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Protein

Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase

Gene

PA1772

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2-oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions (By similarity).By similarity

Catalytic activityi

4-hydroxy-4-methyl-2-oxoglutarate = 2 pyruvate.
Oxaloacetate = pyruvate + CO2.

Cofactori

a divalent metal cationBy similarityNote: Divalent metal cation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei97 – 971SubstrateBy similarity
Metal bindingi98 – 981Divalent metal cationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase (EC:4.1.3.17)
Short name:
HMG aldolase
Alternative name(s):
Oxaloacetate decarboxylase (EC:4.1.1.3)
Short name:
OAA decarboxylase
Regulator of ribonuclease activity homolog
RraA-like protein
Gene namesi
Ordered Locus Names:PA1772
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA1772.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 162162Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolasePRO_0000209629Add
BLAST

Proteomic databases

PaxDbiQ9I2W7.

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi208964.PA1772.

Structurei

Secondary structure

1
162
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 117Combined sources
Turni13 – 153Combined sources
Beta strandi32 – 409Combined sources
Helixi46 – 527Combined sources
Beta strandi59 – 646Combined sources
Beta strandi69 – 735Combined sources
Helixi76 – 849Combined sources
Beta strandi89 – 968Combined sources
Helixi99 – 1024Combined sources
Beta strandi105 – 11410Combined sources
Beta strandi126 – 1283Combined sources
Beta strandi131 – 1333Combined sources
Beta strandi136 – 1383Combined sources
Beta strandi142 – 1465Combined sources
Beta strandi151 – 1566Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3C8OX-ray1.90A/B1-162[»]
ProteinModelPortaliQ9I2W7.
SMRiQ9I2W7. Positions 1-161.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9I2W7.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni75 – 784Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class II aldolase/RraA-like family.Curated

Phylogenomic databases

eggNOGiENOG4108YYX. Bacteria.
COG0684. LUCA.
HOGENOMiHOG000252803.
InParanoidiQ9I2W7.
KOiK02553.
OMAiLQICRQS.
OrthoDBiEOG6Z3KKK.
PhylomeDBiQ9I2W7.

Family and domain databases

Gene3Di3.50.30.40. 1 hit.
InterProiIPR010203. RraA.
IPR005493. RraA/RraA-like.
[Graphical view]
PfamiPF03737. Methyltransf_6. 1 hit.
[Graphical view]
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9I2W7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHYVTPDLCD AYPELVQVVE PMFSNFGGRD SFGGEIVTIK CFEDNSLVKE
60 70 80 90 100
QVDKDGKGKV LVVDGGGSLR RALLGDMLAE KAAKNGWEGI VVYGCIRDVD
110 120 130 140 150
VIAQTDLGVQ ALASHPLKTD KRGIGDLNVA VTFGGVTFRP GEFVYADNNG
160
IIVSPQALKM PE
Length:162
Mass (Da):17,404
Last modified:March 1, 2001 - v1
Checksum:i4F682F7A485DE1EB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG05161.1.
PIRiB83423.
RefSeqiNP_250463.1. NC_002516.2.
WP_003087823.1. NZ_ASJY01000250.1.

Genome annotation databases

EnsemblBacteriaiAAG05161; AAG05161; PA1772.
GeneIDi880779.
KEGGipae:PA1772.
PATRICi19837903. VBIPseAer58763_1837.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004091 Genomic DNA. Translation: AAG05161.1.
PIRiB83423.
RefSeqiNP_250463.1. NC_002516.2.
WP_003087823.1. NZ_ASJY01000250.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3C8OX-ray1.90A/B1-162[»]
ProteinModelPortaliQ9I2W7.
SMRiQ9I2W7. Positions 1-161.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA1772.

Proteomic databases

PaxDbiQ9I2W7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG05161; AAG05161; PA1772.
GeneIDi880779.
KEGGipae:PA1772.
PATRICi19837903. VBIPseAer58763_1837.

Organism-specific databases

PseudoCAPiPA1772.

Phylogenomic databases

eggNOGiENOG4108YYX. Bacteria.
COG0684. LUCA.
HOGENOMiHOG000252803.
InParanoidiQ9I2W7.
KOiK02553.
OMAiLQICRQS.
OrthoDBiEOG6Z3KKK.
PhylomeDBiQ9I2W7.

Miscellaneous databases

EvolutionaryTraceiQ9I2W7.

Family and domain databases

Gene3Di3.50.30.40. 1 hit.
InterProiIPR010203. RraA.
IPR005493. RraA/RraA-like.
[Graphical view]
PfamiPF03737. Methyltransf_6. 1 hit.
[Graphical view]
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiRRAAH_PSEAE
AccessioniPrimary (citable) accession number: Q9I2W7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: March 1, 2001
Last modified: April 13, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.