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Protein

N(G),N(G)-dimethylarginine dimethylaminohydrolase

Gene

PA1195

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA).2 Publications

Catalytic activityi

N(omega),N(omega)-dimethyl-L-arginine + H2O = dimethylamine + L-citrulline.3 Publications

Enzyme regulationi

Inhibited by zinc ions. Competitively inhibited by lysine.1 Publication

Kineticsi

  1. KM=44 µM for N-methyl-L-arginine1 Publication
  2. KM=39 µM for N,N-dimethyl-L-arginine1 Publication
  3. KM=26 µM for S-methyl-L-thiocitrulline1 Publication

    pH dependencei

    Optimum pH is 7-8.5.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei18Substrate; via carbonyl oxygen1 Publication1
    Binding sitei60Substrate1 Publication1
    Binding sitei85Substrate1 Publication1
    Binding sitei132Substrate1 Publication1
    Active sitei162Proton donor3 Publications1
    Metal bindingi162Zinc1 Publication1
    Binding sitei243Substrate; via carbonyl oxygen1 Publication1
    Active sitei249Nucleophile3 Publications1
    Metal bindingi249Zinc1 Publication1

    GO - Molecular functioni

    Keywordsi

    Molecular functionHydrolase
    LigandMetal-binding, Zinc

    Enzyme and pathway databases

    BioCyciPAER208964:G1FZ6-1220-MONOMER
    BRENDAi3.5.3.18 5087

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N(G),N(G)-dimethylarginine dimethylaminohydrolaseCurated (EC:3.5.3.183 Publications)
    Short name:
    DDAH1 Publication
    Short name:
    Dimethylarginine dimethylaminohydrolase1 Publication
    Alternative name(s):
    Dimethylargininase1 Publication
    Gene namesi
    Ordered Locus Names:PA1195
    OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
    Taxonomic identifieri208964 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    Proteomesi
    • UP000002438 Componenti: Chromosome

    Organism-specific databases

    PseudoCAPiPA1195

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi114E → Q: Abolishes enzyme activity. 1 Publication1
    Mutagenesisi162H → G or F: Abolishes enzyme activity. 2 Publications1
    Mutagenesisi249C → S: Abolishes enzyme activity. 2 Publications1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00003986771 – 254N(G),N(G)-dimethylarginine dimethylaminohydrolaseAdd BLAST254

    Proteomic databases

    PaxDbiQ9I4E3

    Interactioni

    Subunit structurei

    Homodimer.2 Publications

    Protein-protein interaction databases

    STRINGi208964.PA1195

    Structurei

    Secondary structure

    1254
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi4 – 8Combined sources5
    Helixi12 – 16Combined sources5
    Beta strandi21 – 23Combined sources3
    Helixi28 – 42Combined sources15
    Turni43 – 46Combined sources4
    Beta strandi48 – 52Combined sources5
    Turni59 – 62Combined sources4
    Turni64 – 67Combined sources4
    Beta strandi68 – 70Combined sources3
    Beta strandi75 – 77Combined sources3
    Helixi83 – 87Combined sources5
    Helixi88 – 99Combined sources12
    Beta strandi104 – 106Combined sources3
    Helixi115 – 117Combined sources3
    Beta strandi118 – 121Combined sources4
    Beta strandi124 – 129Combined sources6
    Beta strandi131 – 133Combined sources3
    Helixi135 – 147Combined sources13
    Beta strandi151 – 156Combined sources6
    Beta strandi158 – 162Combined sources5
    Helixi163 – 165Combined sources3
    Beta strandi166 – 169Combined sources4
    Beta strandi174 – 177Combined sources4
    Helixi179 – 181Combined sources3
    Helixi185 – 187Combined sources3
    Beta strandi190 – 194Combined sources5
    Helixi197 – 203Combined sources7
    Beta strandi206 – 208Combined sources3
    Beta strandi211 – 217Combined sources7
    Helixi219 – 226Combined sources8
    Turni227 – 229Combined sources3
    Beta strandi231 – 235Combined sources5
    Helixi238 – 241Combined sources4
    Turni242 – 244Combined sources3
    Turni247 – 250Combined sources4
    Beta strandi252 – 254Combined sources3

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1H70X-ray1.80A1-254[»]
    3BPBX-ray2.81A/B1-254[»]
    3RHYX-ray2.18A/B1-254[»]
    ProteinModelPortaliQ9I4E3
    SMRiQ9I4E3
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9I4E3

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni65 – 66Substrate binding1 Publication2

    Sequence similaritiesi

    Belongs to the DDAH family.Curated

    Phylogenomic databases

    eggNOGiENOG4106V2I Bacteria
    COG1834 LUCA
    HOGENOMiHOG000161035
    InParanoidiQ9I4E3
    KOiK01482
    OMAiCNGIALI
    PhylomeDBiQ9I4E3

    Family and domain databases

    InterProiView protein in InterPro
    IPR033199 DDAH/AD
    PANTHERiPTHR12737 PTHR12737, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9I4E3-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MFKHIIARTP ARSLVDGLTS SHLGKPDYAK ALEQHNAYIR ALQTCDVDIT
    60 70 80 90 100
    LLPPDERFPD SVFVEDPVLC TSRCAIITRP GAESRRGETE IIEETVQRFY
    110 120 130 140 150
    PGKVERIEAP GTVEAGDIMM VGDHFYIGES ARTNAEGARQ MIAILEKHGL
    160 170 180 190 200
    SGSVVRLEKV LHLKTGLAYL EHNNLLAAGE FVSKPEFQDF NIIEIPEEES
    210 220 230 240 250
    YAANCIWVNE RVIMPAGYPR TREKIARLGY RVIEVDTSEY RKIDGGVSCM

    SLRF
    Length:254
    Mass (Da):28,470
    Last modified:March 1, 2001 - v1
    Checksum:i28E9FC2F61E3EBE2
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE004091 Genomic DNA Translation: AAG04584.1
    PIRiF83497
    RefSeqiNP_249886.1, NC_002516.2
    WP_003086612.1, NC_002516.2

    Genome annotation databases

    EnsemblBacteriaiAAG04584; AAG04584; PA1195
    GeneIDi883112
    KEGGipae:PA1195
    PATRICifig|208964.12.peg.1241

    Similar proteinsi

    Entry informationi

    Entry nameiDDAH_PSEAE
    AccessioniPrimary (citable) accession number: Q9I4E3
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: March 1, 2001
    Last modified: May 23, 2018
    This is version 89 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
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