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Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein NapC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism.UniRule annotation

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi43 – 431Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi46 – 461Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi50 – 501Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi78 – 781Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.7.99.4UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:PA1174
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA1174.

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424UniRule annotationAdd
BLAST
Chaini25 – 829805Periplasmic nitrate reductasePRO_0000045995Add
BLAST

Interactioni

Subunit structurei

Interacts with NapB.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA1174.

Structurei

3D structure databases

ProteinModelPortaliQ9I4G3.
SMRiQ9I4G3. Positions 28-823.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 92574Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
InParanoidiQ9I4G3.
KOiK02567.
OMAiFAKRFTT.
OrthoDBiEOG6CVV7G.
PhylomeDBiQ9I4G3.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9I4G3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSPRPTPPP FAAAAAGLPI LVRASNLVTE ADVTSLVWNK APCRFCGTGC
60 70 80 90 100
SVMVATRDGQ VVATHGDIKA EVNRGINCVK GYFLSKIMYG SDRLTRPLLR
110 120 130 140 150
MKDGKFDKQG EFQPISWEQA FDIMAEKFKA ALKAKGPESV GMFGSGQWTV
160 170 180 190 200
WEGYAANKLF KAGLRSNNID PNARHCMASA VMGFMRSFGM DEPMGCYDDI
210 220 230 240 250
EATDSFVLWG SNMAEMHPVL WSRVTDRRLS APQVKVAVLS TFEHRSFELA
260 270 280 290 300
DLPMVFKPQT DLIILNYIAN HIIESGAVNR DFVERHVRFA HGAEDIGYGL
310 320 330 340 350
RPDDPLEKKA KNADKANTWS DIDFKAFAEF VKPYTLERTA RESGVPAERL
360 370 380 390 400
KALAELYADP KRKVVSFWTM GFNQHTRGVW ANNLIYNIHL LTGKISEPGN
410 420 430 440 450
SPFSLTGQPS ACGTAREVGT FSHRLPADLV VTNPKHRETA EKIWKVPAGT
460 470 480 490 500
IQEKVGFHAV QQSRMLKDGV LNVYWTQVSN NMQAGPNVMQ EVLPGWRNPD
510 520 530 540 550
NFVIVSDVYP TVSAQAADLI LPSAMWVEKE GAFGNAERRT QFWHQLVKAP
560 570 580 590 600
GEAKSDLWQL VEFSKRFTTD EVWPAELLAK APELKGKTLY DVLFRNGQVD
610 620 630 640 650
RFPASDLAKG YANDEVDAFG FYIQKGLFEE YAAFGRGHGH DLAPFDAYHE
660 670 680 690 700
ARGLRWPVVD GKETRWRYRE GYDPYVSKGS GVQFYGYPDK KAIVFALPYE
710 720 730 740 750
PPAEAPDQDY PFWLATGRVL EHWHTGSMTA RVPELYKAVP DALVYMHPED
760 770 780 790 800
ARQLKLRRGS EVKVVSRRGE IRARVETRGR NKPPQGLVFV PFFDANKLIN
810 820
KVTLDATDPI SKQTDYKKCA VRIELLNLA
Length:829
Mass (Da):92,975
Last modified:March 1, 2001 - v1
Checksum:i4B543C34FEDB0398
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG04563.1.
PIRiF83499.
RefSeqiNP_249865.1. NC_002516.2.
WP_010895543.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG04563; AAG04563; PA1174.
GeneIDi878409.
KEGGipae:PA1174.
PATRICi19836666. VBIPseAer58763_1219.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG04563.1.
PIRiF83499.
RefSeqiNP_249865.1. NC_002516.2.
WP_010895543.1. NC_002516.2.

3D structure databases

ProteinModelPortaliQ9I4G3.
SMRiQ9I4G3. Positions 28-823.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA1174.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG04563; AAG04563; PA1174.
GeneIDi878409.
KEGGipae:PA1174.
PATRICi19836666. VBIPseAer58763_1219.

Organism-specific databases

PseudoCAPiPA1174.

Phylogenomic databases

eggNOGiCOG0243.
HOGENOMiHOG000031441.
InParanoidiQ9I4G3.
KOiK02567.
OMAiFAKRFTT.
OrthoDBiEOG6CVV7G.
PhylomeDBiQ9I4G3.

Family and domain databases

HAMAPiMF_01630. Nitrate_reduct.
InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR010051. Periplasm_NO3_reductase_lsu.
[Graphical view]
PfamiPF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01706. NAPA. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiNAPA_PSEAE
AccessioniPrimary (citable) accession number: Q9I4G3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: March 1, 2001
Last modified: May 27, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.