Q9I469 (COBD_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cobalamin biosynthesis protein CobD

Gene names

Name:	cobD
Ordered Locus Names:	PA1275

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group By similarity. HAMAP-Rule MF_00024
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis. HAMAP-Rule MF_00024
Subcellular location	Cell membrane; Multi-pass membrane protein Potential HAMAP-Rule MF_00024.
Sequence similarities	Belongs to the CobD/CbiB family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis Porphyrin biosynthesis
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway porphyrin-containing compound biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cobalamin-transporting ATPase activity Inferred from electronic annotation. Source: HAMAP threonine-phosphate decarboxylase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Cobalamin biosynthesis protein CobD HAMAP-Rule MF_00024		PRO_0000287753
Regions
	Transmembrane	59 – 79	21	Helical; Potential
	Transmembrane	81 – 101	21	Helical; Potential
	Transmembrane	153 – 173	21	Helical; Potential
	Transmembrane	241 – 261	21	Helical; Potential
	Transmembrane	292 – 312	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9I469 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: C74A880A7F474347	FASTA	312	33,873
	10 20 30 40 50 60 MSALLGILGV VLDARFGEPQ GWHPLVAFGR LADRLERRFN RSRLAGEADP GVGWRSHGVT 70 80 90 100 110 120 AWVLAVLPLT LLAWALAELP YLGWLVQALA LYAALGLRSL GEHAEPVALA LRAGDLDEAR 130 140 150 160 170 180 RRVGYLVSRD TRELDQAGVA RAATESVLEN GSDAVYAALF WFAVAGAPGV VLYRLSNTLD 190 200 210 220 230 240 AMWGYRNARF ERFGWAAAKI DDILNYLPAR LVALTYALLG DTRQALRCWR EQAPQWDSPN 250 260 270 280 290 300 AGPVMAAGAG ALGVFLGGAA IYHGELHQRP LLGSGPQPQG RDIYRALALV RQGVLLWLLV 310 LAGLWAMGWL HA

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG04664.1.
PIR	A83486.
RefSeq	NP_249966.1. NC_002516.2.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	208964.PA1275.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	881384.
KEGG	pae:PA1275.
PATRIC	19836878. VBIPseAer58763_1325.
Organism-specific databases
PseudoCAP	PA1275.
Phylogenomic databases
eggNOG	COG1270.
HOGENOM	HOG000290070.
KO	K02227.
OMA	RWHPLVG.
ProtClustDB	PRK01209.
Enzyme and pathway databases
UniPathway	UPA00148.
Family and domain databases
HAMAP	MF_00024. CobD_CbiB.
InterPro	IPR004485. Cobalamin_biosynth_CobD/CbiB. [Graphical view]
Pfam	PF03186. CobD_Cbib. 1 hit. [Graphical view]
TIGRFAMs	TIGR00380. cobD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COBD_PSEAE

Accession

Primary (citable) accession number: Q9I469

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	March 1, 2001
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program