism-specific databases

PseudoCAPPA1174.

Phylogenomic databases

eggNOGCOG0243.
HOGENOMHOG000031441.
KOK02567.
OMALVYMHPE.
OrthoDBEOG6CVV7G.
PhylomeDBQ9I4G3.

Family and domain databases

HAMAPMF_01630. Nitrate_reduct.
InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
[Graphical view]
PfamPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01706. NAPA. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAPA_PSEAE
AccessionPrimary (citable) accession number: Q9I4G3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: March 1, 2001
Last modified: July 9, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families