Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Periplasmic nitrate reductase

Gene

napA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from NapB and catalyzes the reduction of nitrate to nitrite.UniRule annotation

Catalytic activityi

2 ferrocytochrome + nitrate + 2 H+ = 2 ferricytochrome + nitrite.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • Mo-bis(molybdopterin guanine dinucleotide)UniRule annotationNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi43Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi46Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi50Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi78Iron-sulfur (4Fe-4S)UniRule annotation1
Binding sitei80Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei147Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei172Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei176Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei370Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei374Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei480Mo-bis(molybdopterin guanine dinucleotide); via amide nitrogenUniRule annotation1
Binding sitei529Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei556Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei792Substrate; via amide nitrogenUniRule annotation1
Binding sitei800Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1
Binding sitei817Mo-bis(molybdopterin guanine dinucleotide)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciPAER208964:G1FZ6-1199-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductaseUniRule annotation (EC:1.9.6.1UniRule annotation)
Gene namesi
Name:napAUniRule annotation
Ordered Locus Names:PA1174
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA1174

Subcellular locationi

  • Periplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30UniRule annotationAdd BLAST30
ChainiPRO_000004599531 – 829Periplasmic nitrate reductaseUniRule annotationAdd BLAST799

Proteomic databases

PaxDbiQ9I4G3

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA1174

Structurei

3D structure databases

ProteinModelPortaliQ9I4G3
SMRiQ9I4G3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini36 – 924Fe-4S Mo/W bis-MGD-typeUniRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni209 – 216Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation8
Regioni240 – 244Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation5
Regioni259 – 261Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation3
Regioni506 – 507Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation2
Regioni716 – 725Mo-bis(molybdopterin guanine dinucleotide) bindingUniRule annotation10

Sequence similaritiesi

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/NapA/NarB subfamily.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107QIW Bacteria
COG0243 LUCA
HOGENOMiHOG000031441
InParanoidiQ9I4G3
KOiK02567
OMAiTQHWRQQ
PhylomeDBiQ9I4G3

Family and domain databases

HAMAPiMF_01630 Nitrate_reduct_NapA, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR027467 MopterinOxRdtase_cofactor_BS
IPR010051 Periplasm_NO3_reductase_lsu
PANTHERiPTHR11615:SF123 PTHR11615:SF123, 1 hit
PfamiView protein in Pfam
PF04879 Molybdop_Fe4S4, 1 hit
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01706 NAPA, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9I4G3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSPRPTPPP FAAAAAGLPI LVRASNLVTE ADVTSLVWNK APCRFCGTGC
60 70 80 90 100
SVMVATRDGQ VVATHGDIKA EVNRGINCVK GYFLSKIMYG SDRLTRPLLR
110 120 130 140 150
MKDGKFDKQG EFQPISWEQA FDIMAEKFKA ALKAKGPESV GMFGSGQWTV
160 170 180 190 200
WEGYAANKLF KAGLRSNNID PNARHCMASA VMGFMRSFGM DEPMGCYDDI
210 220 230 240 250
EATDSFVLWG SNMAEMHPVL WSRVTDRRLS APQVKVAVLS TFEHRSFELA
260 270 280 290 300
DLPMVFKPQT DLIILNYIAN HIIESGAVNR DFVERHVRFA HGAEDIGYGL
310 320 330 340 350
RPDDPLEKKA KNADKANTWS DIDFKAFAEF VKPYTLERTA RESGVPAERL
360 370 380 390 400
KALAELYADP KRKVVSFWTM GFNQHTRGVW ANNLIYNIHL LTGKISEPGN
410 420 430 440 450
SPFSLTGQPS ACGTAREVGT FSHRLPADLV VTNPKHRETA EKIWKVPAGT
460 470 480 490 500
IQEKVGFHAV QQSRMLKDGV LNVYWTQVSN NMQAGPNVMQ EVLPGWRNPD
510 520 530 540 550
NFVIVSDVYP TVSAQAADLI LPSAMWVEKE GAFGNAERRT QFWHQLVKAP
560 570 580 590 600
GEAKSDLWQL VEFSKRFTTD EVWPAELLAK APELKGKTLY DVLFRNGQVD
610 620 630 640 650
RFPASDLAKG YANDEVDAFG FYIQKGLFEE YAAFGRGHGH DLAPFDAYHE
660 670 680 690 700
ARGLRWPVVD GKETRWRYRE GYDPYVSKGS GVQFYGYPDK KAIVFALPYE
710 720 730 740 750
PPAEAPDQDY PFWLATGRVL EHWHTGSMTA RVPELYKAVP DALVYMHPED
760 770 780 790 800
ARQLKLRRGS EVKVVSRRGE IRARVETRGR NKPPQGLVFV PFFDANKLIN
810 820
KVTLDATDPI SKQTDYKKCA VRIELLNLA
Length:829
Mass (Da):92,975
Last modified:March 1, 2001 - v1
Checksum:i4B543C34FEDB0398
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA Translation: AAG04563.1
PIRiF83499
RefSeqiNP_249865.1, NC_002516.2
WP_010895543.1, NC_002516.2

Genome annotation databases

EnsemblBacteriaiAAG04563; AAG04563; PA1174
GeneIDi878409
KEGGipae:PA1174
PATRICifig|208964.12.peg.1219

Similar proteinsi

Entry informationi

Entry nameiNAPA_PSEAE
AccessioniPrimary (citable) accession number: Q9I4G3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: March 1, 2001
Last modified: March 28, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health