Reviewed, UniProtKB/Swiss-Prot Q9I3N7 (CCMA_PSEAE)

Names and origin

Protein names

Recommended name:
    Cytochrome c biogenesis ATP-binding export protein ccmA
    EC=3.6.3.41
Alternative name(s):
    Heme exporter protein A

Gene names

Name:	ccmA
Ordered Locus Names:	PA1475

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	233 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Part of the ABC transporter complex ccmAB involved in the biogenesis of c-type cytochromes; once thought to export heme, this seems not to be the case, but its exact role is uncertain. Responsible for energy coupling to the transport system By similarity.
Catalytic activity	ATP + H2O + heme(In) = ADP + phosphate + heme(Out). HAMAP MF_01707
Subunit structure	The complex is composed of two ATP-binding proteins (ccmA) and two transmembrane proteins (ccmB) Probable.
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the ABC transporter superfamily. CcmA exporter (TC 3.A.1.107) family. [View classification] Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cytochrome complex assembly Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: HAMAP
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW heme-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 233	233	Cytochrome c biogenesis ATP-binding export protein ccmA HAMAP MF_01707		PRO_0000092195
Regions
	Domain	14 – 233	220	ABC transporter
	Nucleotide binding	46 – 53	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9I3N7-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 5FC03789462DF880	FASTA	233	24,996
	10 20 30 40 50 60 MSDPAEVPTA SPLLETVGLA CERDWRMLFE RLDLRLAAGE MLQVVGPNGS GKTSLLRLLS 70 80 90 100 110 120 GLMQPTAGEV RLNGRPLAEQ RGELARSLLW IGHAAGIKGL LSAEENLTWL CALHQPASRE 130 140 150 160 170 180 AIWQALADVG LRGFEDVPCH TLSAGQQRRV ALARLYLDAP PLWILDEPFT ALDKQGVAQL 190 200 210 220 230 ETHLAGHCQR GGMVVLTTHH SLQQVPAGYR ELDLGALKAA SGATVEPALG DPA

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004091 Genomic DNA. Translation: AAG04864.1.
PIR	G83462.
RefSeq	NP_250166.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	880978.
GenomeReviews	Gene locus PA1475 in contig AE004091_GR.
KEGG	pae:PA1475.
NMPDR	fig|208964.1.peg.1476.
Organism-specific databases
PseudoCAP	PA1475.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9I3N7.
OMA	QTGVKRE.
Enzyme and pathway databases
BioCyc	PAER208964:PA1475-MON.
BRENDA	3.6.3.41. 354.
Family and domain databases
HAMAP	MF_01707. [Tree]
InterPro	IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR005895. ABC_transptr_haem_export_CcmA. IPR003593. ATPase_AAA+_core. [Graphical view]
PANTHER	PTHR19222:SF5. CcmA. 1 hit.
Pfam	PF00005. ABC_tran. 1 hit. [Graphical view]
ProDom	PD000006. ABC_transporter. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01189. ccmA. 1 hit.
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. PS51243. CCMA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CCMA_PSEAE

Accession

Primary (citable) accession number: Q9I3N7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)