Reviewed, UniProtKB/Swiss-Prot Q9I2V5 (ACON2_PSEAE)

Names and origin

Protein names

Recommended name:
    Aconitate hydratase 2
      Short name=Aconitase 2
    EC=4.2.1.3
Alternative name(s):
    Citrate hydro-lyase 2

Gene names

Name:	acnB
Ordered Locus Names:	PA1787

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	869 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW aconitate hydratase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 869	869	Aconitate hydratase 2		PRO_0000287740
Sites
	Metal binding	713	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	772	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	775	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9I2V5-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: D8026242DFAED72B	FASTA	869	93,628
	10 20 30 40 50 60 MLEAYRKHVE ERAAQGVVPQ PLNAEQTAGL VELLKNPPAG EEEFLLDLIT NRVPPGVDEA 70 80 90 100 110 120 AYVKAGFLSA IVKGEATSPL IDKQRAAELL GTMQGGYNIA TLVELLDDAE LANTAAEQLK 130 140 150 160 170 180 HTLLMFDAFH DVAERAKKGN AAAKSVLQSW ADGEWFKAKP EVPEKLTLTV FKVPGETNTD 190 200 210 220 230 240 DLSPAPDAWS RPDIPLHALA MLKMARDGIE PVQPGSVGPL KQIEAVKAKG FPVAYVGDVV 250 260 270 280 290 300 GTGSSRKSAT NSVLWFFGDD IPFVPNKRAG GFCFGTKIAP IFYNTMEDAG ALPIEFDCTN 310 320 330 340 350 360 LAMGDVIDVY PYEGKVVRHD SGEVVTTFEL KTPVLLDEVR AGGRIPLIVG RGLTEKARAE 370 380 390 400 410 420 LGLGASDLFR KPEAPADSGK GFTLAQKMVG RACGLPEGQG VRPGTYCEPK MTTVGSQDTT 430 440 450 460 470 480 GPMTRDELKD LACLGFSADL VMQSFCHTAA YPKPIDVKTH HTLPDFIMTR GGVSLRPGDG 490 500 510 520 530 540 IIHSWLNRML LPDTVGTGGD SHTRFPIGIS FPAGSGLVAF AAATGVMPLD MPESVLVRFK 550 560 570 580 590 600 GKLQPGITLR DLVHAIPYYA IQQGLLTVEK KGKKNIFSGR ILEIEGLNDL TVEQAFELSD 610 620 630 640 650 660 ASAERSAAGC TIKLPEQAIA EYLKSNITLL RWMIGEGYGD PRTLERRAQA MEAWLAKPEL 670 680 690 700 710 720 LEADKDAEYA AVIEIDLADV KEPVLCAPND PDDARLLSSV QGRKIDEVFI GSCMTNIGHF 730 740 750 760 770 780 RAAGKLLDKV KGGIPTRLWL APPTKMDAHQ LTEEGYYGIY GKAGARMEMP GCSLCMGNQA 790 800 810 820 830 840 RVQTGSTVVS TSTRNFPNRL GDATDVFLAS AELAAVSSIL GKLPTVEEYM AYAKDIDSMA 850 860 ADVYRYLSFD QIAEFREAAA NAKIPVVQA

References

[1]

"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE004091 Genomic DNA. Translation: AAG05176.1.
PIR	G83422.
RefSeq	NP_250478.1.
3D structure databases
HSSP	HSSP built from PDB template 1L5J based on UniProtKB P36683.
SMR	Q9I2V5. Positions 1-859.
ModBase	Search...
Genome annotation databases
GeneID	879984.
GenomeReviews	Gene locus PA1787 in contig AE004091_GR.
KEGG	pae:PA1787.
Organism-specific databases
PseudoCAP	PA1787.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9I2V5.
OMA	VELLGTM.
Enzyme and pathway databases
BioCyc	PAER208964:PA1787-MON.
BRENDA	4.2.1.3. 354.
Family and domain databases
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015937. Aconitase-like_core. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR004406. Aconitase_B_bac. IPR015930. Aconitase_B_FeS-bd_bac. IPR015933. Aconitase_B_HEAT-like_bac. IPR015929. Aconitase_B_N_bac. [Graphical view]
Gene3D	G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 2 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit. G3DSA:1.25.40.160. Aconitase_B_HEAT-like_bac. 1 hit.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF3. Aconitase_2. 1 hit.
Pfam	PF00330. Aconitase. 1 hit. PF06434. Aconitase_2_N. 1 hit. [Graphical view]
PIRSF	PIRSF036687. AcnB. 1 hit.
ProDom	PD000511. Aconitase_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00117. acnB. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACON2_PSEAE

Accession

Primary (citable) accession number: Q9I2V5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)