Q9I472 (COBO_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cob(I)yrinic acid a,c-diamide adenosyltransferase
EC=2.5.1.17

Alternative name(s):
Cob(I)alamin adenosyltransferase
Corrinoid adenosyltransferase

Gene names

Name:	cobO
Ordered Locus Names:	PA1272

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	203 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for both de novo synthesis of the corrin ring for the assimilation of exogenous corrinoids. Participates in the adenosylation of a variety of incomplete and complete corrinoids By similarity.
Catalytic activity	ATP + cob(I)yrinic acid a,c-diamide = triphosphate + adenosylcob(III)yrinic acid a,c-diamide. ATP + cobinamide = triphosphate + adenosylcobinamide.
Cofactor	Manganese By similarity.
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 2/7.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the Cob(I)alamin adenosyltransferase family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Manganese Nucleotide-binding
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cob(I)yrinic acid a,c-diamide adenosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 203	203	Cob(I)yrinic acid a,c-diamide adenosyltransferase		PRO_0000287788
Regions
	Nucleotide binding	41 – 47	7	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I472 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 2176356693732743	FASTA	203	22,292
	10 20 30 40 50 60 MNESPEKDQR HRERMERKKA VVDEKIAQAR DERGVLLVHS GNGKGKSSSA FGMVARALGH 70 80 90 100 110 120 GMKVGVVQFI KGAASTGEEA FFRRFPEEVS YHVMGEGFTW ETQDRQRDIA KAEAAWKVAA 130 140 150 160 170 180 QLLADPDVGL VVLDELNIAL KHGYLELDRV LADIQARPAM QHVVVTGRGA QPGMIEAADT 190 200 VTEMSLVKHA FKAGIKAQKG VEF

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG04661.1.
PIR	F83485.
RefSeq	NP_249963.1. NC_002516.2.
3D structure databases
HSSP	HSSP built from PDB template 1G64 based on UniProtKB P31570.
ProteinModelPortal	Q9I472.
SMR	Q9I472. Positions 33-203.
ModBase	Search...
Protein-protein interaction databases
STRING	208964.PA1272.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	881408.
KEGG	pae:PA1272.
PATRIC	19836872. VBIPseAer58763_1322.
Organism-specific databases
PseudoCAP	PA1272.
Phylogenomic databases
eggNOG	COG2109.
HOGENOM	HOG000260311.
KO	K00798.
OMA	WHTMGEG.
ProtClustDB	PRK05986.
Enzyme and pathway databases
UniPathway	UPA00148; UER00233.
Family and domain databases
InterPro	IPR003724. AdoCbl_synth_CblAdoTrfase_CobA. IPR025826. Co_AT_N_dom. [Graphical view]
Pfam	PF12557. Co_AT_N. 1 hit. PF02572. CobA_CobO_BtuR. 1 hit. [Graphical view]
PIRSF	PIRSF015617. Adensltrnsf_CobA. 1 hit.
TIGRFAMs	TIGR00708. cobA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COBO_PSEAE

Accession

Primary (citable) accession number: Q9I472

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program