Reviewed, UniProtKB/Swiss-Prot Q9I406 (GGT_PSEAE)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyltranspeptidase
    EC=2.3.2.2
Cleaved into the following 2 chains:
    1- Recommended name:
            Gamma-glutamyltranspeptidase large chain
    2- Recommended name:
            Gamma-glutamyltranspeptidase small chain

Gene names

Name:	ggt
Ordered Locus Names:	PA1338

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	557 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(5-L-glutamyl)-peptide + an amino acid = peptide + 5-L-glutamyl amino acid.
Pathway	Sulfur metabolism; glutathione metabolism.
Subunit structure	This enzyme consists of two polypeptide chains, which are synthesized in precursor form from a single polypeptide By similarity.
Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the gamma-glutamyltransferase family.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Cellular component	Periplasm
Domain	Signal
Molecular function	Acyltransferase Transferase
PTM	Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW gamma-glutamyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – ?		Gamma-glutamyltranspeptidase large chain		PRO_0000287773
	Chain	? – 557		Gamma-glutamyltranspeptidase small chain		PRO_0000287774

Sequences

	Sequence		Length	Mass (Da)
	Q9I406-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: FDE0DFE595665510	FASTA	557	59,874
	10 20 30 40 50 60 MQPVLFRTLS LGVAIAAASS SAFAATLDGG AVAAPDEYGA KVAAQILKAG GNAVDAAVAT 70 80 90 100 110 120 AFTLAVTYPE AGNIGGGGFM TLYMDGKPYF LDYREVAPKA ASKTMYLDDK GEVIENLSLV 130 140 150 160 170 180 GAKAAGVPGT VMGLWEAHKR FGKLPWSELL TPAIGYAQKG FKVADKQFQY RQDAVALFNG 190 200 210 220 230 240 KTNFGDYFGH MKAGEAFLQP DLAKTLERIA DKGPDEFYKG HTADLLVAQM QQDKGLITHQ 250 260 270 280 290 300 DLADYKVRWR EPMRVDWQGN TLYTAPLPSS GGIALAQLLG IKENRAADFK GVELNSARYI 310 320 330 340 350 360 HLLAEIEKRV FADRADYLGD PDFSKVPVAR LTDPAYLKQR AAEVNPTAIS PTEKVRPGLE 370 380 390 400 410 420 PHQTTHFSIV DADGNAVSNT YTLNWDFGSG VVVKGAGFLL NDEMDDFSAK PGVANAFGVV 430 440 450 460 470 480 GSDANAIEPG KRMLSSMSPS IVTRDGKVSL VVGTPGGSRI FTSIFQVLNN IYDFHLPLEK 490 500 510 520 530 540 AVAAQRVHHQ LLPKDTIYYD AYAPLAGKVA EELKAMGYTL EDQGWNMGDI QAIRVDGKAL 550 ETASDPRGRG VGLVVKP

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG04727.1.
PIR	D83478.
RefSeq	NP_250029.1.
3D structure databases
HSSP	HSSP built from PDB template 2DG5 based on UniProtKB P18956.
SMR	Q9I406. Positions 28-359.
ModBase	Search...
Protein family/group databases
MEROPS	T03.001.
Genome annotation databases
GeneID	882262.
GenomeReviews	Gene locus PA1338 in contig AE004091_GR.
KEGG	pae:PA1338.
Organism-specific databases
PseudoCAP	PA1338.
CMR	Search...
Phylogenomic databases
HOGENOM	HBG738311.
OMA	PTILTKD.
Enzyme and pathway databases
BioCyc	PAER208964:PA1338-MONOMER.
BRENDA	2.3.2.2. 354.
Family and domain databases
InterPro	IPR000101. GGT_peptidase. [Graphical view]
PANTHER	PTHR11686. GGT_peptidase. 1 hit.
Pfam	PF01019. G_glu_transpept. 1 hit. [Graphical view]
PRINTS	PR01210. GGTRANSPTASE.
TIGRFAMs	TIGR00066. g_glut_trans. 1 hit.
PROSITE	PS00462. G_GLU_TRANSPEPTIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GGT_PSEAE

Accession

Primary (citable) accession number: Q9I406

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	March 1, 2001
Last modified:	February 9, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)