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Protein

Bifunctional adenosylcobalamin biosynthesis protein CobP

Gene

cobP

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes ATP-dependent phosphorylation of adenosylcobinamide and addition of GMP to adenosylcobinamide phosphate.By similarity

Catalytic activityi

ATP or GTP + adenosylcobinamide = adenosylcobinamide phosphate + ADP or GDP.
GTP + adenosylcobinamide phosphate = diphosphate + adenosylcobinamide-GDP.

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 5 and 6 of the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Cob(I)yrinic acid a,c-diamide adenosyltransferase (cobO)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Bifunctional adenosylcobalamin biosynthesis protein CobP (cobP)
  6. Bifunctional adenosylcobalamin biosynthesis protein CobP (cobP)
  7. Adenosylcobinamide-GDP ribazoletransferase (cobS)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei48GMP-histidine intermediateBy similarity1
Binding sitei60GTPBy similarity1
Binding sitei82GTP; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi7 – 14GTPBy similarity8
Nucleotide bindingi32 – 34GTPBy similarity3
Nucleotide bindingi49 – 52GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00148; UER00236.
UPA00148; UER00237.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional adenosylcobalamin biosynthesis protein CobP
Alternative name(s):
Adenosylcobinamide kinase (EC:2.7.1.156)
Adenosylcobinamide-phosphate guanylyltransferase (EC:2.7.7.62)
Gene namesi
Name:cobP
Ordered Locus Names:PA1278
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA1278.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002877891 – 173Bifunctional adenosylcobalamin biosynthesis protein CobPAdd BLAST173

Proteomic databases

PaxDbiQ9I466.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi208964.PA1278.

Structurei

3D structure databases

ProteinModelPortaliQ9I466.
SMRiQ9I466.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the CobU/CobP family.Curated

Phylogenomic databases

eggNOGiENOG4108YZT. Bacteria.
COG2087. LUCA.
HOGENOMiHOG000286613.
InParanoidiQ9I466.
KOiK02231.
OMAiLWLTNHL.
PhylomeDBiQ9I466.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003203. Cobinamide_kinase/P_G-Trfase.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF02283. CobU. 1 hit.
[Graphical view]
PIRSFiPIRSF006135. CobU. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9I466-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRDLILGGAR SGKSRLAERL AAESGLAVSY IATAQAGDGE MGRRIAEHRA
60 70 80 90 100
RRPAHWRTLE EPLALAATLR SEAEAGRCLL VDCLTLWLTN LLLCDDPQRL
110 120 130 140 150
DGEREALLEC LGELPGRIIL VSNETGLGVV PLGELSRRYV DEAGWLHQAI
160 170
AERCERVTFT VAGLPMPLKG EPL
Length:173
Mass (Da):18,862
Last modified:March 1, 2001 - v1
Checksum:iEE3DDDC3858CC8DF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG04667.1.
PIRiD83486.
RefSeqiNP_249969.1. NC_002516.2.
WP_003082599.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG04667; AAG04667; PA1278.
GeneIDi881443.
KEGGipae:PA1278.
PATRICi19836884. VBIPseAer58763_1328.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG04667.1.
PIRiD83486.
RefSeqiNP_249969.1. NC_002516.2.
WP_003082599.1. NC_002516.2.

3D structure databases

ProteinModelPortaliQ9I466.
SMRiQ9I466.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA1278.

Proteomic databases

PaxDbiQ9I466.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG04667; AAG04667; PA1278.
GeneIDi881443.
KEGGipae:PA1278.
PATRICi19836884. VBIPseAer58763_1328.

Organism-specific databases

PseudoCAPiPA1278.

Phylogenomic databases

eggNOGiENOG4108YZT. Bacteria.
COG2087. LUCA.
HOGENOMiHOG000286613.
InParanoidiQ9I466.
KOiK02231.
OMAiLWLTNHL.
PhylomeDBiQ9I466.

Enzyme and pathway databases

UniPathwayiUPA00148; UER00236.
UPA00148; UER00237.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003203. Cobinamide_kinase/P_G-Trfase.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF02283. CobU. 1 hit.
[Graphical view]
PIRSFiPIRSF006135. CobU. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCOBP_PSEAE
AccessioniPrimary (citable) accession number: Q9I466
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: March 1, 2001
Last modified: November 2, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.