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Protein

Adenosylcobinamide-GDP ribazoletransferase

Gene

cobS

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Joins adenosylcobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin 5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-phosphate.UniRule annotation

Catalytic activityi

Adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin.UniRule annotation
Adenosylcobinamide-GDP + alpha-ribazole 5'-phosphate = GMP + adenosylcobalamin 5'-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: adenosylcobalamin biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Cob(I)yrinic acid a,c-diamide adenosyltransferase (cobO)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Bifunctional adenosylcobalamin biosynthesis protein CobP (cobP)
  6. Bifunctional adenosylcobalamin biosynthesis protein CobP (cobP)
  7. Adenosylcobinamide-GDP ribazoletransferase (cobS)
This subpathway is part of the pathway adenosylcobalamin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes adenosylcobalamin from cob(II)yrinate a,c-diamide, the pathway adenosylcobalamin biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Cobalamin biosynthesis

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

UniPathwayiUPA00148; UER00238.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosylcobinamide-GDP ribazoletransferaseUniRule annotation (EC:2.7.8.26UniRule annotation)
Alternative name(s):
Cobalamin synthaseUniRule annotation
Cobalamin-5'-phosphate synthaseUniRule annotation
Gene namesi
Name:cobSUniRule annotation
Ordered Locus Names:PA1281
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA1281.

Subcellular locationi

  • Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei31 – 5121HelicalUniRule annotationAdd
BLAST
Transmembranei61 – 8121HelicalUniRule annotationAdd
BLAST
Transmembranei113 – 13321HelicalUniRule annotationAdd
BLAST
Transmembranei138 – 15821HelicalUniRule annotationAdd
BLAST
Transmembranei192 – 21221HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 245245Adenosylcobinamide-GDP ribazoletransferasePRO_0000146887Add
BLAST

Proteomic databases

PaxDbiQ9I463.

Interactioni

Protein-protein interaction databases

STRINGi208964.PA1281.

Family & Domainsi

Sequence similaritiesi

Belongs to the CobS family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105GN9. Bacteria.
COG0368. LUCA.
HOGENOMiHOG000228087.
InParanoidiQ9I463.
KOiK02233.
OMAiEPHYLNA.
OrthoDBiEOG6QCDBK.

Family and domain databases

HAMAPiMF_00719. CobS.
InterProiIPR003805. CobS.
[Graphical view]
PfamiPF02654. CobS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00317. cobS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9I463-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREALRSLLV ALQFLTRLPV RLSAMPTPEQ FGRAVLCYPL VGVLIGVVLY
60 70 80 90 100
AAARSLDGTP PLLQAALLLS LWVALSGALH LDGLADMADA WVGGLGDRER
110 120 130 140 150
TLAIMKDPRS GPVAVVVLVL VLLLKFSALA ALLGQGEAGL LPLAPWLARS
160 170 180 190 200
SLPLLFLTTP YARPGGLGQA IAEHLPARSL PWVLGVSFGL ALAFGLAGLL
210 220 230 240
ALLVTLMLFA WLRSRFLARL GGTTGDTAGA LVELTECAVL VALAL
Length:245
Mass (Da):25,639
Last modified:March 1, 2001 - v1
Checksum:iF113DAD2CBF91BCC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG04670.1.
PIRiG83486.
RefSeqiNP_249972.1. NC_002516.2.
WP_003086787.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG04670; AAG04670; PA1281.
GeneIDi881439.
KEGGipae:PA1281.
PATRICi19836890. VBIPseAer58763_1331.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG04670.1.
PIRiG83486.
RefSeqiNP_249972.1. NC_002516.2.
WP_003086787.1. NC_002516.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA1281.

Proteomic databases

PaxDbiQ9I463.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG04670; AAG04670; PA1281.
GeneIDi881439.
KEGGipae:PA1281.
PATRICi19836890. VBIPseAer58763_1331.

Organism-specific databases

PseudoCAPiPA1281.

Phylogenomic databases

eggNOGiENOG4105GN9. Bacteria.
COG0368. LUCA.
HOGENOMiHOG000228087.
InParanoidiQ9I463.
KOiK02233.
OMAiEPHYLNA.
OrthoDBiEOG6QCDBK.

Enzyme and pathway databases

UniPathwayiUPA00148; UER00238.

Family and domain databases

HAMAPiMF_00719. CobS.
InterProiIPR003805. CobS.
[Graphical view]
PfamiPF02654. CobS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00317. cobS. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiCOBS_PSEAE
AccessioniPrimary (citable) accession number: Q9I463
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: March 1, 2001
Last modified: November 11, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.